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EC number: 204-024-4 | CAS number: 113-24-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2018-04-10 to 2018-05-10
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Version / remarks:
- 1996
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Four replicate samples (3 mL per replicate) were taken from the test solution (100 mg/L) and from the control at the start and at the end of the test.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
The test solution used in the test was prepared by mechanical dispersion without using of any solubilising agent. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Waterflea
- Strain/clone: Straus
- Source: Laboratory of Hydrobiology (Central Agricultural Office, Directorate of Plant-, and Soil Protection) 2100 Gödöllő, Kotlán S. u. 3. Hungary
- Age at test start: Less than 24 hours old
- Feeding during test: no
ACCLIMATION
- Acclimation period: no, breeding conditions same as test conditions - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No
- Hardness:
- 249 mg/L (as CaCO3)
- Test temperature:
- 20.1 - 20.5 °C
- pH:
- 8.02 – 8.30
- Dissolved oxygen:
- 7.09 – 7.27 mg/L
- Salinity:
- Not applicable
- Conductivity:
- Not measured
- Nominal and measured concentrations:
- Nominal concentration: 100 mg/L
Measured concentration: 97.8 mg/L at the start and 102.9 mg/L at the end of the test (98 and 103 % of the nominal respectively) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers
- Material, fill volume: glass, 40 mL medium
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: ISO medium, according to OECD 202
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light (artificial illumination) and 8 hours darkness
- Light intensity: 651 lux
EFFECT PARAMETERS MEASURED
- Visual observation of immobility or mortality after 24 and 48 h of exposure
RANGE-FINDING STUDY
- Test concentrations: 100 mg/L nominal concentration
- Results used to determine the conditions for the definitive study: No immobility or any sub-lethal effect was detected in the test concentration and in the control group during the 48-h exposure period. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: no
- Observations on body length and weight: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no
VALIDITY OF THE STUDY
- The immobilisation rate of the introduced Daphnia did not exceed 10 %. Immobility was not observed in the untreated control and no Daphnia were trapped at the water surface or showed any sign of stress.
- The dissolved oxygen concentration at the end of the test was more than 3 mg/L in control and test vessels.
All validity criteria were within acceptable limits and therefore the study was considered as valid. - Results with reference substance (positive control):
- Date of the last study with reference item Potassium dichromate was: 28 February – 01 March 2018. The 24h EC50: 1.41 mg/L, (95 % confidence limits: 1.19 – 1.68 mg/L).
- Reported statistics and error estimates:
- A limit test was performed and toxic effects were not observed, therefore statistical analysis was not performed. The NOEC, LOEC and ECx values of the test item were determined directly from the raw data.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute immobilisation of the test item to Daphnia magna was determined according to OECD guideline 202, EU Method C.2 and EPA OPPTS 850.10110 in compliance with GLP principles. The EC50 value after 48 h was determined to be > 100 mg/L (nominal).
- Executive summary:
The acute immobilisation of the test item to Daphnia magna (STRAUS) was determined in compliance with GLP criteria according to OECD Guideline 202, EU Method C.2 and EPA OPPTS 850.1010. The study was conducted under static conditions over a duration of 48 hours. 20 test organisms were exposed to the test concentration and control. The test item solutions were clearly dissolved throughout exposure. The nominal test item concentration was: 100 mg/L. A reference test was carried out with potassium dichromate as reference item at least twice a year and the reference toxicity determined. The EC50 -value of the reference item at 1.41 mg/L after 24 h was within the prescribed concentration range of 1.0 to 2.5 mg/L according to AQS, DIN Guideline 38412 L 30. Water quality parameters of pH-value and dissolved oxygen concentration measured at 0 and 48 h were determined to be within the acceptable limits.The validity criteria of the test guideline were fulfilled. The EC50 value and EC10 value were determined to be above 100 mg/L after 48 h.
Reference
Table 1: Immobilisation of test animals
Test group | Replicate | Number of treated animals | Number of immobilised animals | |
24 h | 48 h | |||
Control | 1 | 5 | 0 | 0 |
2 | 5 | 0 | 0 | |
3 | 5 | 0 | 0 | |
4 | 5 | 0 | 0 | |
100 mg/L (nominal) | 1 | 5 | 0 | 0 |
2 | 5 | 0 | 0 | |
3 | 5 | 0 | 0 | |
4 | 5 | 0 | 0 |
Table 2: Test item concentration measured
Sampling date |
Nominal |
Mean of the measured concentrations |
Measured concentration in percentage of the nominal |
Relative Standard Deviation [%] (n=4) |
10 April 2018 |
100 |
97.8 |
98 |
1 |
12 April 2018 |
100 |
102.9 |
103 |
4 |
The test item was not detected in the control samples.
Description of key information
The 48-h EC50 of the substance was determined to be > 100 mg/L (nominal).
Key value for chemical safety assessment
Additional information
The acute immobilisation of the test item to Daphnia magna (STRAUS) was determined in compliance with GLP criteria according to OECD Guideline 202, EU Method C.2 and EPA OPPTS 850.1010. The study was conducted under static conditions over a duration of 48 hours. 20 test organisms were exposed to the test concentration and control. The test item solutions were clearly dissolved throughout exposure. The nominal test item concentration was: 100 mg/L. A reference test was carried out with potassium dichromate as reference item at least twice a year and the reference toxicity determined. The EC50 -value of the reference item at 1.41 mg/L after 24 h was within the prescribed concentration range of 1.0 to 2.5 mg/L according to AQS, DIN Guideline 38412 L 30. Water quality parameters of pH-value and dissolved oxygen concentration measured at 0 and 48 h were determined to be within the acceptable limits. The validity criteria of the test guideline were fulfilled. The EC50 value and EC10 value were determined to be above 100 mg/L after 48 h (reference 6.1.3-1).
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