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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Oct 13-16, 2008
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Report date:
2008

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
Deviations:
no
Principles of method if other than guideline:
None
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
4-{4-[difluoro(3,4,5-trifluorophenoxy)methyl]-3,5-difluorophenyl}-3-fluoro-4'-propyl-1,1'-biphenyl
EC Number:
619-490-8
Cas Number:
303186-36-9
Molecular formula:
C27H22F8O2
IUPAC Name:
4-{4-[difluoro(3,4,5-trifluorophenoxy)methyl]-3,5-difluorophenyl}-3-fluoro-4'-propyl-1,1'-biphenyl
Test material form:
solid: bulk

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
The test medium (reconstituted water and test material) was freshly prepared. The calibrated flask with test material and vehicle, reconstituted water, was treated in an ultrasound bath for 1 hour. Subsequently, the preparation was stirred with a magnetic stirrer for further 23 hours. Afterwards, the preparation was filtered through a filter funnel with a pore size of 10-16 µm. The filtrate was used for the study.

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
The test system used for this study was a green unicellular algae species, Desmodesmus subspicatus, strain No. SAG 86.81, supplied by the "Sammlung von Algenkulturen, Pflanzenphysiologisches Institut der Universitat Gottingen". This strain is recommended by international guidelines. At the start of the experimental part, an algae stock suspension was prepared from the preculture containing about 10,000 algae cells/mL.

Study design

Test type:
static
Water media type:
not specified
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Hardness:
250 mg CaCO3
Test temperature:
22-23°C
pH:
8+/-1
Dissolved oxygen:
80 %
Salinity:
for details see composition of reconstituted water "details on test conditions"
Nominal and measured concentrations:
Nominal concentration of 100 mg/L (limit test); due to very low water solubility and the fact that the study was a limit test, no further analysis was initiated.
Details on test conditions:
The test medium (reconstituted water and test material) was freshly prepared. The light intensity was approximately 7500 to 9500 Lux. The experimental part was started by inoculation of algae to the different groups so that the flasks contained about 10,000 cells/mL. The algae were taken from an exponentially growing pre-culture (growth rate 120.7) which was set up 3 days prior to the experimental part under the same conditions as in the final study.

Details on composition of the reconstituted water:

Macro-nutrients:
50.00 mg/L NaHCO3
18.00 mg/L CaCl2 x 2 H2O
15.00 mg/L NH4Cl
15.00 mg/L MgSO4 x 7 H2O
12.00 mg/L MgCl2 x 6 H2O
1.60 mg/L KH2PO4

Trace elements:
100.00 µg/L Na2EDTA x 2 H2O
80.00 µg/L FeCl3 x 6 H2O
415.00 µg/L MnCl2 x 4 H2O
185.00 µg/L H3BO3
7.00 µg/L Na2MoO4 x 2 H2O
3.00 µg/L ZnCl2
1.50 µg/L CoCl2 x 6 H2O
0.01 µg/L CuCl2 x 2 H2O

The pH of the reconstituted water after aeration is approximately 8.
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Results with reference substance (positive control):
Regularly, a positive control test to check the test system is carried out with potassium dichromate (no results provided in the study report).

Any other information on results incl. tables

The test material concentration in the aqueous medium was not quantified at the start and at the end of this study as a very low water solubility was calculated for this test item (ca. 0.059 µg/L). Therefore, the development of an analytical method with a sufficiently low detection and quantification limit would be very time and cost intensive. Due to the absence of any adverse effects at the saturation concentration, the study was performed without analytical concentration verification.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
An aqueous preparation of 100 mg/L (nominal) of the test item revealed no toxic effect in the test system. Therefore, the 72 hours EC50 could not be determined, because it exceeded the maximum solubility of the test material in reconstituted water (EC50 > 0.00021 mg/L).
Executive summary:

Purpose

The purpose of this assay was to identify the aquatic toxicity potential of the test item in algea to provide a rational basis for hazard estimation for the test item in aquatic environments.

Study design

The influence of the test material on the growth and growth rate of the green algae species Desmodesmus subspicatus was tested. The study design included one control and one test material group with three replicates, each containing about 100 rnL reconstituted water or test medium and 10,000 cells/mL at the start of the experimental phase. The algae were exposed to a filtrate of nominal 100 mg/L and the study was performed as a limit test in an open static test system. The growth was calculated after 24, 48, and 72 hours exposure in the test medium.

Results

The test material concentration in the aqueous medium was not quantified at the start and the end of this study due to the low water solubility (< 0.00021 mg/L). Because of the low water solubility, the compound cannot be detected with standard analytical methods. The development of an analytical method with a sufficiently low detection and quantification limit is complex. Due to the absence of any adverse effects at the saturation concentration, the study was performed without analytical concentration verification.

After exposure of Desmodesmus subspicatus for 72 hours the following results were obtained.

72 h EbC50 > 0.00021 mg/L (nominal > 100 mg/L)

72 h ErC50 > 0.00021 mg/L (nominal > 100 mg/L)

NOEC > 0.00021 mg/L (nominal > 100 mg/L)

Conclusions

An aqueous preparation of 100 mg/L (nominal) of the test item revealed no toxic effect in the test system. Therefore, the 72 hours EC50 could not be determined, because it exceeded the maximum solubility of the test material in reconstituted water (EC50 > 0.00021 mg/L).