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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Batch No.: 0900243308
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
Aerobic activated sludge (microorganisms from a domestic wastewater treatment plant) was supplied by the sewage treatment plant of Rossdorf, Germany.
Duration of test (contact time):
28 d
Initial conc.:
104.2 mg/L
Based on:
test mat.
Initial conc.:
180.2 mg/L
Based on:
ThOD
Remarks:
ThODNH4
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
The test item Resorcinol diacetate was investigated for its ready biodegradability in a manometric respirometry test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
Reference substance:
benzoic acid, sodium salt
Parameter:
% degradation (O2 consumption)
Remarks:
ThODNH4 (theoretical oxygen demand)
Value:
10
Sampling time:
1 d
Remarks on result:
other: readily biodegradable
Key result
Parameter:
% degradation (O2 consumption)
Remarks:
ThODNH4 (theoretical oxygen demand)
Value:
72
Sampling time:
10 d
Remarks on result:
other: readily biodegradable
Parameter:
% degradation (O2 consumption)
Remarks:
ThODNH4 (theoretical oxygen demand)
Value:
81
Sampling time:
28 d
Remarks on result:
other: readily biodegradable
Details on results:
The mean biodegradation of 10% of Resorcinol diacetate was reached at day 1 (ThODNH4). At the end of the 10-day window at day 11, the degradation of Resorcinol diacetate was 72% (ThODNH4), the 10 day window criterion was therefore passed. The mean biodegradation at test end after 28 days was 81% (ThODNH4).
Parameter:
BOD5
Value:
25 other: mg O2/L
Remarks on result:
other: Cumulative Biochemical Oxygen Demand after 1 day
Key result
Parameter:
BOD5
Value:
140 other: mg O2/L
Remarks on result:
other: Cumulative Biochemical Oxygen Demand after 10 days
Parameter:
BOD5
Value:
160 other: mg O2/L
Remarks on result:
other: Cumulative Biochemical Oxygen Demand after 28 days
Parameter:
BOD5*100/ThOD
Value:
14 other: %
Remarks on result:
other: Percentage Biodegradation based on ThODNH4 after 1 day
Key result
Parameter:
BOD5*100/ThOD
Value:
74 other: %
Remarks on result:
other: Percentage Biodegradation based on ThODNH4 after 10 days
Parameter:
BOD5*100/ThOD
Value:
79 other: %
Remarks on result:
other: Percentage Biodegradation based on ThODNH4 after 28 days
Results with reference substance:
Biodegradation based on ThODNH4 of the reference item sodium benzoate: 29% after 1 day; 84% after 10 days and 90% after 28 days.
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
The degradation rate of Resorcinol diacetate did reach 60% within the 10-day window. Therefore, Resorcinol diacetate is considered to be readily biodegradable.
Executive summary:

Resorcinol diacetate is considered to be readily biodegradable.

Description of key information

The mean biodegradation of 10% of Resorcinol diacetate was reached at day 1 (ThODNH4). At the end of the 10-day window at day 11, the degradation of Resorcinol diacetate was 72% (ThODNH4), the 10 day window criterion was therefore passed. The mean biodegradation at test end after 28 days was 81% (ThODNH4).

Therefore, Resorcinol diacetate is considered to be readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information