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Long-term toxicity to aquatic invertebrates

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Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2005
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Remarks:
EPA/600/4-90/027 and ASTM's Methods E729, 1997, Volume 11.05
Qualifier:
according to guideline
Guideline:
other: EPA/600/4-90/027 and ASTM's Methods E729, 1997, Volume 11.05
Deviations:
not specified
Principles of method if other than guideline:
see EPA/600/4-90/027, Methods for Measuring the Acute Toxicitv of Effluents and Receiving Waters to Freshwater Organisms, and by ASTM's Methods E729, 1997, Volume 11.05, Annual Book of ASTM Standards. Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians.
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
not specified
Details on test solutions:
The stock solutions used for the test solutions were prepared by diluting a known weight (grams) of the test material to a known volume. methanol as the solvent carrier was used.
Fourth Edition, by GLEC's (Great Lakes Environmental Center) Standard Operating Procedure for Stock Solution Preparation for Polymers (Detergents and Chemical Additives).
Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
TEST ORGANISM
- Common name: Ceriodaphnia
- Strain/clone: Ceriodaphnia dubia
- Source: no data available
- Age at study initiation (mean and range, SD): less than 24 hours old
- Feeding during test
- Food type: no data available
- Amount: no data available
- Frequency: no data available

ACCLIMATION
- Acclimation period: no data available
- Acclimation conditions (same as test or not): no data available
- Type and amount of food during acclimation: no data available
- Feeding frequency during acclimation: no data available
- Health during acclimation (any mortality observed): no data available

QUARANTINE (wild caught)
- Duration: no data available
- Health/mortality: no data available








Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
7 d
Hardness:
no data available
Test temperature:
25 +/- 1°C
pH:
no data available
Dissolved oxygen:
no data available
Salinity:
no data available
Conductivity:
no data available
Nominal and measured concentrations:
Nominal concentrations: 0.34; 0.68; 1.36; 2.73 and 5.45 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable):closed
- Material, size, headspace, fill volume: covered glass 30 mL beakers or plastic cups containing 15 mL of the test solution or control
- Aeration: no, The test solutions were not aerated, although DO concentration was determined prior to the experiment and adjusted to above 40% saturation.
- Renewal rate of test solution (frequency/flow rate): 24 hour-renewal. . The test solutions were renewed daily during each test, accomplished by placing the newly prepared test solutions in clean beakers with food and transferring the adult C. dubia into the new test solutions.
- No. of organisms per vessel: 1; 10 beakers, one organism per beaker containing 15 mL test solution, were used for each test concentration and for the control.
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): no data available
- Biomass loading rate: no data available

TEST MEDIUM / WATER PARAMETERS
No data available

OTHER TEST CONDITIONS
- Adjustment of pH: Sodium hydroxide and hydrochloric acid were used to adjust the pH of the test solution, prior to testing, if needed.
- Photoperiod: A 16-hour light: 8 hour dark photoperiod was automatically controlled to regulate light and photoperiod.
- Light intensity: no data available

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Reproduction was monitored by counting the number of live and dead young, which were discarded each time the adults were transferred to a new test solution.
NOEC, LOEC and LC50 values were calculated based on adult survival, reproduction

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 0.5
- Range finding study
- Test concentrations: A range-finding toxicity test was conducted initially on each fragrance chemical at one to three times the estimated water solubility to determine the test concentrations for the definitive chronic toxicity tests.
- Results used to determine the conditions for the definitive study: Based on the results of the range-finding tests, we initiated the definitive chronic toxicity tests with C. dubia using three times the estimated water solubility as the highest test concentration and a dilution factor of 0.5 to produce test concentrations.
Reference substance (positive control):
not specified
Key result
Duration:
7 d
Dose descriptor:
LC50
Effect conc.:
1.93 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
7 d
Dose descriptor:
NOEC
Effect conc.:
0.68 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Key result
Duration:
7 d
Dose descriptor:
LOEC
Effect conc.:
1.36 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Details on results:
no other data
Results with reference substance (positive control):
no data available
Reported statistics and error estimates:
no data available
Validity criteria fulfilled:
yes
Remarks:
Survival in the laboratory control must be 80 percent and reproduction in controls must average 15 or more young per surviving female, 60 percent of surviving females in the controls must produce 3 broods
Conclusions:
With a NOEC-7days: 0.68 mg/L, isobornylcyclohexanol is toxic to aquatic invertebrates with long lasting effects.
Executive summary:

A static renewal Ceriodaphnia dubia chronic toxicity test (7-days) was performed with Iso bornyl cyclo hexanol according to EPA/600/4-90/027 and ASTM's Methods E729, 1997, Volume 11.05.

Results:

- LC50: 1.93 mg/L

-       NOEC: 0.68 mg/L (based on reproduction)

-       LOEC: 1.36 mg/L (based on reproduction)

With a NOEC-7days: 0.68 mg/L, isobornylcyclohexanol is toxic to aquatic invertebrates with long lasting effects.

Description of key information

The test item, isobornylcyclohexanol, was considered toxic to aquatic invertebrates with long lasting effects.

The 7-d NOEC of isobornylcyclohexanol to Ceriodaphnia dubia was determined to be 0.68 mg/L based on the reproduction; revealing it is toxic to aquatic invertebrates with long lasting effects.

Key value for chemical safety assessment

Additional information

A study, scored as Klimisch 2 and flagged as a key study, is available on isobornylcyclohexanol, giving a 7-d-NOEC : 0.68 mg/L and revealing that isobornylcyclohexanol is toxic to aquatic invertebrates with long lasting effects (S.C. Johnson, 2005).

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