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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
2008
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
adopted April 13, 2004
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Batch number: 0900250090
Date of receipt: 2017-02-06
Expiry date: 2018-01-20
Water solubility: 7.398 g/L at 20 °C
Stability of test concentration/s during exposure: Examined by chemical analysis (HPLC) in the freshly prepared media on 2017-12-05 and 2017-12-06 and in the media after 24 hours of exposure on 2017-12-06 and 2017-12-07 according to the semi-static test conditions.
Analytical monitoring:
yes
Details on sampling:
Stability of test concentration/s during exposure: Examined by chemical analysis (HPLC) in the freshly prepared media on 2017-12-05 and 2017-12-06 and in the media after 24 hours of exposure on 2017-12-06 and 2017-12-07 according to the semi-static test conditions.
Vehicle:
no
Details on test solutions:
Pre-treatment of test item and preparation of test item concentrations
A stock solution was prepared to give the desired series of test concentrations. 10.4 mg of the test item were added to 1 litre of dilution water on 2017-12-04 and 10.6 mg of the test item were added to 1 litre of dilution water on 2017-12-05, treated for 60 seconds at 8000 rpm with an ultra turrax and stirred for 24 h on a magnetic stirrer. Undissolved particles of the test item were removed by filtration using folded filters with a pore size of 7 - 12 µm. The pH was measured to be 7.8 on 2017-12-05 and 2017-12-06.
To produce the different test item concentrations appropriate amounts of the stock solution were diluted with dilution water to a volume of 500 mL. 50 mL of the prepared solutions were taken per replicate and 0.5 mL of dilution water containing 5 daphnids was given to all replicates resulting in the final nominal concentrations. For each test item concentration and the control 4 replicates were prepared.
Test organisms (species):
Daphnia magna
Details on test organisms:
Name: Daphnia magna STRAUS, parthenogenetic females
Source: Strain of Bundesgesundheitsamt Berlin
Maintenance and Acclimatisation: A population of parthenogenetic females of synchronized age structure has been maintained for more than 20 years in the test facility under constant temperature conditions (20 +/- 2 °C) at a 16 : 8 hour light-dark photoperiod (light intensity: < 20 µE x m-2 x s-1). The culture water (so-called 'M4 medium') was partly renewed once a week. The Daphnia were exclusively fed unicellular green algae (Desmodesmus subspicatus) 'ad libitum'. Mortalities of parent Daphnia during the culture period were recorded daily. The neonates were separated from their parent Daphnia by filtration prior to the acute test.
Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
48 h
Hardness:
14.5 °dH (= 259 mg/L CaCO3)
Test temperature:
18 - 22 °C +/- 1 °C
pH:
7.7 - 8.1
Dissolved oxygen:
8.5 mg/L - 8.8 mg/L (100 - 97%)
Nominal and measured concentrations:
0.016, 0.031, 0.063, 0.125, 0.25, 0.5, 1 and 2 mg/L (nominal)
Details on test conditions:
The main test was conducted as a semi-static test with daily renewal of test medium.
During the test a temperature range of 18 - 22 °C was maintained in the test vessels, with a maximum temperature fluctuation of +/- 1 °C in each individual test.
The temperature, the pH and the oxygen values were measured every day in the freshly prepared media and in the media after 24 hours of exposure.

Exposure conditions
Test vessels : 100 mL glass beakers covered with watch glasses holding 5 neonates in 50 mL of test medium
Experimental design: 8 test concentrations plus 1 control
5 neonates per vessel, 4 replicates per concentration/control
no feeding during the exposure period
semi-static system
Method of initiation: neonates were placed in prepared media
Photoperiod: 16 h light : 8 h dark
Temperature of incubation unit: 19.6 to 20.0 °C
Aeration: none
Test item concentration/s: 0.016, 0.031, 0.063, 0.125, 0.25, 0.5, 1 and 2 mg/L (nominal)
Method of administration: stock solution (10 mg/L)
Medium renewal: daily
Duration of exposure: 48 hours
Criteria of effects: The criterion of adverse effects used in this study was the item-induced alteration of the normal mobility behaviour and the loss of locomotory actions of the neonates, observed at 24 and 48 hours.
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
339.4 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% confidence limits: 272.9 - 420.4 ug/L
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
1 121.9 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
141.4 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
1 172.2 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% confidence limits: 966.3 - 1441.4 [µg/L]
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
> 2 023.2 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
562.8 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Defined concentrations of the test item led to a certain percentage proportion of immobilised Daphnia at the end of the 48 hour study period. Immobilisation rates were recorded at 24-hour intervals. Additionally any abnormal behaviour or appearance of the Daphnia was reported.
The results are expressed in terms of geometric mean measured concentrations calculated on base of the sum of Resorcindiacetate, Resorcinmonoacetate and Resorcin.
Effective concentrations calculated as sum of Resorcindiacetate, Resorcinmonoacetate and Resorcin ranged from 108.6 % to 175.3 % in the freshly prepared media and from 38.7 % to 304.8 % in the media after 24 hours of exposure.
Reported statistics and error estimates:
The EC 50 was calculated by probit analysis using the statistics programme ToxRatPro Version 2.10 (released 2010-09-10).

Results of the probit analysis

A) 24h

Results of the probit analysis: Selected effective concentrations (ECx) of the test item and their 95%-confidence limits (according to Fieller`s theorem).

 Parameter  EC10  EC20  EC50
 Value [µg/L]  638.251  786.354  1172.177
 lower 95%-cl  414.494  568.549  966.304
 upper 95%-cl  800.913  955.273  1441.391

B) 48h

Results of the probit analysis: Selected effective concentrations (ECx) of the test item and their 95%-confidence limits (according to Fieller`s theorem).

 Parameter  EC10  EC20  EC50
 Value [µg/L]  164.760  211.156 339.423
 lower 95%-cl  107.103  150.661 227.896
 upper 95%-cl  213.117 263.717  420.389
Validity criteria fulfilled:
yes
Remarks:
The immobilisation and other abnormalities in the controls did not exceed 10 % by the end of the test. The dissolved oxygen concentration remained above 3 mg/L throughout the exposure period.
Conclusions:
A study was performed to assess the acute toxicity of Resorcindiacetat to Daphnia magna STRAUS under semi-static conditions and showed an EC50 value of 215.8 µg/L after 48 hours.
Executive summary:

A study was performed to assess the acute toxicity of Resorcindiacetat to Daphnia magna STRAUS under semi-static conditions.

The study was conducted in accordance with the Council Regulation (EC) No 440/2008, Method C.2 ‘Acute toxicity for Daphnia’ (2008) which is equivalent to OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test' (adopted April 13, 2004).

The Daphnia were exposed to a range of concentrations, nominally 0.016, 0.031, 0.063, 0.125, 0.25, 0.5, 1 and 2 mg/L of Resorcindiacetat dissolved in dilution water. Auxiliaries used to prepare the test media were an ultra turrax, a magnetic stirrer and a folded filter.

Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure. The following values were determined:

 Time [h]  EC50 [µg/L]
 24  1172.2
 48 339.423

The results are expressed in terms of geometric mean measured concentrations calculated on base of the sum of Resorcindiacetate, Resorcinmonoacetate and Resorcin.

Effective concentrations calculated as sum of Resorcindiacetate, Resorcinmonoacetate and Resorcin ranged from 108.6 % to 174.6 % in the freshly prepared media and from 38.7 % to 255.5 % in the media after 24 hours of exposure.

The hardness of the dilution water used was 14.5 °dH (= 259 mg/L CaCO3).

Description of key information

A study was performed to assess the acute toxicity of Resorcindiacetat to Daphnia magna STRAUS under semi-static conditions.

The study was conducted in accordance with the Council Regulation (EC) No 440/2008, Method C.2 ‘Acute toxicity for Daphnia’ (2008) which is equivalent to OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test' (adopted April 13, 2004).

The Daphnia were exposed to a range of concentrations, nominally 0.016, 0.031, 0.063, 0.125, 0.25, 0.5, 1 and 2 mg/L of Resorcindiacetat dissolved in dilution water. Auxiliaries used to prepare the test media were an ultra turrax, a magnetic stirrer and a folded filter.

Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure. The following values were determined:

 Time [h]  EC50 [µg/L]
 24  1172.2
 48  215.8

The results are expressed in terms of geometric mean measured concentrations calculated on base of the sum of Resorcindiacetate, Resorcinmonoacetate and Resorcin.

Effective concentrations calculated as sum of Resorcindiacetate, Resorcinmonoacetate and Resorcin ranged from 108.6 % to 175.3 % in the freshly prepared media and from 38.7 % to 304.8 % in the media after 24 hours of exposure.

The hardness of the dilution water used was 14.5 °dH (= 259 mg/L CaCO3).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
339.4 µg/L

Additional information