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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro cytogenicity / chromosome aberration study in mammalian cells
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Peer-reviewed researh journal.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1991

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
Version / remarks:
Equivalent to guideline in terms of methods and cell-lines used
GLP compliance:
not specified
Type of assay:
other: in vitro mammalian chromosome aberration test (migrated information)

Test material

Constituent 1
Chemical structure
Reference substance name:
7-methyl-3-methyleneocta-1,6-diene
EC Number:
204-622-5
EC Name:
7-methyl-3-methyleneocta-1,6-diene
Cas Number:
123-35-3
Molecular formula:
C10H16
IUPAC Name:
7-methyl-3-methyleneocta-1,6-diene
Specific details on test material used for the study:
Major component of hop oil and other related plant oils used in foods, cosmetics and medicine

Method

Species / strain
Species / strain / cell type:
lymphocytes: Human
Details on mammalian cell type (if applicable):
Peripheral blood samples were obtained from two healthy probands (one male, one female, both non- smokers).
Metabolic activation:
with and without
Metabolic activation system:
Rat liver homogenate induced with Aroclor-1254
Test concentrations with justification for top dose:
Concentrations between 100 and 1,000 ug/ml were tested.
It is reported that the maximum concentration was limited by the solublity in the test media

Results and discussion

Test results
Species / strain:
lymphocytes: Human
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity, but tested up to precipitating concentrations
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified

Applicant's summary and conclusion

Conclusions:
It was concluded in this research that there was no evidence of effects on chromosomes.
It is also worth noting that the authors performed further review work, noting no mutagenic effects of this plant oil.