Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
For justification please refer to Read Across Justification in IUCLID section 13.
Reason / purpose for cross-reference:
read-across source
Key result
48 h
Dose descriptor:
Effect conc.:
> 100 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:

Description of key information

The acute toxicity of the read across substance Reaction products of γ-butyrolactone, naphthalene-1,6-diol and phthalic anhydride (EC 946-798-2) to aquatic invertebrates was determined according OECD 202 under GLP conditions. The 48-h EL 50 was determined to be > 100.0 mg/L.

Key value for chemical safety assessment

Additional information

No data on toxicity to aquatic invertebrates were available for the substance itself. Therefore, a read across to the close structural analogue Reaction products ofγ-butyrolactone, naphthalene-1,6-diol and phthalic anhydride (EC 946-798-2) was conducted.

Key study

To assess the toxicity of the close structural analogue (EC 946-798-2) to Daphnia magna (STRAUS), an Acute Toxicity Test according to OECD Guideline 202 was carried out in compliance with GLP principles (BASF SE, 2017). The test was conducted under semi-static conditions over a period of 48 hours with the only (limit) concentration of 100 mg/L. In addition, a negative control (without the test item) and a positive control with the reference substance potassium dichromate was performed. Since the test item is a mixture which has a low water solubility, a Water Soluble Fraction (WSF) was prepared. The WSF and the negative control were analytically verified via UPLC-MS/MS in fresh media at the start of the exposure and at renewal (0 and 24 hours) as well as in 24-hours old media at renewal and at the end of the test (24 and 48 hours). The measured concentrations of the test item were 0.0356 and 0.0421 mg test item/L in fresh media (0 and 24 hours) and 0.0187 and 0.0335 mg test item/L in 24-hour old media (24 and 48 hours). Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to the WSF of the test item and the control. Since the close structural analogue contains a large proportion of unidentified by-components, it was treated as a mixture and the results are based on loadings rather than on mean measured concentrations. All effect values are given based on the nominal loading of the test item of 100 mg/L. In result, at the WSF with a nominal loading of 100 mg/L of the test item, no effects on Daphnia magna were observed. Therefore, the 48-h EL50 of the close structural analogue is considered to be > 100 mg/L. The validity criteria of the test guideline were fulfilled.