Fatty acids tall-oil, reaction products with acrylic acid, potassium salt

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14/5/91 - 16/5/91

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: 40 CFR Part 797.1300, 9/27/85, "Daphnid acute toxicity test" USTC Procedure PRO/ST DAPHNIA 224-7

Deviations:

no

Principles of method if other than guideline:

Not relevant

GLP compliance:

no

Remarks:

Only signatures for GLP test plan available, GLP for test itself unclear

Specific details on test material used for the study:

Not relevant

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 6.25, 12.5, 25, 50 and 100 mg/l (nominal)
- Sampling method: A two milliliter aliquot of each sample solution was withdrawn for analysis

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION:
- Method: No solvent was used
- Controls: Yes

Test organisms (species):

Daphnia pulex

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia pulex
- Source: USTC stock cultures
- Age at study initiation (mean and range, SD): <48 hours
- Method of breeding: Daphnid species are maintained as individual females lines; each female is transferred after releasing each of her first two broods, to new vessels twice, with fresh water and food. Third broods are used, in general, to start the next filial generation and for bioassays.
- No feeding during test

ACCLIMATION
- Acclimation conditions (same as test or not): same
- Type and amount of food: Food consists of a mixture of three algal species: Nitzchia frustulum, Ankistrodesmus convolutus and Chlamydomonas rheinhardi

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

Not relevant

Hardness:

80 - 90 mg/l CaCO3

Test temperature:

22 - 23 °C

pH:

7.6 - 7.8

Dissolved oxygen:

7.8 - 8.8 mg/l

Salinity:

Not relevant

Nominal and measured concentrations:

Nominal: 6.25, 12.5, 25, 50 and 100 mg/l
Measured: 2.4, 4.9, 9.8, 16.3 and 41.3 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: test tube
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glass, 18 x 150 mm containing 15 ml of test medium
- Aeration: Aerated by mixing of test solutions prior to test, no aeration during test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water according to EPA 600/4-85/013 (Moderately-hard reconstituted water):
NaHCO3 - 96 mg/L
CaSO4.2H2O - 60 mg/L
MgSO4 - 60 mg/L
KCl - 4 mg/L

- Alkalinity: 70 - 100 mg/l CaCO3
- Conductivity: 233 - 238 umhos
- Culture medium different from test medium: no
- Intervals of water quality measurement: temperature, oxygen and pH measured at start and end of experiment

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light, 8 hours dark
- Light intensity: fluorescent, 50 - 100 ft candles

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality, morbidity and appearance observed daily

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 41.3 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

act. ingr.

Basis for effect:

mortality

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

22.5 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

act. ingr.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

9.8 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

act. ingr.

Basis for effect:

mortality

Details on results:

- Mortality of control: 0%

Results with reference substance (positive control):

Not relevant

Reported statistics and error estimates:

Probit Analysis or Spearman - Karber Method when possible, otherwise, graphical interpolation.

For detailed results, see attached file "Results.doc".

Validity criteria fulfilled:

yes

Remarks:

Mortality in controls <10%, oxygen saturation >60%, pH differed less than 1 unit during the test

Conclusions:

The acute toxicity LC50 (48h) of Diacid 1550 potassium salt towards Daphnia pulex is 22.5 mg/l based on the active ingredient.

Executive summary:

The acute toxicity of Diacid 1550 potassium salt towards Daphnia pulex was investigated according to 40 CFR Part 797.1300, 9/27/85, "Daphnid acute toxicity test" USTC Procedure PRO/ST DAPHNIA 224-7 under GLP. Daphnids were exposed to nominal concentrations of 6.25, 12.5, 25, 50 and 100 mg/l and observed for 48 hours. Based on measured concentrations, the 24h- and 48h-LC50 and 48h-NOEC were found to be >41.3, 22.5 and 9.8 mg/l, respectively, based on the active ingredient.

Description of key information

The acute toxicity (48h-LC50) of Diacid 1550 potassium salt towards Daphnia pulex is 22.5 mg/l based on the active ingredient.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

22.5 mg/L

Additional information

 The acute toxicity of Diacid 1550 potassium salt towards Daphnia pulex was investigated according to 40 CFR Part 797.1300, 9/27/85, "Daphnid acute toxicity test" USTC Procedure PRO/ST DAPHNIA 224-7 under GLP. Daphnids were exposed to nominal concentrations of 6.25, 12.5, 25, 50 and 100 mg/l and observed for 48 hours. Based on measured concentrations, the 24h- and 48h-LC50 and 48h-NOEC were found to be >41.3, 22.5 and 9.8 mg/L respectively based on the active ingredient