Amines, C12-14-tert-alkyl, bis[2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]benzoato(2-)]chromate(1-)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1987-10-12 to 1987-10-19

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1984

Deviations:

no

GLP compliance:

no

Remarks:

However, Quality Assurance Statement available

Test material

Test material information

Constituent 1

Reference substance name:

Amines, C12-14-tert-alkyl, bis[2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]benzoato(2-)]chromate(1-)

EC Number:

287-007-4

EC Name:

Amines, C12-14-tert-alkyl, bis[2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]benzoato(2-)]chromate(1-)

Cas Number:

85408-46-4

Molecular formula:

C17H12N4O3 . C17H12N4O3 . Cr. C12H28N to C14H32N

IUPAC Name:

Amines, C12-14-tert-alkyl, bis[2-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]benzoato(2-)]chromate(1-)

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: samples were taken from all concentrations plus the blank and the vehicle control to measure oxygen, pH and temperature at the time points 0h, 24 h, 48 h, 72 h and 96 h

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Calculated amounts of test substance to produce the desired test concentrations were given into the water and homogeneously distributed. The fishes were then transfered into the aquaria.
- Controls: Blank: water; vehicle: 95 mg DMF per liter water
- Evidence of undissolved material: The test substance appeared homogeneously distributed in all test concentrations.
- Stock solution: 1.00 g of the test substance was dissolved in and made up to 10 ml with DMF
- Concentration of vehicle in test medium: 95 mg DMF per liter water in the concentration used for the highest test concentration

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Strain: Brachydanio rerio
- Source: West-Aquarium, 3422 Bad Lauterberg, Germany
- Length at study initiation: 23 mm (18-25 mm)
- Weight at study initiation: 0.09 g (0.06-0.12 g)
- Feeding during test: none

ACCLIMATION
- Acclimation period: 40 days
- Acclimation conditions: same as test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

none

Test conditions

Hardness:

180 mg CaCO3/L

Test temperature:

22+/- 1 °C

pH:

8-8.3

Dissolved oxygen:

no data

Salinity:

no data

Nominal and measured concentrations:

test concentrations (nominal): 1.0, 1.8, 3.2, 5.0, 10.0, mg/L
measured (after 96 h): 1.2, 2.1, 3.2, 5.9, 10.0 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: aquaria
- Material, size, headspace, fill volume: Glass aquaria of 20 liters filled with 15 liters (36/22/25 cm)
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.06 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water (carbon filter)


OTHER TEST CONDITIONS
- Photoperiod: 16 h light /8 h dark


TEST CONCENTRATIONS
- Test concentrations: 1.0 1.8 3.2 5.0 10.0 mg/L (nominal)

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

5.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: At the concentration of 10 mg/L severe symptoms cocnerning swimming behaviour, loss of equilibrium and respiratory functions were observed after 96 h of exposure. At the concentration of 5.8 mg/L moderate effects concerning swimming behaviour and loss of equilibrium and slight efffects on respiratory function were observed after 96 h.
- Mortality of control: none
- Any observations that might cause a difference between measured and nominal values: The test substance appeared homogeneously distributed in all test concentrations.

Reported statistics and error estimates:

The LC50 values were calculated according to J.BERKSON , JASA 48 (1953), 569-599. LC50 (96 h) was graphically determined on gausso-logarithmic probability paper.

Any other information on results incl. tables

Sublethal observations / clinical signs:

The test substance appeared homogeneously distributed in all test concentrations.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test substance is acutely toxic to fish.