Fatty acids, C9-13-neo-

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted accorrding to accepted OECD protocols and GLP

Justification for type of information:

See read across justification

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

water accommodated fraction: loading rated of 0.3 - 30 mg/L. Stirred for 69-73 hours

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Healthy O. mykiss fingerlings were obtained from Zeals Trout Farm, Zeals, Wiltshire, and acclimated to the test conditions for more than nine days before use. A sample of seven of the fish used in the test had a mean length of 4.6 cm and a mean weight of 0.76 g.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

276-292mg/l as CaCO3

Test temperature:

15.4-16.3 C

pH:

7.1-7.6

Dissolved oxygen:

8.6-9.1 mg/l

Details on test conditions:

A sealed 96 h semi-static toxicity test was carried out with daily renewal of the test media. The test vessels were glass aspirators which were completely filled (11 liters) with WAF of VERSATIC 1019. Seven O. mykiss were placed in each vessel. The vessels were then sealed with glass plated ensuring that all air was excluded. The fish were not fed during the test.

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

10 - 30 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

3 - 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

3 - 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

3 - 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Conclusions:

The 96 h LL50 of VERSATIC 1019 as determined by a OECD 203 Acute fish toxicity test is between 3 and 10 mg/l.

Executive summary:

The acute toxicity to O. mykiss of water accommodated fractions of VERSATIC 1019 was determined in a 96 h semi-static toxicity test with daily renewal of the test media. The test was conducted in seled test vessels. The 24, 48, 72 and h LL50 values (loading rates producing WAF resulting in mortality of 50% of the fish after 24, 48, 72 and 96 h exposure) were within the ranges 10 -30, 3 -10, 3 -10 and 3 -10 mg/l respectively.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Justification for type of information:

See read across justification

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Measuring times: temperature hourly, pH and dissolved oxygen of the fresh media at 0, 24, 48, 72 and 96 h and the old media at 24, 48, 72, and 96 h. For analytical verification of exposure concentration the sampling times were: 0, 24, 48, 72, and 96 h.

Vehicle:

no

Details on test solutions:

For the preparation of stock solution 100 mg/L of test substance was added to test medium and stirred for 48 hours in sealed aspirators. After standing for 1-1.5 hours, the stock solutions were drawn off (WAF, water accomodated fraction method) to make the different concentrations. The final loading rate concentrations of Neononanoic acid WAF were: 0, 100, 220, 460, and 1000 mg/L.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

The supplier was Brow Well Fisheries, Ltd, Hebden, Yorkshire (UK). Fish Age/size/weight were fingerlings of 4.1-4.7 cm length and weighing 0.58-0.98 g. Feed (pretreatment) was provided unit 24 h prior to the test. There was no feed provision during test. The acclimation period was more than 12 days.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None

Hardness:

150-168 mg CaCO3/L

Test temperature:

14.5-15.9°C

pH:

5.1-9.1 (including non pH adjusted)

Dissolved oxygen:

6.4-9.3 mg/L

Salinity:

Freshwater

Nominal and measured concentrations:

The nominal concetrations of neononanoic acid WAF were: 0, 100, 220, 460, and 1000 mg/L. Measured concentrations of Neononanoic acid were: (mg/L): <0.5, 97, 173, 352, and 777.

Details on test conditions:

The 460 and 1000 mg/L loading rate concentrations had lower pH and a concurrent pH adjusted test concentration was run. The fish were exposed to WAF of neononanoic acid at 0, 10, 22, 46, and 100% of stock solution with daily renewal (Semistatic). Exposure vessels were 10 L glass bottles sealed with a glass plate and filled with 10 L of medium. There were 7 fish per replicate, 1 replicate/treatment condition. The study was conducted for 96 hours without aeration. The photoperiod: was 16 h light, 8 h dark. During exposure the fish were observed at 3, 24, 48, 72 and 96 h for mortality and sublethal signs.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 460 - < 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

loading rate

Basis for effect:

mortality (fish)

Remarks on result:

other: WAF

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

460 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

loading rate

Basis for effect:

mortality (fish)

Remarks on result:

other: WAF

Details on results:

Fish mortality at the measured concentrations of neononanoic acid at 96 h (pH adjusted) of (mg/L): <0.5, 97, 173, 352, and 777, were, 0/7, 0/7, 0/7, 0/7, and 7/7, respectively.

Conclusions:

The rainbow trout 96-h LC50 for WAF of Neononanoic was >460 (pH adjusted) mg/L. The NOEC for mortality was 460 mg/L (pH adjusted).

Executive summary:

The results of an O.E.C.D. 203 Testing Guideline study with rainbow trout demonstrated that the 96 - hr LC50 value for Neononanoic acid was > 460 mg/l with a NOEC for mortality of 460 mg/L.

Reference 3

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Justification for type of information:

See read across justification

Reason / purpose for cross-reference:

read-across source

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

152-168 mg CaCO3/L

Test temperature:

14.4-15.6 deg C

pH:

8-8.7

Dissolved oxygen:

6.6-9.3

Nominal and measured concentrations:

Nominal: 30, 100, 300, 1000 mg/L
Measured: 25, 85, 305, 176

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

> 100 - < 300 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

The 96-h LL50 of Versatic 10 to rainbow trout was >100 mg/L

Executive summary:

The 96-h LL50 of Versatic 10 to rainbow trout was >100 mg/L

Additional information

The acute toxicity of Versatic 10, as measured by mortality to rainbow trout (Oncorrhynchus mykis) was evaluated in freshwater. Under the conditions of this study, the 96 -h LL50 was >100 mg/L based on measured concentrations of the test substance in water.

The acute toxicity to O. mykiss of water accommodated fractions of VERSATIC 1019 was determined in a 96 h semi-static toxicity test with daily renewal of the test media. The test was conducted in seled test vessels. The 24, 48, 72 and h LL50 values (loading rates producing WAF resulting in mortality of 50% of the fish after 24, 48, 72 and 96 h exposure) were within the ranges 10 -30, 3 -10, 3 -10 and 3 -10 mg/l respectively.

The results of an O.E.C.D. 203 Testing Guideline study with rainbow trout demonstrated that the 96 - hr LC50 value for Neononanoic acid was > 460 mg/l with a NOEC for mortality of 460 mg/L.