2,3-dihydroxypropyl methacrylate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The limit concentration (120 mg/L of the test item was weighed out) was freshly prepared with dilution water prior to the start of the exposure (0 hours). The limit concentration was mixed thoroughly by manual agitation.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain/clone: Daphnia magna STRAUS (Clone 5)
- Source: lnstitut tor Wasser-, Boden- und Lufthygiene (WaBolu), 14195 Berlin, Germany
- Breeder: Noack Laboratories GmbH, 31157 Sarstedt, Germany
- Age of parental stock (mean and range, SD): Less than 24 hours old daphnids from a healthy stock were used for the study. Juvenile daphnids were removed from the culture vessels at the latest 24 hours before the start of the exposure and discarded. The juveniles born within the following period of max. 24 hours preceding the exposure were used for the test. No first brood progeny was used for the test.

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers (50 ml)
- Type (delete if not applicable): closed (loosly covered with watch glasses)
- Material, size, headspace, fill volume: 20 ml
- Aeration: no
- No. of organisms per vessel: 4
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16/8 hours light/dark cycle
- Light intensity: diffuse light, light intensity of max. 1500 lx

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other:

Details on results:

The concentrations of the test item Glycerol Monomethacrylate (GMMA) were analytically verified via LC-MS/MS at the start of the exposure (0 hours) and at the end of the exposure (48 hours) in the limit concentration and the control. Details of the analytical method are presented in section 14. The measured concentration of the test item limit concentration was 114 mg/L - corresponding to 95% of the nominal value - at test start and 97.1 mg/L - corresponding to 81 % of the nominal value - at test end. The geometric mean measured concentration was 105 mg/L.

Validity criteria fulfilled:

yes

Conclusions:

Based on the nominal test item concentration of the test item Glycerol Monomethacrylate (GMMA), the 48 hour-EC50 for Daphnia magna was > 120 mg/L. The geometric mean measured concentration was 105 mg/L.

Description of key information

Based on the nominal test item concentration of the test item Glycerol Monomethacrylate (GMMA), the 48 hour-EC50 for Daphnia magna was > 120 mg/L. The geometric mean measured concentration

was 105 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

120 mg/L