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EC number: 947-663-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From Jan 19th to Feb 10th 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- yes
- Remarks:
- Temperature was outside the range stated in the guideline (17.8 to 21.3 °C instead of 18.0 -22.0 °C). As no immobility in the blank control occurred during the test and all validity criteria were fulfilled, this deviation was stated as uncritical
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Sample Preparation
Sample Preparation concentration 22 mg/L
After adding 6 g of NaCl, 50 mL of a solution in M4 medium was extracted two times with the solvent ethyl acetate (15 and 10 mL), the organic phase was collected into a 20-50 mL pear shaped flask, after drying with Na2SO4 and evaporated to dryness. The residue was then reconstituted using 5 mL of ethyl acetate.
An aliquot (2 mL) of the sample was added to 10 mL volumetric flask, 1 mL of 0.1% phe-nolphthalein in methanol solution; 1 mL of 0.1 M phenyltrimethylammonium hydroxide was added and the flask was filled up to 10 mL with ethyl acetate. Internal standard solution can be added to the test solution before filling the flask. Twofold enrichment was achieved during the sample preparation.
Sample Preparation concentration 10 mg/L
After adding 6 g of NaCl, 100 mL of a solution in M4 medium was extracted two times with the solvent ethyl acetate (15 and 10 mL), the organic phase was collected into a 20 - 50 mL pear shaped flask, after drying with Na2SO4 and evaporated to dryness. The residue was then reconstituted using 5 mL of ethyl acetate.
An aliquot (2 mL) of the sample was added to 10 mL volumetric flask, 1 mL of 0.1% phe-nolphthalein in methanol solution; 1 mL of 0.1 M phenyltrimethylammonium hydroxide was added and the flask was filled up to 10 mL with ethyl acetate. Internal standard solution can be added to the test solution before filling the flask. Fourfold enrichment was achieved during the sample preparation.
Sample Preparation concentration 4.6 mg/L
After adding 6 g of NaCl, 100 mL of a solution in M4 medium was extracted two times with the solvent ethyl acetate (15 and 10 mL), the organic phase was collected into a 20 - 50 mL pear shaped flask, after drying with Na2SO4 and evaporated to dryness. The residue was then reconstituted using 5 mL of ethyl acetate.
An aliquot (4 mL) of the sample was added to 10 mL volumetric flask, 1 mL of 0.1% phe-nolphthalein in methanol solution; 1 mL of 0.1 M phenyltrimethylammonium hydroxide was added and the flask was filled up to 10 mL with ethyl acetate. Internal standard solution can be added to the test solution before filling the flask. Eightfold enrichment was achieved during the sample preparation.
Sample Preparation concentration 2.2 and 1.0 mg/L and the blank control
After adding 6 g of NaCl, 100 mL of a solution in M4 medium was extracted two times with the solvent ethyl acetate (15 and 10 mL), the organic phase was collected into a 20 - 50 mL pear shaped flask, after drying with Na2SO4 and evaporated to dryness. The residue was then reconstituted using 5 mL of ethyl acetate.
The sample was added to 10 mL volumetric flask, 1 mL of 0.1% phenolphthalein in meth-anol solution; 1 mL of 0.1 M phenyltrimethylammonium hydroxide was added and the flask was filled up to 10 mL with ethyl acetate. Internal standard solution can be added to the test solution before filling the flask. Tenfold enrichment was achieved during the sample preparation. - Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species Daphnia magna
Variety STRAUS
Strain Berlin
Arrival of Strain 27. Sep. 2007
Sex female
Age between 0 and 24 hours
The animals which were used in the test did not belong to the first brood of the respective parent animals.
Animal Husbandry
Daphnia magna is bred in the LAUS GmbH throughout the year. The animals were origi-nally obtained from the Umweltbundesamt in Berlin and are kept for the use in toxicity tests. They multiply by parthenogenesis, thus being genetically identical. The keeping is performed similar to the method described in EN ISO 6341, following SOP 115 002 01 (“Zucht und Hälterung von Daphnia magna STRAUS“), edition 12, adopted 02. Feb. 2015.
Vessels glass beakers, nominal volume 2 litres
Medium M4-Medium (recipe of ELENDT)
Food green alga (Desmodesmus subspicatus)
Medium renewal twice a week
Photo period 16/8 hours, using neon tubes
Temperature 20 ±2 °C - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 267 - 356 mg CaCO3/L
- Test temperature:
- 17.8 - 21.3 °C
- pH:
- Experiment 1: 7.4 - 8.5
Experiment 2: 7.3 - 8.1 - Dissolved oxygen:
- Experiment 1: 8.5 - 10.6
Experiment 2: 7.9 - 9.8 - Nominal and measured concentrations:
- Nominal Geometric mean measured
Concentrati Concentration
(mg/L) (mg/L)
1.0 0.33
2.2 0.54
4.6 0.95
10 2.01
22 4.83 - Details on test conditions:
- Duration 21 days
Loading 80 mL/animal, animals were kept individually
Replicates 10 replicates/treatment
Treatments 1.0 / 2.2 / 4.6 / 10 / 22 mg/L nominal concentration
Blank Control M4-medium
Lighting 1000 Lux
Aeration none
Feeding 0.20 mg organic carbon/animal/day, the amount of carbon is based on photometric measurement (linear correlation between absorption at 440 nm and measured DOC con-centration) - Reference substance (positive control):
- no
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 4.83 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- immobilisation
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 4.83 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- immobilisation
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 4.83 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- immobilisation
- Validity criteria fulfilled:
- yes
- Conclusions:
- No toxicity observed at any concentration tested.
NOEC 21d Daphnia > 4.83 mg/L, based on geometric mean of measured highest concentration (nominal: 22 mg/L) - Executive summary:
One valid experiment was performed.
10 Daphnia were exposed to the test item for each treatment and blank control for 21 days in a semi-static test system.
The study was performed using five concentrations ranging from 1.0 to 22 mg/L. The water-accommodated fraction (WAF) was prepared for the test.
The offspring of the animals was counted each working day. Reproduction of the daphnia in the highest treatment was higher than in the blank control.
The content of the test item in the test solutions was determined using GC-MS determination. The analysis of the test item concentrations in the treatments showed a low correlation with the nominal concentrations because of the limited solubility in test medium. Therefore, the determination of the results was based on the geometric mean of the measured concentrations.
According to the OECD guideline, the offspring of dead animals must be taken into account if the death is caused by toxicity of the test item. Only inadvertent and/or accidental parental mortality should be excluded from evaluation. Because no toxicity could be observed, the offspring of deceased parent animals was excluded from the calculation of total offspring per treatment.
The following results were determined for the test item DIACID 1550:
Parameter
Measured Concentration
NOEC
≥4.83 mg/L
LOEC
> 4.83 mg/L
EC50
> 4.83 mg/L
Reference
Findings and Result
Reproduction
In the following table, the summary of the reproduction is given.
Replicate |
Blank Control |
1.0 mg/L |
2.2 mg/L |
4.6 mg/L |
10 mg/L |
22 mg/L |
1 |
75 |
115 |
130 |
78 |
114 |
109 |
2 |
99 |
M |
M |
78 |
112 |
134 |
3 |
98 |
M |
M |
M |
88 |
116 |
4 |
94 |
69 |
91 |
75 |
90 |
104 |
5 |
84 |
89 |
120 |
69 |
129 |
84 |
6 |
117 |
112 |
79 |
74 |
80 |
103 |
7 |
79 |
121 |
93 |
70 |
91 |
123 |
8 |
93 |
130 |
104 |
M |
92 |
101 |
9 |
89 |
105 |
90 |
117 |
119 |
103 |
10 |
120 |
101 |
75 |
94 |
120 |
M |
Mean |
95 |
105 |
98 |
82 |
104 |
109 |
SD |
15 |
19 |
19 |
16 |
17 |
14 |
RSD |
15.53 |
18.30 |
19.61 |
19.73 |
16.45 |
13.25 |
SD = standard deviation
RSD = relative standard deviation = coefficient of variation
M = mortality of parent animal
Summary of Fecundity
In the following table, the total living offspring per treatment and the corresponding living offspring per surviving parent animal and the inhibition in comparison with the control is stated:
Concentration in mg/L |
Total Offspring |
Surviving Parent Animals |
Mean Offspring per Surviving Parent Animal |
Inhibition of Reproduction in % |
Blank Control |
948 |
10 |
95 |
0 |
1.0 |
842 |
8 |
105 |
-11 |
2.2 |
782 |
8 |
98 |
-3 |
4.6 |
655 |
8 |
82 |
14 |
10 |
1035 |
10 |
104 |
-9 |
22 |
977 |
9 |
109 |
-15 |
Negative inhibition values indicate a stimulation of fecundity compared to the control.
Description of key information
NOEC 21 d Daphnia magna (OECD 211): >5.3 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 5.3 mg/L
Additional information
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