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EC number: 600-065-0 | CAS number: 1003218-40-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July 16, 2013 - December 31, 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- The short-term toxicity to fish study was performed for a registration in a non-EU country.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)
- Version / remarks:
- 1996
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- The samples were taken in duplicate from each concentration during limit test at 0, 24, 48, 72 and 96 h.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: For the limit test WAF solutions with a nominal concentration of 100 mg/L was prepared. The Aqueous test substance mixture was stirred for 24 h at 23-25°C. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebra fish
- Source: Guangzhou Hongfa Aquatic Animals Culture Company, China
- Length at study initiation: mean 2.30 cm
- Weight at study initiation: mean 0.239 g
ACCLIMATION
- Acclimation period: Feeding fry held for 28 days supplied with a continuous flow of aerated water before test. Fish to be used for test was held for 7 days under test conditions.
- Acclimation conditions: same as test
- Type and amount of food during acclimation: crude protein >36.0%, crude fat >2.0%, crude fibre <3.0%, crude ash <12%, moisture <10.0% - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 122 - 124 mg/L CaCO3
- Test temperature:
- 22.9 - 23.2°C
- pH:
- 7.30 - 7.45
- Dissolved oxygen:
- 81 - 87% of saturation
- Nominal and measured concentrations:
- Nominal concentration: 100 mg/L (WAF)
Mean measured concentration: 1.85 mg/L - Details on test conditions:
- TEST SYSTEM
- Aeration: no
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.7 - 0.8 g fish/L
TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: dechlorinated tap water
OTHER TEST CONDITIONS
- Photoperiod: 16 h light/ 8 h dark
- Light intensity: 1000 - 1500 lux
EFFECT PARAMETERS MEASURED:
mortality, inactivity, abnormal swimming pattern, other abnormal behaviour recorded at 24, 48, 72 and 96 h
measurements of pH, dissolved oxygen and temperature were carried out and recorded daily.
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 1.0, 10.0, 100 mg/L (no mortality) - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: water accomodated fraction (WAF)
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1.85 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mortality (fish)
- Details on results:
- During the test period, the pH values of the control media and test media were between 7.30 and 7.45, and the Dissolved Oxygen (DO) values varied from 81% to 87% of the air saturation at the test temperature, and the total hardness was in the range of 122 to 124 mg (CaC03)/L. During the test, the temperature of the test media were maintained in the range of 22.9 °C to 23.2 °C, and all fishes in the control group were normal. So the study met the acceptability criteria prescribed by the protocol (pH: 6.0 -8.5; dissolved oxygen concentration: > 60% of the air saturation value; total hardness: 10-250 mg (CaC03)/L; temperature: (23±2) °C; Therefore the test was considered valid.
- Results with reference substance (positive control):
- 24h-LC50: 269 mg/L (potassium dichromate), acceptablility criteria: 24 h-LC50 of K2Cr2Ov in the range of 200 to 400 mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of the test item to Zebra fish (Brachydanio rerio) was determined in a study according to OECD 203. For the limit test a WAF (water accomodated fraction) of 100 mg/L was prepared, the measured concentration was 1.85 mg/L. No toxic effects were observed within 96 h. Therefore, the LC50 is determined to be >100 mg/L (WAF) and 1.85 mg/L (measured concentration).
- Executive summary:
The test item was tested in a GLP study according to OECD 203. A range-finding test and then a Limit Test were performed respectively. Nominal concentrations of 1 - 100 mg/L were used in the range-finding test, while the nominal concentration of 100 mg/L WAF was used in the Limit Test. In the range-finding test, 5 fishes per treatment and no replicates were used and 7 fish per group were used in the Limit Test. The test fishes were exposed for 96 hours to the test solution. During the test period, the pH values of the control media and test media were between 7.3 - 7.45, the dissolved oxygen values varied from 81 – 87 % of the air saturation at the test temperature, and the total hardness was in the range of 122 – 124 mg CaCO3/L. During the test, the temperature of the test media were maintained in the range of 22.9 - 23.2°C and all fish in the control group were normal. With the same conditions, K2Cr2O7 was used as the positive control substance, and the resulting 24 h-LC50 was 269 mg/L. So the study met the acceptability criteria prescribed by the test protocol and the test was considered as valid. During the test period, all fish in the control group and treated group were alive and appeared normal. The results show that under valid static test conditions, the 96 h-LC50 of the test item is greater than the nominal concentration of 100 mg/L WAFs (mean measured concentration 1.85 mg/L), while the maximum concentration causing no mortality (96 h-LC0) is equal to the nominal concentration of 100 mg/L WAFs (measured concentration 1.85 mg/L). In this study no toxic effects were observed within 96 h. Therefore, the LC50 is determined to be >100 mg/L (WAF) and 1.85 mg/L (measured concentration).
Reference
Description of key information
The acute toxicity of the test item to Zebra fish (Brachydanio rerio) was determined in a study according to OECD 203. For the limit test a WAF (water accomodated fraction) of 100 mg/L was prepared, the measured concentration was 1.85 mg/L. No toxic effects were observed within 96 h. Therefore, the LC50 is determined to be >100 mg/L (WAF) and 1.85 mg/L (measured concentration) (reference 6.1.1 -1).
Key value for chemical safety assessment
Additional information
The test item was tested in a GLP study according to OECD 203. A range-finding test and then a Limit Test were performed respectively. Nominal concentrations of 1 - 100 mg/L were used in the range-finding test, while the nominal concentration of 100 mg/L WAF was used in the Limit Test. In the range-finding test, 5 fishes per treatment and no replicates were used and 7 fish per group were used in the Limit Test. The test fishes were exposed for 96 hours to the test solution. During the test period, the pH values of the control media and test media were between 7.3 - 7.45, the dissolved oxygen values varied from 81 – 87 % of the air saturation at the test temperature, and the total hardness was in the range of 122 – 124 mg CaCO3/L. During the test, the temperature of the test media were maintained in the range of 22.9 - 23.2°C and all fish in the control group were normal. With the same conditions, K2Cr2O7 was used as the positive control substance, and the resulting 24 h-LC50 was 269 mg/L. So the study met the acceptability criteria prescribed by the test protocol and the test was considered as valid. During the test period, all fish in the control group and treated group were alive and appeared normal. The results show that under valid static test conditions, the 96 h-LC50 of the test item is greater than the nominal concentration of 100 mg/L WAFs (mean measured concentration 1.85 mg/L), while the maximum concentration causing no mortality (96 h-LC0) is equal to the nominal concentration of 100 mg/L WAFs (measured concentration 1.85 mg/L). In this study no toxic effects were observed within 96 h. Therefore, the LC50 is determined to be >100 mg/L (WAF) and 1.85 mg/L (measured concentration) (reference 6.1.1 -1).
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