2,4-difluoroaniline

Toxicity to aquatic plants other than algae

Administrative data

Endpoint:

toxicity to aquatic plants other than algae

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 221 (Lemna sp. Growth Inhibition Test)

Version / remarks:

23 Mar 2006

Deviations:

yes

Remarks:

see "Principles of method if other than guideline"

Principles of method if other than guideline:

Due to a technical failure of a component of the automatically climate acquisition system, no temperature data were recorded in the climate chambers from 2015-06-19 (ca. 10 a.m.) to 2015-06-23 (ca 5 p.m.). The study was placed in the climate chamber from 2015-06-19 to 2015-06-26. Since the technical failure was not known at that time, no temperature data for the respective time period were recorded. The recorded temperature data after re-activation of the automatically climate acquisition system at 2015-06-19 (ca 5 a.m.) were well in-line of the recommended range. Additionally, the historical temperature data for the climate chambers showed a stable running system and, the control performance of the respective test is well in-line with the performance recommended in the guidelines. Therefore it is highly probable that the temperature given in the climate chambers were within the recommended temperature range and it can be concluded that this deviation did not invalid the whole test.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2,4-difluoroaniline

EC Number:

206-687-5

EC Name:

2,4-difluoroaniline

Cas Number:

367-25-9

Molecular formula:

C6H5F2N

IUPAC Name:

2,4-difluoroaniline

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Lemna gibba

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

7 d

Test conditions

Nominal and measured concentrations:

Nominal: 0.954, 3.05, 9.77, 31.3, and 100 mg p.m./L
Measured: 90 - 106% (freshly) and 100 - 103% (aged) of nominal

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

7 d

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other:

Remarks:

effect on mean growth rate of frond number and mean growth rate of total frond area of plants

Effect concentrations 2

Duration:

7 d

Dose descriptor:

NOEC

Effect conc.:

31.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other:

Remarks:

effect on mean growth rate of frond number

Effect concentrations 3

Duration:

7 d

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other:

Remarks:

effect on mean growth rate of total frond area of plants

Effect concentrations 4

Duration:

7 d

Dose descriptor:

EC10

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other:

Remarks:

effect on mean growth rate of frond number and mean growth rate of total frond area of plants

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

No sublethal effects on Lemna gibba were observed. Only minor effects on frond number occurred (inhibition < 10%) within the test duration. The resulting ErC50 values for frond number and frond area are higher than the highest test concentration of 100 mg p.m./L. The lowest NOErC value is 31.3 mg p.m./L for total frond number and with a corresponding LOErC of 100 p.m./L.