Fatty acids tall-oil, reaction products with acrylic acid, potassium salt

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Study period:

12 - 15 May 1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: BIO-D MIC 286-7

GLP compliance:

no

Vehicle:

not specified

Test organisms (species):

activated sludge

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

25 other: % dilution

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

98 g/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth inhibition

Remarks on result:

other: 25% dilution of product (39.18% concentration) is equivalent to 98 g/l

Duration:

48 h

Dose descriptor:

other: Minimum Inhibitory Concentration

Effect conc.:

50 other: % dilution

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

After 48 hours, growth was not inhibited at concentrations up to 25%. No growth was observed in one of the two vessels at 50%, whereas in the other vessel no growth inhibition was observed. Thus the MIC was 50%. No inhition was observed at 25% dilution of the product (NOEC).

Validity criteria fulfilled:

not specified

Conclusions:

The test substance (Diacid 1550 Potassium salt (6339-33), 39.18% concentration) did not inhibit the growth of activated sludge up a 25% dilution of the product. This is recalculated to a concentration for the active of 98 g/L.

Executive summary:

The Minimum Inhibitory Concentration (MIC) was tested according to the method BIO-D MIC 286-7.

After 48 hours contact in water inoculated with sewage microbes, the sample was non-inhibitory to sewage organisms at concentrations of 25% or less.

Description of key information

The test substance (Diacid 1550 Potassium salt (6339-33), 39.18% concentration) did not inhibit the growth of activated sludge up a 25% dilution of the product. This is recalculated to a concentration for the active of 98 g/L.

The Minimum Inhibitory Concentration (MIC) was tested according to the method BIO-D MIC 286-7.

After 48 hours contact in water inoculated with sewage microbes, the sample was non-inhibitory to sewage organisms at concentrations of 25% or less.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

98 g/L