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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 222 (Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei))
Version / remarks:
13 Apr 2004
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2,4-difluoroaniline
EC Number:
206-687-5
EC Name:
2,4-difluoroaniline
Cas Number:
367-25-9
Molecular formula:
C6H5F2N
IUPAC Name:
2,4-difluoroaniline

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
yes
Remarks:
acetone

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
56 d

Test conditions

Nominal and measured concentrations:
Solvent control, water control, 7.8, 15.6, 31.3, 62.5. 125, 250, 500 and 1000 mg pure metabolite /kg soil
Solvent control, water control, 8.0, 16.0, 32.0, 63.9, 127.8, 255.6, 511.2, and 1022.5 mg test item/kg soil

Results and discussion

Effect concentrationsopen allclose all
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
500 other: mg pure metabolite/kg soil dry weight
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
>= 500 other: mg pure metabolite/kg soil dry weight
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: weight and feeding activity
Duration:
56 d
Dose descriptor:
NOEC
Effect conc.:
125 other: mg pure metabolite/kg soil dry weight
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
No mortality was observed in any treatment group except of the concentration of 1000 mg pure metabolite/kg (28% mortality).
No effects on body weight could be observed after 4 weeks exposure.
No statistically significant effects on reproduction were observed up to the concentration of 125 mg pure metabolite/kg soil.
The feeding activity in all the treated groups was comparable to the control.
The NOEC (28 d) for weight changes and feeding activity of the earthworm Eisenia fetida was ≥ 500 mg pure metabolite/kg soil, i.e. the highest concentration tested.
The NOEC (28 d) for mortality was 500 mg pure metabolite/kg soil. The NOEC (56 d) for reproduction was 125 mg pure metabolite/kg.