Registration Dossier
Registration Dossier
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EC number: 220-618-6 | CAS number: 2835-95-2
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
In Fish Embryo Acute Toxicity test, the 96 hour LC50 of 5-Amino-o-cresol to Danio rerio (zebrafish) was 25 mg/L (based on nominal as well as mean measured concentration).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 25 mg/L
Additional information
Fish Embryo Acute Toxicity test of 5 -Amino-o-cresol toDanio rerio(zebrafish) was determined under semi-static conditions, for 96h, following the OECD guideline 236 and the GLP. The definitive test nominal concentrations were 0 (control) 1.0, 2.2, 4.8, 11, 22 and 44 mg/L (mean measured concentration: 0.98, 2.1, 4.9, 11, 21, 46 mg/L).
The test vessels were 24-well polystyrene microtiter plates. A single plate was used for the control, each test concentration and the positive control.
Twenty wells on each plate were filled with 2 mL of the appropriate control or test media. The remaining four wells in each plate were filled with treated mains water to act as internal controls.
The % survival of eggs was 90, 90, 80, 85, 95, 75 and 0% at 0 (control), 1, 2.2, 4.8, 11, 22 and 44 mg/L test concentration respectively. Out of 20 eggs exposed, 18 eggs hatched in 0 (control) and 1 mg/L; 16 eggs hatched at 2.2 mg/L; 17 eggs hatched at 4.8 mg/L; 19 eggs hatched at 11 mg/L; 9 at 22 mg/L and 0 at 44 mg/L.For the reference substance, 3,4-dichloroaniline, the observed mortality was 100% which was in excess of the minimum 30% validity criteria.
Hence, the 96 hour LC50 of 5 -Amino-o-cresol toDanio rerio(zebrafish) was 25 mg/L (based on nominal as well as mean measured concentration).
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