Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Experimental start date 21 April 2017 Experimental completion date 05 May 2017
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
OECD Guideline 203 (Fish, Acute Toxicity Test)
according to guideline
EU Method C.1 (Acute Toxicity for Fish)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
Details on test solutions:
Analysis of the freshly prepared bulk test preparations at 0 and 72 hours showed measured test concentrations to be 90 and 88mg/L. There was no significant change in the measured concentrations of the old or expired test media at 24 and 96 hours and so the results are based on nominal concentration.
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
The test was carried out using juvenile rainbow trout. The stock fish were fed commercial trout pellets which was discontinued approximaelty 22.5 hours prior to the start of the definitive test. There was no mortality in the 7 days prior to the start of the test and the fish had a mean standard length of 5.5cm ( sd = 0.40) and a mean weight of 1.4g (sd = 0.4) at the end of the definitive test. Based on the mean weight vlaue this gave a loading rate of 0.48g bodyweight/liter ( static volume).
Test type:
Water media type:
Limit test:
Total exposure duration:
96 h
Test temperature:
Fresh media control pH range 7.6 - 8.1
Nominal concentration 100 pH range 7.9 -8.2
Dissolved oxygen:
9.5mg O2/L
Nominal and measured concentrations:
Nominal concentration of 100mg/L was used.
Details on test conditions:
At the start of the test seven fish were placed in each test vessel at random, in the test preparations. The vessels were covered to reduce evaporation and maintained at 14-16°C with a photoperiod of 16 hours light and 8 hours darkness with a 20 minute dawn and dusk transition. The water temperature, pH and dissolved oxygen concentrations were recorded daily throughout the test, The measurements at 0 hours and after each test media renewal at 24, 48 and 72 hours, represent those of the freshly prepared test preparations. The pH and dissoved oxygen concentrationswere measured using a Hach flex handheld meter, the temperature was measured using a digital thermometer.
Key result
96 h
Dose descriptor:
Effect conc.:
> 100 mg/L
Nominal / measured:
Conc. based on:
test mat.
Details on results:
Sub lethal effects of exposure were observed at both the control and 100mg/L test concentrations. These responses inclduing sitting and swimming at the bottom of the tanks.
Validity criteria fulfilled:
The acute toxicity of the test item to the freshwater fish rainbow trout (Oncorhynchus mykiss) has been investigated and nominal test concentrations gave a 96-Hour LC50 of greater than 100 mg/L. The No Observed Effect Concentration was 100 mg/L.
Executive summary:


This study was performed to assess the acute toxicity of the test item to rainbow trout. The method was designed to be compatible with the OECD guideline for testing of chemicals (1992) No 203 "Fish,acute toxicity test" referenced as Method C.1 of commission regulation (EC) no 440/2008.

Results and conclusion

Exposure of the rainbow trout to the test item gave LC50 values based on the nominal test concentrations of > than 100mg/L. The No Observed Effect Concentration was 100mg/L.

Description of key information

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
100 mg/L

Additional information