Registration Dossier

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
See read-across record in section 13.
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Remarks on result:
other:
Remarks:
Result from read-across source substance Cas No. 30798-32-4
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
609.88 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% confidence interval 565.2 mg/L - 658.3 mg/L
Remarks:
Result from read-across source substance CAS No. 102-71-6

Description of key information

Results for the short-term toxicity to aquatic invertebrates of the substance (CAS no. 29340-81-6), were based on results of the dissociation product CAS no. 29340-81-6, namely triethanolamine (TEA, CAS no. 102-71-6) and structural analogue 2-hydroxypropyl)ammonium citrate (MIPA citrate; CAS no. 30798-32-4). For these substances, short-term toxicity guideline studies are available for aquatic invertebrates. The 48-h EC50 value for TEA in Daphnia magna is 609.88 mg/L (Warne et al., 1999), the 48-h EC50 value for MIPA citrate in Daphnia magna is > 100 mg/L (Charles River Laboratories, 2017). The lowest value of 100 mg/L will be used in the assessment.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
100 mg/L

Additional information

No substance-specific data on theshort-term toxicity to aquatic invertebratesof the substance (CAS 29340-81-6) are available. However, according to Article 13 of legislation EC1907/2006, in case no appropriate animal studies are available for assessment, information should be generated whenever possible by means other than vertebrate animal tests, i. e. applying alternative methods such as in vitro tests, QSARs, grouping and read-across. CAS 29340-81-6 is an organic compound manufactured from triethanolamine (TEA, CAS 102-71-6) and citric acid (CAS 77-92-9) and dissociates into the respective TEA cation and citrate anion. Therefore it is considered to be acceptable to derive lacking information on toxicological properties of CAS 29340-81-6 by read-across from TEA and close analogue2-hydroxypropyl)ammonium citrate (MIPA citrate; CAS no. 30798-32-4), which dissociates into citric acid in water.

For TEA, the short-term toxicity to aquatic invertebrates was determined in a study according to ASTM Designation E1192 (GLP not specified)(Warne et al., 1999). In this study, groups of 15 daphnids per concentration (Ceriodaphnia dubia) were exposed for 48 hours to the test substance (concentration unknown) under static conditions. The number of immobile daphnids were counted at the end of test. The EC50 value after 48 hours in this study was determined to be 609.88 mg/L (95% CI: 565.5 - 658.3 mg/L).

For MIPA citrate, the acute toxicity to aquatic invertebrates was determined in a study according to OECD TG 202 and in compliance with GLP criteria (Ibacon, 2017). In this study daphnids (D. magna, 20 per concentration) were exposed to nominal concentrations of 0 (control) and 149 mg test item/L for 48 hours under static condition. The test material as such exists as aqueous solution: the test material concentration corresponds to a nominal concentration of 100 mg/L based on active ingredient (a.i.). Analytical confirmation of nominal test concentrations showed that all test concentrations remained well within ± 20% of nominal concentrations throughout the test. Therefore, effect concentrations are expressed as nominal. Immobilization was recorded after 24 and 48 hours exposure. No immobilisation of the test animals was observed in the control nor in the only test concentration of 100 mg a.i./L. Based on these findings the 48-h EC50 value was determined at > 100 mg a.i./L.

As a worst-case approach, the 48-h EC50 value of 100 mg/L is used in the derivation of PNECs of TEA citrate.