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Water solubility

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Reference
Endpoint:
water solubility
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from 2017-07-21 to 2017-07-26
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 105 (Water Solubility)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method A.6 (Water Solubility)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EPA OPPTS 830.7840 (Water Solubility)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Type of method:
flask method
Key result
Water solubility:
3.12 g/L
Conc. based on:
test mat.
Incubation duration:
72 h
Temp.:
20 °C
pH:
>= 4.87 - <= 5.53

Preliminary Test

The water solubility of the test item estimated to be was 3.6 g/L in a preliminary test and therefore the shake-flask method was used in the main test.

Main test

The calibration solutions were prepared in 0.2 – 100 μg/mL range. The calibration samples were analysed by HPLC-UV method with three replicate injections from each. Measured concentrations of the test item are summarised in Table 1. The repeatability of the determination was < 15% and thus within the range required by the guidelines.

Table 1: Results of the determination of water solubility of the test item

Sampling time Measured concentration of test item
(g/L)		 Measured pH
Test vessels Mean with the 95%
confidence intervals
After 24 h 3.05 3.10 ± 0.12 5.02
3.10 5.14
3.15 5.19
After 48 h 3.09 3.12 ± 0.09 5.05
3.16 4.95
3.10 4.90
After 72 h 3.08 3.14 ± 0.14 5.53
3.15 4.87
3.19 4.93
Solubility of the test item (g/L): 3.12 ± 0.05  - 
RSD%: 1  - 
pH of ultra-pure water:  -  6.71 - 6.76
Conclusions:
The water solubility of test item was determined to be 3.12 ± 0.05 g/L (95% confidence interval) at 20 °C ± 0.5 °C using the shake-flask method for the performance of the main test.
Executive summary:

A study was conducted according to OECD TG 105, Regulation (EC) No. 440/2008 method A.6 and OPPTS 830.7840 in order to determine the water solubility of the test item. Since in the preliminary test the water solubility was 3.6 g/L the shake flask method was used for the main test. The calibration solutions were prepared in 0.2 – 100 μg/mL range. The calibration samples were analysed by HPLC-UV method with three replicate injections from each. The water solubility of test item was determined to be 3.12 ± 0.05 g/L (95% confidence interval) at 20 °C ± 0.5 °C and 72 h sampling time (reference 4.8-1).

Description of key information

The water solubility of test item was determined to be 3.12 ± 0.05 g/L (95% confidence interval) at 20 °C ± 0.5 °C using the shake-flask method for the performance of the main test.

Key value for chemical safety assessment

Water solubility:
3.12 g/L
at the temperature of:
20 °C

Additional information

A study was conducted according to OECD TG 105, Regulation (EC) No. 440/2008 method A.6 and OPPTS 830.7840 in order to determine the water solubility of the test item. Since in the preliminary test the water solubility was 3.6 g/L the shake flask method was used for the main test. The calibration solutions were prepared in 0.2 – 100 μg/mL range. The calibration samples were analysed by HPLC-UV method with three replicate injections from each. The water solubility of test item was determined to be 3.12 ± 0.05 g/L (95% confidence interval) at 20 °C ± 0.5 °C and 72 h sampling time (reference 4.8-1).