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EC number: 230-237-7 | CAS number: 6976-37-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No. of test material: Product no. B4429, Lot no. SLBK5235V
- Expiration date of the lot/batch: 30 April 2018
- Purity test date: na
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: no - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg/L
- Sampling method: - no data
- Sample storage conditions before analysis: no information - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: mechanical dispersion
- Eluate: not applicable
- Differential loading: not applicable
- Controls: untreated and reference (tested in September 2016)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): not applicable
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): none
PRELIMINARY TESTS: none - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: 61.81 SAG
- Source (laboratory, culture collection): SAG: Collection of Algal Cultures, 37073 Göttingen, Germany
- Age of inoculum (at test initiation): -
- Method of cultivation: algae were kept as stock culture on solid agar
ACCLIMATION
- Acclimation period: -
- Culturing media and conditions (same as test or not): -
- Any deformed or abnormal cells observed: - none - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- none
- Hardness:
- no data
- Test temperature:
- 22.2 - 22.4°C (measured in the flasks)
22.1 - 23.8°C (in the climate chamber) - pH:
- Start: 7.85 - 7.76 in the five test concentrations(adjusted); 8.18 in the control
End: 8.38 - 8.64 (test concentrations and control) - Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- The measured concentrations showed very good correlation with the nominal concentrations. The recovery rate after 72 hours was 102%.
Therefore the nominal concentration was used for the determination of the results. - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass flasks 250 ml
- Type (delete if not applicable): air-permeable stoppers
- Material, size, headspace, fill volume: -
- Aeration:-
- Type of flow-through (e.g. peristaltic or proportional diluter): none
- Renewal rate of test solution (frequency/flow rate): none
- Initial cells density: 10000 cells/ml
- Control end cells density: approx. 10000000 cells/ml
- No. of organisms per vessel:-
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): -
GROWTH MEDIUM
- Standard medium used: yes
- Detailed composition if non-standard medium was used: -
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted algal growth medium (acc. to OECD TG 201)
OTHER TEST CONDITIONS
- Sterile test conditions: no data
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: 7649 Lux
- Salinity (for marine algae): -
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: manual counting at 24, 48 and 72 h
- Chlorophyll measurement: -
- Other: -
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable
- Justification for using less concentrations than requested by guideline: limit test based on concentration-range finding test
- Range finding study: yes
- Test concentrations: 100 mg/L
- Results used to determine the conditions for the definitive study: no effects up to 100 mg/L in preliminary tests - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): none reported
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- - Results with reference substance valid? YES
- EC50: 0.71 mg/L (ErC50) (tested from September 20 -23, 2016)
- Other: - - Reported statistics and error estimates:
- not applicable as no effects were observed
- Validity criteria fulfilled:
- yes
- Executive summary:
The acute toxicity of the substance was investigated in a GLP-study according to OECD test guideline 201 using
unicellular freshwater green alga Raphidocelis subcapitata.
The study was performed as a static limit test with 100 mg/L with a treatment period of 72 hours. The cell number of each replicate was determined by manual counting. Then the growth rate and the yield were determined.
At the start and at the end of the test, the content of the test item in the test solutions was determined by HPLC. The recovery after 72 hours was 102% of the start concentration. Therefore, the determination of the results was based on the nominal concentrations.
The EC50s of the positive reference control potassium dichromate were tested in a non-current reference test. The values lay within the normal range of the laboratory.
As no toxicity of the substance was observed, the NOEC (growth rate and biomass) of 100 mg/L and the EC50 (growth rate and biomass) of > 100 mg/L were determined.
Reference
The acute toxicity of the substance was investigated in a GLP-study according to OECD test guideline 201 using unicellular freshwater green algae Raphidocelis subcapitata under static conditions.
The study was performed using one concentration containing 100 mg/L with a treatment period of 72 hours. The cell number of each replicate was determined by manual counting. Then the growth rate and the yield were determined.
At the start and at the end of the test, the content of the test item in the test solutions was determined using LC/MS. The recovery after 72 hours was 102 % of the start concentration. Therefore, the determination of the results was based on the nominal concentrations.
The EC50s of the positive reference control potassium dichromate were tested in a non-current reference test. The values lay within the normal range of the laboratory.
As no toxicity of the substancewas observed, a NOEC (growth rate and biomass) of 100 mg/L and an EC50 (growth rate and biomass) of > 100 mg/L were determined.
Detailed results:
Growth rates (µ) and %-inhibition of µ:
growth rates (µ) and %-inhibition of µ | ||||||
Test Group | 0 -24h | 0 -48 h | 0 -72h | |||
µ | % | µ | % | µ | % | |
Control | 0.0345 | 0.0 | 0.0471 | 0.0 | 0.0532 | 0.0 |
100 mg/L | 0.0373 | -8.2 | 0.0465 | 1.3 | 0.0524 | 1.4 |
Yield (y) and %-inhibition of y:
yield (y) and %-inhibition of y | ||||||
Test Group | 0 -72h | |||||
y | % | |||||
Control | 45.3 | 0.0 |
||||
100 mg/L | 42.8 | 5.5 |
Description of key information
In a GLP-study according to OECD test guideline 201 (toxicity to algae, static conditions), the substance did show no toxicity at the test concentration of 100 mg/L. Therefore, it has a NOEC (growth rate and biomass) of 100 mg/L and an EC50 (growth rate and biomass) of >100 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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