Orthosulfamuron

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Qualifier:

according to guideline

Guideline:

other: OECD 202 (Draft proposal for updating guideline 202, October 2000) OECD 219 (Proposal for a new guideline, 2001) OECD 218 (Proposal for a new guideline, 2001)

GLP compliance:

yes

Specific details on test material used for the study:

Test material:
IR5878
Batch number: G 009/02
Purity: 98.56 ± 0.19 %

Details on sampling:

In a limit test 4 groups of 5 first instar larvae of Chironomus riparius were exposed to IR5878 at the nominal concentration of 100 mg/L for 48 hours. 4 additional groups of 5 animals where used as control.
Duplicate samples were taken at the start of the test from the freshly prepared test medium of the only test concentration and the control.
For the determination of the stability of the test item under the test conditions, respectively the maintenance of the test item concentration during the test period, duplicate samples from the test medium of the only concentration and the control were collected at the end of the test (after 48 hours) by pouring together the contents of the test beakers of each treatment.

Details on test solutions:

In a limit test 4 groups of 5 first instar larvae of Chironomus riparius were exposed to IR5878 at the nominal concentration of 100 mg/L for 48 hours

Test organisms (species):

other: Chironomus riparius

Details on test organisms:

Test animals:
Species and Strain: Male and Female Chironomus riparius
Age: first instar larvae (only fresh egg masses have been used as source for the test animals. The larvae were 3-4 days old at test start).
Housing: 5 animals/glass beaker of 100 mL.

Total exposure duration:

48 h

Test temperature:

Water temperature: 19 ÷ 20°C

pH:

pH water: 7.5 ÷ 8.1

Dissolved oxygen:

Dissolved oxygen: ≥ 8.1 mg/L

Nominal and measured concentrations:

The analytically determined mean IR5878 concentrations in the analysed test media varied in the range from 104% to 106% on the nominal values. In the test media the test item was sufficiently stable during the test period of 48 hours under test conditions. Therefore, all reported biological results are related to the nominal concentration of IR5878.In a limit test 4 groups of 5 first instar larvae of Chironomus riparius were exposed to IR5878 at the nominal concentration of 100 mg/L for 48 hours

Details on test conditions:

Housing: 5 animals/glass beaker of 100 mL.

Environmental conditions:
Water temperature: 19 ÷ 20°C; pH water: 7.5 ÷ 8.1; Dissolved oxygen: ≥ 8.1 mg/L
Light: 16 hours of light and 8 hours of dark.

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Details on results:

Findings
The analytically determined mean IR5878 concentrations in the analysed test media varied in the range from 104% to 106% on the nominal values. In the test media the test item was sufficiently stable during the test period of 48 hours under test conditions. Therefore, all reported biological results are related to the nominal concentration of IR5878.
In the control and in the test concentration of nominal 100 mg/L no dead test animals were observed during the test period of 48 hours.
The 48-hour NOEC and EC0 of IR5878 to the larvae of Chironomus riparius were determined to be at least 100 mg/L (the only tested concentration). The 48-hour LOEC and the 48-hour EC50 were clearly higher than 100 mg/L. These values could not be quantified due to the absence of toxicity of IR5878 up to the highest test concentration.

Validity criteria fulfilled:

yes

Conclusions:

Conclusions
The 48-hour NOEC and EC0 of IR5878 to the larvae of Chironomus riparius were determined to be at least 100 mg/L (the only tested concentration). The 48-hour LOEC and the 48-hour EC50 were clearly higher than 100 mg/L. These values could not be quantified due to the absence of toxicity of IR5878 up to the highest test concentration.

Executive summary:

Executive summary

A study was performed to evaluate the influence of IR5878 on survival of 3-4 days old larvae ofChironomus riparius. In a limit test 4 groups of 5 first instar larvae ofChironomus ripariuswere exposed to IR5878 at the nominal concentration of 100 mg/L for 48 hours. 4 additional groups of 5 animals where used as control.

The 48-hour NOEC and EC₀of IR5878 to the larvae ofChironomus ripariuswere determined to be at least 100 mg/L (the only tested concentration). The 48-hour LOEC and the 48-hour EC₅₀were clearly higher than 100 mg/L. These values could not be quantified due to the absence of toxicity of IR5878 up to the highest test concentration.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2003

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

other: OECD 203 (July 12, 1992) ; US EPA OPPTS 850.1075 (1996) ; ASTM Standard E729-88a (1994)

GLP compliance:

yes

Specific details on test material used for the study:

Test material:
IR5878
Batch number: G 009/02
Purity: 98.56 %

Analytical monitoring:

yes

Details on sampling:

Samples collected at the test initiation, at 48-hours, and at test termination had measured concentrations that ranged from 106 to 112% of nominal concentrations. When measured concentrations of the samples collected throughout the test were averaged, the mean measured test concentrations were 8.2, 16, 32, 64 and 129 mg/L, representing 109, 107, 107, 107 and 108% of nominal concentrations, respectively.

Vehicle:

yes

Remarks:

A primary stock solution was prepared by dissolving IR5878 in dilution water at a nomical concentration of 240mg/L

Details on test solutions:

Samples collected at the test initiation, at 48-hours, and at test termination had measured concentrations that ranged from 106 to 112% of nominal concentrations. When measured concentrations of the samples collected throughout the test were averaged, the mean measured test concentrations were 8.2, 16, 32, 64 and 129 mg/L, representing 109, 107, 107, 107 and 108% of nominal concentrations, respectively.

Test organisms (species):

other: Crayfish (Procambarus clarkii);

Details on test organisms:

Test animals:
Species and Strain: Red Swamp Crayfish (Procambarus clarkii)
Age: Juvenils
Body weight: 0.83 ÷ 1.6 g (body wet weight); Length: 3.2 ÷ 4.1 cm
Housing: 10 animals/test chamber.

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

128mg/L CaCO3 measured in the dilutionwater at test initiation

Test temperature:

Water temperature: 22 ± 1°C

pH:

pH water: 8.3 ÷ 8.7

Dissolved oxygen:

Dissolved oxygen: 5.2 ÷ 8.6 mg/L

Nominal and measured concentrations:

When measured concentrations of the samples collected throughout the test were averaged, the mean measured test concentrations were 8.2, 16, 32, 64 and 129 mg/L, representing 109, 107, 107, 107 and 108% of nominal concentrations, respectively.

Details on test conditions:

Environmental conditions:
Water temperature: 22 ± 1°C; pH water: 8.3 ÷ 8.7; Dissolved oxygen: 5.2 ÷ 8.6 mg/L during the test (except for five test chambers at 48-hour in which the dissolved oxygen was slightly below 60% of saturation; therefore gentle aeration was added to each chamber and dissolved oxygen levels were maintained above 60% of saturation in all test chambers until test termination)
Light: 16 hours of light and 8 hours of dark (a 30-minute transition period of low light intensity was provided at the beginning and end of the 16-hour light period to avoid sudden changes in lighting).

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 129 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

act. ingr.

Basis for effect:

mortality

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 129 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

act. ingr.

Basis for effect:

mortality

Details on results:

Measured tissue concentrations of IR5878 in the Crayfish ranged from below the LOQ up to 1.99 µg a.s./g.

Water temperatures were within the 22 +/- 1C range established for the test. Dissolved oxygen ranged from 5.2 to 8.6 during the test, except for five test chambers at 48 hours in which the dissolved oxygen was slightly below 60% of saturation. Therefore gentle aeration was added to each chamber and dissolved oxygen levels were maintained above 60% of saturation in all test chambers until test termination. Measurements of pH ranged from 8.3 to 8.7 during the test.

All cray fish in the negative control group appeared healthy and normal throughout the test. All crayfrish in the 8.2.16.32.and 129 mg/L treatment groups are appeared normal throughout the test with no mortalities or overt signs of toxicity observed. The no mortality concentration and the NOEC were 129mg/L. LC50 values at 24,48,72 and 96 hours were estimated to be greater than 129mg/L, the highest concentration tested.

Validity criteria fulfilled:

yes

Conclusions:

The 96-hour LC50 value for the Crayfish (Procambarus clarkii) exposed to IR5878 was greater than 129 mg/L, the highest concentration tested. No mortalities or effects were observed in Crayfish exposed to IR5878 at concentrations up to 129 mg/L. The 96-hour no mortality concentration and the NOEC were 129 mg/L.
Measured tissue concentrations of IR5878 in the Crayfish ranged from below the LOQ up to 1.99 µg a.s./g.

Executive summary:

Executive summary

A study was performed to determine the toxicity of IR5878 to the red swamp crayfish (Procambarus clarkii) during a 96-hour exposure period under static test conditions. Crayfish were exposed to a geometric series of five test concentrations (7.5, 15, 30, 60 and 120 mg IR5878/L) and a negative (dilution water) control.

The 96-hour LC₅₀value for the Crayfish (Procambarus clarkii) exposed to IR5878 was greater than 129 mg/L, the highest concentration tested. No mortalities or effects were observed in Crayfish exposed to IR5878 at concentrations up to 129 mg/L. The 96-hour no mortality concentration and the NOEC were 129 mg/L. Measured tissue concentrations of IR5878 in the Crayfish ranged from below the LOQ up to 1.99 µg a.s./g.

Reference 3

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Specific details on test material used for the study:

Test material:
IR5878
Batch number: FCF/T/168-00 (ex 20525/03/9)
Purity: 98.54 ± 0.51 %

Details on sampling:

Method:
A study was performed to evaluate the influence of IR5878 on the mobility respectively survival of Daphnia magna.
Groups of 20 young Daphnia magna were exposed in a static test to an aqueous test medium containing IR5878 at concentrations of 6.25, 12.5, 25, 50 and 100 mg/L. In addition, one control group of 20 animals was tested. The duration of the exposure was 48 hours.
The immobility or mortality of the Daphnia was determined after 24 and 48 hours.
Duplicate samples from the freshly prepared test media of all test concentrations and the control were taken at the start of the test. For the determination of the stability of the test item under the test conditions, respectively the maintenance of the test item concentrations during the test period, duplicate samples from the test media of all test concentrations and the control were collected at the end of the test (after 48 hours) by pouring together the contents of the test beakers of each treatment.

Details on test solutions:

Groups of 20 young Daphnia magna were exposed in a static test to an aqueous test medium containing IR5878 at concentrations of 6.25, 12.5, 25, 50 and 100 mg/L.

Test organisms (species):

Daphnia magna

Details on test organisms:

Test animals:
Species and Strain: Daphnia magna (Straus), clone 5
Age: 7 to 23.5 hours old
Housing: 10 animals/glass beaker of 250 mL.

Test type:

static

Total exposure duration:

48 h

Test temperature:

Water temperature: 21°C

pH:

pH water: 6.9 ÷ 7.9

Dissolved oxygen:

Dissolved oxygen: ≥ 9.4 mg/L

Nominal and measured concentrations:

Samples collected at beginning of the test and after 48 hours from the test concentrations of nominal 50 and 100 mg/L had measured concentrations that ranged from 89 to 99% of nominal concentrations. In the test media IR5878 was sufficiently stable during the test period of 48 hours. Therefore, all reported biological results are related to the nominal concentration.

Details on test conditions:

Test animals:
Species and Strain: Daphnia magna (Straus), clone 5
Age: 7 to 23.5 hours old
Housing: 10 animals/glass beaker of 250 mL.

Environmental conditions:
Water temperature: 21°C; pH water: 6.9 ÷ 7.9; Dissolved oxygen: ≥ 9.4 mg/L
Light: 16 hours of light and 8 hours of dark.

Key result

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

>= 50 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Key result

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 50 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Details on results:

Findings
Samples collected at beginning of the test and after 48 hours from the test concentrations of nominal 50 and 100 mg/L had measured concentrations that ranged from 89 to 99% of nominal concentrations. In the test media IR5878 was sufficiently stable during the test period of 48 hours. Therefore, all reported biological results are related to the nominal concentration.
After 24 hours in the control and in the test concentrations up to and including 50 mg/L no mortality or immobilisation of the test animals was observed. At the highest test concentration of 100 mg/L 4 Daphnia magna were immobile after 24 hours test duration (immobilisation rate of 20%). After 48 hours of exposure in the control and in the test concentrations up to and including 50 mg/L no immobility or mortality of the test animals or other signs of intoxication were observed. At the highest test concentration of 100 mg/L 4 Daphnia magna were immobile after 48 hours test duration (immobilisation rate of 20%).
The test validity criteria of OECD 202 (control immobilization ≤ 10%, no animals trapped at the surface water, and DO ≥ 2 mg/L) are fulfilled.

The results are summarised in Table 8.3-1.

 

Table 8.3-1     Influence of IR5878 on the mobility ofDaphnia magna

Concentration of IR5878	Number of       Daphnia magnatested	Number of immobilised            Daphnia magnaafter		% of immobilised                    Daphnia magnaafter
(mg/L)		24 h	48 h	24 h	48 h
0 (control)	20	0	0	0	0
6.25	20	0	0	0	0
12.5	20	0	0	0	0
25	20	0	0	0	0
50	20	0	0	0	0
100	20	4	4	20	20

 

Validity criteria fulfilled:

yes

Conclusions:

Conclusions
The 24-hour EC0 of IR5878 was 50 mg/L.
The 24-hour EC50 of IR5878 were clearly higher than 100 mg/L.
The 24-hour EC100 of IR5878 were clearly higher than 100 mg/L.
The 48-hour EC0 of IR5878 was 50 mg/L.
The 48-hour EC50 of IR5878 were clearly higher than 100 mg/L.
The 48-hour EC100 of IR5878 were clearly higher than 100 mg/L.
The NOEC of IR5878 was 50 mg/L.

Executive summary:

Executive summary

A study was performed to evaluate the influence of IR5878 on the mobility respectively survival ofDaphnia magna. Groups of 20 youngDaphnia magnawere exposed in a static test for 48 hours to an aqueous test medium containing IR5878 at concentrations of 0,12.5, 25, 50 and 100 mg/L.

The 24-hour EC₀of IR5878 was 50 mg/L. The 24-hour EC₅₀of IR5878 were clearly higher than 100 mg/L. The 24-hour EC₁₀₀of IR5878 were clearly higher than 100 mg/L. The 48-hour EC₀of IR5878 was 50 mg/L. The 48-hour EC₅₀of IR5878 were clearly higher than 100 mg/L. The 48-hour EC₁₀₀of IR5878 were clearly higher than 100 mg/L. The NOEC of IR5878 was 50 mg/L.

 

Reference 4

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: EC Directive 91/414 EEC, Annex IIA, Paragraph 8.2.4, amended by Commission Directive 96/12/EC, point 8.2.4

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Test material:
IR5878
Batch number: G 009/02
Purity: 98.56 ± 0.19 %

Details on sampling:

Mean measured concentrations at 0 hours were 76.6 to 92.7% of nominal. However concentrations at 96 hours were 89.9 to 104.6% of nominal, indicating samples were prepared accurately. Complete and homogeneous dissolution of the test item may not have occurred before sampling at 0 hours, however overall mean measured concentrations were 86.0 to 95.3% of nominal, hence results are expressed on nominal concentrations.

Details on test solutions:

Based on the results of a range finding test, the definitive test was carried out at nominal concentrations of IR5878 of 6.25, 12.5, 25, 50 and 100 mg/L. The test was carried out under static conditions over a 96 hours period. Two replicate vessels were prepared for each treatment group. Ten pond snails were added to each vessel.

Test organisms (species):

other: Lymneae stagnalis

Details on test organisms:

The test species is an aquatic gastropod mollusc common to freshwater in Europe. It is also common to aquatic environments where agricultural practices or spray drift to drainage channels may present an exposure risk to this species.

The snails used in the range finding and definitive tests were obtained from Blades Biological Cowden, Kent on 06 November 2003 and 04 February 2004 respectively.

The snails were held in conditions similar to the test from receipt until initiation of the test. This was at least 72h as required in the protocol. The snails were 16 and 18 days old at the start of the range finding test and 7 to 8 days old at the start of the definitive test.

Shell whorl numbers were recorded for each snail prior to the exposure phase of the definitive test. All snails had between 1 and 3 whorls at test initiation. The snails were provided with pond weed as food during the holding period. As juvenil snails are very fragile and adhered to the pond weed this was removed more than 24h prior to the start of the definitive test to ensure that any damaged snails could be excluded from the definitive test. This is regarded as a deviation from the protocol although it had no effect on the outcome of the study as demonstrated by the survival in the controls. Snails were not fed for the period 24h prior to either the range finder or the definitive test as per protocol and control survival was acceptable at both phases of the test.

Test type:

static

Water media type:

other: Synthetic medium prepared with deionised water and analytical grade salts (hard water formula US EPA 1975)

Total exposure duration:

96 h

Remarks on exposure duration:

Based on these results the definitive test was conducted under static conditions over a 96 h period at nomical concentrations of 6.25,12.5,25,50,100 mg/L. An untreated control was also tested.

Hardness:

Water hardness of the ASTM was determined at the start of the definitive test as 180 mg CoCO3/L

Test temperature:

Water temperature: 19.6 ÷ 20.6°C

pH:

pH water: 7.44 ÷ 8.65

Dissolved oxygen:

Dissolved oxygen: 81.9 ÷ 95.0%

Nominal and measured concentrations:

Based on the results of a range finding test, the definitive test was carried out at nominal concentrations of IR5878 of 6.25, 12.5, 25, 50 and 100 mg/L. Mean measured concentrations at 0 hours were 76.6 to 92.7% of nominal. However concentrations at 96 hours were 89.9 to 104.6% of nominal, indicating samples were prepared accurately. Complete and homogeneous dissolution of the test item may not have occurred before sampling at 0 hours, however overall mean measured concentrations were 86.0 to 95.3% of nominal, hence results are expressed on nominal concentrations.

Details on test conditions:

Test animals:
Species and Strain: Pond snail (Lymnaea stagnalis)
Age: 16 to 18 days old (start of range finding test); 7 to 8 days old (start of definitive test)
Shell whorl number: 1 to 3 at test initiation
Feeding: pond weed during holding period. Snails were not fed for the period 24 hour prior the either the range finding or the definitive test.

Environmental conditions:
Water temperature: 19.6 ÷ 20.6°C; pH water: 7.44 ÷ 8.65; Dissolved oxygen: 81.9 ÷ 95.0%
Housing: 10 pond snails for vessel

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality

Details on results:

Findings
Mean measured concentrations at 0 hours were 76.6 to 92.7% of nominal. However concentrations at 96 hours were 89.9 to 104.6% of nominal, indicating samples were prepared accurately. Complete and homogeneous dissolution of the test item may not have occurred before sampling at 0 hours, however overall mean measured concentrations were 86.0 to 95.3% of nominal, hence results are expressed on nominal concentrations.
At 96 hours after test initiation, 5% mortality was observed in the 6.25, 12.5, 25 and 50 mg/L treatment groups. This level of effect is not regarded as indicative of a test item effect, as it is below acceptable control mortality and did not show a concentration response. There was no mortality in the control or 100 mg/L treatment group.

Validity criteria fulfilled:

yes

Conclusions:

The 96-hour LC50 of IR5878 to pond snail was estimated to be > 100 mg/L and the 96-hour NOEC to be 100 mg/L, under the conditions of the test.

Executive summary:

Executive summary

A study was performed to determine the median lethal concentration (LC₅₀) of IR5878 to pond snail (Lymnaea stagnalis). Based on the results of a range finding test, the definitive test was carried out at nominal concentrations of IR5878 of12.5, 25, 50 and 100 mg/L. The test was carried out under static conditions over a 96 hours period.

The 96-hour LC₅₀of IR5878 to pond snail was estimated to be > 100 mg/L and the 96-hour NOEC to be 100 mg/L, under the conditions of the test.

Description of key information

A study was performed to evaluate the influence of IR5878 on the mobility respectively survival of Daphnia magna. Groups of 20 youngDaphnia magnawere exposed in a static test for 48 hours to an aqueous test medium containing IR5878 at concentrations of 0,12.5, 25, 50 and 100 mg/L. The 24-hour EC₀of IR5878 was 50 mg/L. The 24-hour EC₅₀of IR5878 were clearly higher than 100 mg/L. The 24-hour EC₁₀₀of IR5878 were clearly higher than 100 mg/L. The 48-hour EC₀of IR5878 was 50 mg/L. The 48-hour EC₅₀of IR5878 were clearly higher than 100 mg/L. The 48-hour EC₁₀₀of IR5878 were clearly higher than 100 mg/L. The NOEC of IR5878 was 50 mg/L.

A study was performed to evaluate the influence of IR5878 on survival of 3-4 days old larvae of Chironomus riparius. In a limit test 4 groups of 5 first instar larvae ofChironomus ripariuswere exposed to IR5878 at the nominal concentration of 100 mg/L for 48 hours. 4 additional groups of 5 animals where used as control. The 48-hour NOEC and EC₀of IR5878 to the larvae of Chironomus riparius were determined to be at least 100 mg/L (the only tested concentration). The 48-hour LOEC and the 48-hour EC₅₀were clearly higher than 100 mg/L. These values could not be quantified due to the absence of toxicity of IR5878 up to the highest test concentration.

A study was performed to determine the toxicity of IR5878 to the red swamp crayfish (Procambarus clarkii) during a 96-hour exposure period under static test conditions. Crayfish were exposed to a geometric series of five test concentrations (7.5, 15, 30, 60 and 120 mg IR5878/L) and a negative (dilution water) control. The 96-hour LC₅₀value for the Crayfish (Procambarus clarkii) exposed to IR5878 was greater than 129 mg/L, the highest concentration tested. No mortalities or effects were observed in Crayfish exposed to IR5878 at concentrations up to 129 mg/L. The 96-hour no mortality concentration and the NOEC were 129 mg/L. Measured tissue concentrations of IR5878 in the Crayfish ranged from below the LOQ up to 1.99 µg a.s./g.

A study was performed to determine the median lethal concentration (LC₅₀) of IR5878 to pond snail (Lymnaea stagnalis). Based on the results of a range finding test, the definitive test was carried out at nominal concentrations of IR5878 of12.5, 25, 50 and 100 mg/L. The test was carried out under static conditions over a 96 hours period. The 96-hour LC₅₀of IR5878 to pond snail was estimated to be > 100 mg/L and the 96-hour NOEC to be 100 mg/L, under the conditions of the test.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L