4-[difluoro(3,4,5-trifluorophenoxy)methyl]-2',3,5-trifluoro-4''-propyl-1,1':4',1''-terphenyl

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)

Principles of method if other than guideline:

solubiliser was applied to achieve the final concentrations above the water solublity limit

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-{4-[difluoro(3,4,5-trifluorophenoxy)methyl]-3,5-difluorophenyl}-3-fluoro-4'-propyl-1,1'-biphenyl

EC Number:

619-490-8

Cas Number:

303186-36-9

Molecular formula:

C27H22F8O2

IUPAC Name:

4-{4-[difluoro(3,4,5-trifluorophenoxy)methyl]-3,5-difluorophenyl}-3-fluoro-4'-propyl-1,1'-biphenyl

Test material form:

solid: bulk

Radiolabelling:

no

Test solutions

Vehicle:

yes

Remarks:

HCO-40

Details on preparation of test solutions, spiked fish food or sediment:

HCO-40 was used as solubilizer

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: carp
Length at study initiation: 8.3 cm
- Weight at study initiation: 6.4 g

Study design

Route of exposure:

aqueous

Justification for method:

other: most valid guideline in 2008

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

28 d

Test conditions

Test temperature:

25 +-2 Grad

Details on test conditions:

according to Guideline OECD 305 (test year 2008)
RANGE-FINDING / PRELIMINARY STUDY - Test concentrations : LC50(96h) > 100 mg/L, Rice fish (Oryzias latipes)

After acclimatisation period, the fish (Cyprinus carpio) were transferred into the test media under flow through conditions. Exposure concentrations were analytically verified at days 4, 7, 14, 21, and 28. The analytical values show that the nominal concentrations of 0.5 and 0.05 mg/L were maintained at about 100% using solubiliser, respectively. At days 4, 7, 14, 21 and 28 two fish each were removed from each test group. Body weight was determined. The fish were homogenised and extracted using organic solvents. The extract was analysed with HPLC. Due to the low bioaccumulation potential, no depuration phase was required.

Nominal and measured concentrations:

- Nominal concentrations 1st Concentration area 0.001 mg/l and 2nd Concentration area 0,0001 mg/L

nominal concentration 0.001 mg/L: HCO-40: 1 mg/L
analytically measured concentrations:

day 4: 0.000991 mg/l
day 7: 0.00100 mg/L;
day 14: 0.00102 mg/L
day 21: 0.000994 mg/L
day 28: 0.000994 mg/L

nominal concentration 0.0001 mg/L, HCO-40: 0.1 mg/L
analytically measured concentrations:

day 4: 0.000101 mg/L
day 7: 0.0000999 mg/L
day 14: 0.000100 mg/L
day 21: 0.000100 mg/L
day 28: 0.000100 mg/L

Results and discussion

Lipid content

Lipid content:

ca. 5 %

Time point:

other: literature value

Remarks on result:

other: E. G. Bligh and W. J. Dyer, Can. J. Biochem, Physiol., 37, 911 (1959)

Bioaccumulation factor

Bioaccumulation factor 1

Key result

Conc. / dose:

0.001 mg/L

Type:

BCF

Value:

63 dimensionless

Bioaccumulation factor 2

Key result

Conc. / dose:

0 mg/L

Type:

BCF

Value:

53 dimensionless

Details on kinetic parameters:

Steady state was reached within 14 days
Due to the low uptake, no depuration phase was necessary

Details on results:

- Mortality of test organisms: no effects
- Behavioural abnormalities: no effects
- Observations on body length and weight: no effects
- Other biological observations: no effects
- Bound residues forming a plateau: no
- Mortality and/or behavioural abnormalities of control: no
- Loss of test substance during test period: no
- Results with vehicle control: no effects

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 64 and 59 (mean 61).