Dodecyltrimethylammonium chloride

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

12th to 14th June 1984

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

other: "Methods for acute toxicity tests with fish, macroinvertebrates, and amphibians" U.S. EPA, 1975

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

reference material: x0296.01

Material description: clear, colorless liquid, 28% active ingredient

Analytical monitoring:

not specified

Details on sampling:

Test concentrations: 0.39 ; 0.65 ; 1.1 ; 1.8 ; 3.0 ; 5.0 mg/L
Fifteen daphnids, < 24 hours old, were impartially distributed to each concentration (five daphnids per replicate) within

Vehicle:

no

Details on test solutions:

A clear, colorless stock solution of 1.0 mg/mL was formulated by diluting 0.179 grams of X0296.01 with distilled water to volume in a 50-mL volumetric flask Test solution were formulated by adding the appropriate volumes of the stock solution to dilution water to total 1000 mL. Each solution was mixed on a magnetic strirrer for one minute and then divided into three beakers to provide replicate exposure treatments each containing 200 mL. The excess 400 mL of test solution was discarded. The test solution depth was 6.2 centimeters (cm) with a surface area of 33 cm2. Three control beakers containing the same dilution water and maintening under the same conditions as the exposure concentrations, but containing no X0296.01, were established.
Mortailities in replicate test solutions were recorded at 24 and 48 hours of exposure. Biological observations and observations of the physical characteristics of each replicate test solution were also made and recorded at 0, 24, and 48 hours. The pH's and dissolved oxygen concentrations were measured at 0 and 48 hours in one replicate vessel of each test concentration and the control. Temperature was measured in one replicate vessel of the control at 0, 24 and 48 hours of exposure.

Test organisms (species):

Daphnia magna

Details on test organisms:

The daphnids used in this toxicity test were obtained from laboratory stocks cultured at Springborn Bionomics, Inc., Wareham, Massachusetts. The culture water was prepared by fortifying well water based on the formula for hard water (U.S. EPA, 1975) and filtering it through carbon and Amberlite XAD-7 resin colums to remove any potential organic contaminants. this water had total hardness and alkalinity ranges as calcium carbonate (CaCO3) of 160-180 mg/L and 110-130 mg/L, respectively, a pH range of 7.9-8.3, a temperature of 20 +/- 1°C, a dissolved oxygen concentration of greater than 60% of saturation and a specific conductance range of 400-600 micromhos per centimeter (µmhos/cm). The daphnid culture area recieved a regulated photoperiod of 16 hours of light and eight hours darkness. Light at an intensity of 5-10 hectolux at the culture solutions' surface was provided by a combination of Sylvania Growlux(R) and Cool White(R) fluorescent bulbs. Daphnids were fed a solution of green algae (Ankistrodesmus sp.) and yeast suspension once daily. The air temperature was controlled to maintain the culture solutions at 20 +/- 1°C.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

not applicable

Hardness:

hardness and alkalinity ranges as calcium carbonate (CaCO3) of 160-180 mg/L and 110-130 mg/L, respectively.

Test temperature:

Test temperature: 20 +/- 1°C

pH:

pH range of 7.9-8.3

Dissolved oxygen:

Dissolved oxygen concentration of greater than 60% of saturation

Salinity:

NA

Conductivity:

specific conductance range of 400-600 micromhos per centimeter (µmhos/cm).

Nominal and measured concentrations:

Nominal concentrations: 0.39 ; 0.65 ; 1.1 ; 1.8 ; 3.0 ; 5.0 mg/L

Measured concentrations: results are reported as nominal concentrations of X0296.01 based on 28% active ingredient.

Details on test conditions:

Test Vessels: 250 mL
Type: Open
Aeration: Slighty aerated
Number of organisms per vessels: 5
Number of vessels per concentrations (replicate): 3
Photoperiod: light/dark cycle 16/8 hours
Source/Preparation of dilution water: prepared as described in OECD 202 Test Guideline

Reference substance (positive control):

yes

Remarks:

A sodium lauryl sulfate reference test was conducted with the daphnid test population from 15-17 May 1984

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

ca. 0.46 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

The concentrations tested, corresponding mortalities and observations made during the test. The 48-hour LC50 for Daphnia magna exposed to X0296.01 was calculated by nonlinear interpolation to be 0.46 mg/L with a 95% confidence interval of 0.39-0.65 mg/L estimated by binomial probability method. Table below in the section "Any other information on results incl. tables".

Results with reference substance (positive control):

A sodium lauryl sulfate reference test was conducted with the daphnid test population from 15-17 May 1984. The 48-hour LC50 and 95% confidence interval was 6.2(5.0-7.5) mg/L (Reference test log).

Concentrations tested, corresponding cumulative mortalities and observations made during the 48 -hour exposure of daphnids (Daphnia magna) to X0296.01.

	Cumulative Mortality (%)
	24 -hour				48 -hour
Nominal concentration (mg/L)	A	B	C	x	A	B	C	x
5.0	100	100	100	100	100	100	100	100
3.0	100	100	100	100	100	100	100	100
1.8	100	100	100	100	100	100	100	100
1.1	60	80	60	67a	100	100	100	100
0.65	0	0	0	0a	100	100	100	100
0.39	0	0	0	0	20	20	20	20a
control	0	0	0	0	0	0	0	0

(a): Sixty-seven percent or more of the surviving daphnids were lethargic and on the bottom of the test vessel.

Validity criteria fulfilled:

yes

Conclusions:

Results arte reported as nominal concentrations of X0296.01 based on 28% active ingredient. The 48-hour LC50 was calculated by nonlinear interpolation to be 0.46 mg/L with a 95% confidence interval of 0.39-0.65 mg/L estimated by binomial probability.

Executive summary:

Introduction:

The purpose of this study was to estimate the acute toxicity of X0296.01 to daphnids (Daphnia magna) under static conditions. A 48 -hour definitive test was conducted from 12 -14 June 1984, at the Aquatic Toxicology Laboratory of Springborn Bionomics, Inc., Wareham, Massachusetts. All raw data generated are stored at the above location.

Materials and methods:

Procedure used in this acute toxicity study followed those described in "Methods for acute toxicity tests with fish, macro-invertebrates, and amphibians" (U.S. EPA, 1975) and the protocol entitled "Static acute Frashwater Invertebrate Toxicity Study of X0296.01" (M.A. Lewis, Principal Investigator, 26 April 1984).

Results:

The concentrations tested, corresponding mortalities and observations made during the test. The 48 -hour LC50 for D. magna exposed to X0296.01 wazs calculated by nonlinear interpolation to be 0.46 mg/L with a 95% confidence interval of 0.39 -0.65 mg/L estimated by nominal probability method.

Description of key information

Acute Toxicity of X0296.01 to Daphnia magna

Report #BW-84 -7 -1594

Study #1011 -0584 -6110 -110

Material: X0296.01

Description material: clear, colorless liquid, 28% active ingredient

Test date: 12 -14 June 1984

Species: Daphnia magna / Age: < 24 hours old / source: Bionomics culture facility

Dilution Water: fortified well water

Total hardness as CaCO3: 160mg/L

Total alkalinity as CaCO3: 120mg/L

pH: 8.3

Specific conductivity: 500µmhos/cm

Test temperature: 20 -21°C

Method of test material addition: stock solution, 1.0mg W0296.01/mL distilled water

Test concentrations: 0.39 ; 0.65 ; 1.1 ; 1.8 ; 3.0 ; 5.0 mg/L

Test vessels: 250mL

Number of organisms per vessels: 5

Number of vessels per concentrations (replicate): 3

photoperiod: light/dark cycle 16/8 hours

Source preparation of dilution water: prepared as described in OECD 202 test Guideline.

Test reference: A sodium lauryl sulfate reference test was conducted with the daphnid test population from 15 -17 May 1984. The 48 -hour LC50 and 95% confidence interval was 6.2(5.0 -7.5) mg/L (Reference test log).

Results: The concentrations tested, corresponding mortalities and observations made during the test. The 48 -hour LC50 for D. magna exposed to X0296.01 was calculated by nonlinear interpolation to be 0.46 mg/L with a 95% confidence interval of 0.39 -0.65 mg/L estimated by binimial probability method.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.46 mg/L