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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From May 30 to June 18, 1995
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
1992
GLP compliance:
not specified
Analytical monitoring:
no
Details on test solutions:
Test item was directly dispersed in water.
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: rainbow trout.
- Source: Donnington Fish Farm, Upper Swell, Gloucester U.K.
- Length at study initiation: 4.9 ± 0.2 cm
- Weight at study initiation: 1.49 ± 0.20 g

ACCLIMATION
- Health during acclimation: no deaths during the 7 days prior to the study start.
Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Test temperature:
14 °C
Nominal and measured concentrations:
Nominal 10 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: 20 litres
- Aeration: yes.
- Renewal rate of test solution: daily.
- No. of vessels per concentration: 2 replicates.
- No. of vessels per control : 1 replicate.
- Biomass loading rate: 0.75 g bw/litre

TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: dechlorinated tap water (by passage through an activated carbon filter).
- Hardness: approximately 100 mg/l as CaCO3.

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 1.0, 10 and 100 mg/l
- No. of organisms per concentratyion: 3 fish.
- Results used to determine the conditions for the definitive study: initially a limit test was carried out at 100 mg/l. During the experiment progressive mortalities occurred due to the semi-static conditions employed as opposed to the static test conditions under which the range-finding test was carried out. On repeating the range-finding under semi-static conditions adverse reactions to exposure (i.e. 3/3 fish swimming at the bottom of the test vessel) were observed at the test concentration of 100 mg/l. Therefore, the definitive study was repeated at 10 mg/l to determine a true no effect concentration.
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
10 - 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Sublethal observations / clinical signs:

DEFINITIVE TEST

Concentration (mg/l) Cumulative mortality (initial population: 3)
Control 24 hrs 48 hrs 72 hrs 96 hrs
10 0 0 0 0
10 0 0 0 0

DOSE RANGE FINDING

Concentration (mg/l) Cumulative mortality (initial population: 3)
Control 24 hrs 48 hrs 72 hrs 96 hrs
1 0 0 0 0
10 0 0 0 0
100 0 0 0 0*

*initially a limit test was carried out at 100 mg/l. During the experiment, progressive mortalities occurred due to the semi-static conditions employed as opposed to the static test conditions under which the range-finding test was carried out. On repeating the range-finding under semi-static conditions adverse reactions to exposure (i.e. 3/3 fish swimming at the bottom of the test vessel) were observed at the test concentration of 100 mg/l. Therefore, the definitive study was repeated at 10 mg/l to determine a true no effect concentration.

Conclusions:
LC50 (96h): 10 - 100 mg/l (nominal)
Executive summary:

The acute lethal toxicity of the test substance to rainbow trout was investigated under semi-static exposure conditions over a period of 96 hours, following the OECD guideline 203. Initially a limit test was carried out at 100 mg/l. During the experiment, progressive mortalities occurred due to the semi-static conditions employed as opposed to the static test conditions under which the range-finding test was carried out. On repeating the range-finding under semi-static conditions, testing the concentrations of 1, 10 and 100 mg/l, adverse reactions to exposure (i.e. 3/3 fish swimming at the bottom of the test vessel) were observed at the highest tested concentration. Therefore, the definitive study was repeated at 10 mg/l to determine a true no effect concentration.

No mortality was also observed in the blank control and in the test vessels.

The LC50 was indicated to be between 10 and 100 mg/l.

Conclusion

LC50 (96h): 10 - 100 mg/l (nominal)

Description of key information

Harmful for short-term exposure to fish; LC50 (96h) between 10 - 100 mg/l (nominal)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
10 mg/L

Additional information

The acute lethal toxicity of the test substance to rainbow trout was investigated under semi-static exposure conditions over a period of 96 hours, following the OECD guideline 203. Initially a limit test was carried out at 100 mg/l. During the experiment, progressive mortalities occurred due to the semi-static conditions employed as opposed to the static test conditions under which the range-finding test was carried out. On repeating the range-finding under semi-static conditions, testing the concentrations of 1, 10 and 100 mg/l, adverse reactions to exposure (i.e. 3/3 fish swimming at the bottom of the test vessel) were observed at the highest tested concentration. Therefore, the definitive study was repeated at 10 mg/l to determine a true no effect concentration. No mortality was also observed in the blank control and in the test vessels.

The LC50 was indicated to be between 10 and 100 mg/l.