Registration Dossier

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
no
GLP compliance:
yes
Test type:
fixed dose procedure
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl dihydrogen phosphate
EC Number:
212-379-1
EC Name:
Methyl dihydrogen phosphate
Cas Number:
812-00-0
Molecular formula:
CH5O4P
IUPAC Name:
methyl dihydrogen phosphate
Test material form:
liquid

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
unchanged (no vehicle)
Doses:
Single dose of 2.0 g/kg bw
No. of animals per sex per dose:
Five males and five females
Control animals:
no

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 2 000 mg/kg bw
Based on:
test mat.
Mortality:
One female died on study Day 2
Clinical signs:
Piloerection, abnormal body carriage (hunched posture, abnormal gait (waddling), lethargy, decreased respiratory rate, partially closed eyelids, apllor of extremities, increased salivation, noisy respiration and walking on toes. Recovery was complete by Day 4.
Body weight:
Bodyweight gain was as expected for all animals surviving treatment.
Gross pathology:
No abnormalities noted at macroscopic examination.

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
The LD50 was found to be > 2.0 g/kg bw.
Executive summary:

Following a single oral dose of 2.0 g/kg bw test material, the LD50 was found to be > 2.0 g/kg bw.