Orthosulfamuron

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 days

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Test material:
IR5878
Batch number: FCF/T/168-00 (20525/03/9)
Purity: 98.54 ± 0.51 %

Test organisms (species):

Eisenia fetida

Details on test organisms:

Test animals:
Taxonomic group: earthworms (Annelida: Oligochaeta)
Species: Eisenia fetida (Savigny 1826)
Age: adults of 8 to 9 months old, with clitellum
Body weight: 300 ÷ 600 mg
Housing: 10 individuals per normal bottling jars (1L) loosely covered by glass-lids to enable exchange of air and to minimise evaporation of the artificial soil, filled with approximately 500 g (dry weight) artificial soil
Feeding: none

Test temperature:

Temperature: 19 ÷ 20°C

pH:

pH: 5.7 ÷ 5.9 (at the beginning of the study); 5.5 ÷ 5.7 (at the end of the study)

Moisture:

Water capacity of the artificial soil: 59.02% of the dry weight
Water content: 30.5 ÷ 31.1 % corresponding to 51.2 ÷ 52.7 % of the water holding capacity (at the beginning of the study); 31.1 ÷ 32.2 % corresponding to 52.7 ÷ 54.6 % of the water holding capacity (at the end of the study)

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality

Details on results:

A study was carried out in order to determine the acute toxicity of IR5878 on the earthworm Eisenia fetida 7 and 14 days after application and estimate the LC50 of IR5878. Following a preliminary range-finding study, groups of 4 replicates (10 earthworms each) were exposed to IR5878 at concentrations of 0, 100, 178, 316, 562 and 1000 mg/kg artificial soil.
No indications for acute toxic effects of IR5878 on earthworms Eisenia fetida in artificial soil up to a concentration of 1000 mg/kg could be found. The LC50 was determined to be higher than 1000 mg/kg.

Validity criteria fulfilled:

yes

Conclusions:

No indications for acute toxic effects of IR5878 on earthworms Eisenia fetida in artificial soil up to a concentration of 1000mg/kg could be found.

Executive summary:

A study was carried out in order to determine the acute toxicity of IR5878 on the earthworm Eisenia fetida7 and 14 days after application and estimate the LC₅₀of IR5878. Following a preliminary range-finding study, groups of 4 replicates (10 earthworms each) were exposed to IR5878 at concentrations of 0, 100, 178, 316, 562 and 1000 mg/kg artificial soil.

No indications for acute toxic effects of IR5878 on earthworms Eisenia fetida in artificial soil up to a concentration of 1000 mg/kg could be found. The LC₅₀was determined to be higher than 1000 mg/kg.

Description of key information

A study was carried out in order to determine the acute toxicity of IR5878 on the earthwormEisenia fetida7 and 14 days after application and estimate the LC₅₀of IR5878. Following a preliminary range-finding study, groups of 4 replicates (10 earthworms each) were exposed to IR5878 at concentrations of 0, 100, 178, 316, 562 and 1000 mg/kg artificial soil.

No indications for acute toxic effects of IR5878 on earthwormsEisenia fetidain artificial soil up to a concentration of 1000 mg/kg could be found. The LC₅₀was determined to be higher than 1000 mg/kg.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

1 000 mg/kg soil dw