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EC number: 947-655-7 | CAS number: -
At the Fraunhofer Institute for Molecular Biology and Applied Ecology the biodegradation of PC-2015 -597 was investigated during a 28-day period in a Manometric Respirometry Test according to EC method C.4-D (440/2008/EEC) and OECD guideline 301 F (1992). Standard OECD 301 test medium was inoculated with 29.6 mg dry mass/L non-adapted microorganisms from a digester of a sewage treatment plant mainly fed with municipal wastewater (STP: Ruhrverband Kläranlage, Sunthelle 6, 57392 Schmallenberg, Germany). The test solutions were stirred in closed flasks at 22 °C ± 1 °C for 28 days. The rate of degradation was monitored by measuring the quantity of oxygen required to maintain a constant gas volume in the respirometer flasks over a 28-d period. For that purpose a SAPROMAT respirometer (VOITH Inc.) was used. The amount of oxygen taken up by the microbial population during biodegradation of the test item was expressed as a percentage of the ThOD (Theoretical Oxygen Demand). The following test concentrations were used: (1) 272 mg/L (ThOD = 140 mg/L, in duplicate) and (2) 112 mg/L (ThOD = 58 mg/L, in duplicate), respectively, corrected for uptake by the blank inoculum run in duplicate. The second concentration was chosen for the following reason: Some substances with high ThOD’s have gently inclined degradation curves, even when they are completely degradable. However, for these substances it is hard to reach the threshold value within the 10 -day window. Therefore, an additional assay with 112 mg test item (58 mg ThOD) was added. This concentration meets the required concentration of 50 - 100 mg ThOD/L acc. to OECD 301 F. In order to check the procedure, sodium benzoate (in duplicate) was used as a degradable reference item at a concentration of 100 mg/L. Furthermore, an abiotic control in duplicate was used. The pH values was 7.4 °C ± 2 throughout the study.
The oxygen uptake of the inoculum blank was average 22 mg/L in 28 days and the pH value was inside the range of 6.8 - 8.1. The degradation of the reference substance sodium benzoate had reached average 88 % (SD= 2.1) within the first 14 days, i.e. the pass level of 60%. With 2 and 17 %, the difference of extremes of replicate values for the removal of test item was less than 20 % for both concentrations, respectively. Hence, the test is valid according to the validity criteria of the guideline. The biodegradation of 272 mg test item per litre (140 mg/L ThOD, concentration ) in the static test was found to be at average 80.5 % (71.9 and 89.1% in replicate vessels). A plateau was not yet reached on day 28. On day 28, concentration (2) (112 mg/L test substance, 58 mg/L ThOD) showed higher biodegradation values, average 93.8% (92.9 and 94,7% in replicate vessels). A plateau was not yet reached after 28 days. The 10% generic trigger value for biodegradation initiation was reached within latest 2 days after test initiation. Within the day 10 -window, average 72% and 77% biodegradation occurred, respectively. No inhibitory effects of the test item were observed in the toxicity control where more than 25 % degradation occurred within 14 days. In conclusion, the substance is readily biodegradable..
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