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EC number: 212-379-1 | CAS number: 812-00-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Batch: 34448
Purity: 67.9 - 71.2%
Expiry: 30 March 2022 - Analytical monitoring:
- yes
- Remarks:
- The validated method is fully described in validation report VB18011104G926
- Details on sampling:
- Sample preparation
Before measurement the test solution was diluted tenfold with dilution water, to stay in the
calibrated range.
Miscellaneous
On each sampling day, validity of calibration was controlled by measuring QC samples.
(c = 10 mg/L) Because the mean recovery of the QC samples was lower than ± 3 % of the
nominal value, no sensitivity correction factor from the QC samples was taken into account.
Time 0 & 48h - Vehicle:
- no
- Details on test solutions:
- A test solution containing 100.4 mg/L in dilution water was prepared. Because of the acid
character of the test item, the pH of the test solution was adjusted to 7.8 - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Specification
Species Daphnia magna
Authority STRAUS
Strain Berlin
Sex female
Age between 0 and 24 hours
Origin Umweltbundesamt Berlin
In-house breeding since 27. September 2007
Selection of the test system was made following the proposal of the guidelines.
Animal Husbandry
Daphnia magna is bred in the LAUS GmbH throughout the year. The animals are kept for
the use in toxicity tests. They multiply by parthenogenesis, thus being genetically identical.
The keeping is performed similar to the method described in the OECD guideline.
Vessels preserving glasses, nominal volume 2 L
Medium M4-Medium (recipe of ELENDT)
Food green algae (Desmodesmus subspicatus)
Medium renewal twice a week
Photo period 16/8 hours, using neon tubes
Temperature 20 2 °C
Selection of Daphnia
23 hours and 10 minutes before the start of the test, the adult animals were separated from
the young. 40 minutes before test start, the adults were caught with the help of a glass tube,
and the newborn daphnia (age < 24h) were sieved from the medium and immediately placed
into a beaker containing dilution water. After the settling-in period, animals which showed
no apparent damage were used for the test.
Switching from M4-medium (husbandry) to Dilution water (test) has been shown not to cause
any detrimental effects for test daphnia, therefore no acclimation period of 48 h was performed.
This is a deviation from OECD Guideline No. 202 and EU-Method C.2, which can
be considered uncritical. - Test type:
- static
- Water media type:
- freshwater
- Remarks:
- Elendt M4
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 123.30 MgSO4*7H20
64.80 NaHCO3 - Test temperature:
- 19.0 – 20.5 °C
- pH:
- 7.7 - 7.8
- Dissolved oxygen:
- 8.6 - 8.8 mg/L
- Salinity:
- 293.80 CaCl2*2H20
5.80 KCl - Nominal and measured concentrations:
- Time 0 mean measured concentration was 94% nominal
48h mean measured concentration was 108% nominal - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- No mortality was observed over 48 hours exposure in either the test group or the negative control.
- Results with reference substance (positive control):
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study (202005R201). The 24h-EC50 value was determined as 2.01 mg/L. The
value was within the requested range for the 24h-EC50 between 0.6 - 2.1 mg/L of potassium
dichromate K2Cr2O7 as specified in the OECD guideline. - Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 (48 h) value was determined to be > 100 mg/L
Reference
Description of key information
The LC50 (48 h) value was determined to be > 100 mg/L.
Key value for chemical safety assessment
Additional information
Investigation of short-term toxicity to Daphnia magna is ongoing and results disseminated by ECHA following REACH registration of a similar multiconstituent substance by other legal entities have been used for purposes of risk assessment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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