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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1990-07-26 - 2017-07-27
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
minor deficiencies in documentation, no analytical verification of the test item concentrations; exposure duration only 24 h

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: "Determination of immobility of water flea -Daphnia magna-"
Version / remarks:
"Bestimmung der Schwimmunfähigkeit beim Wasserfloh -Daphnia magna-" (EC 0 , EC 50 , EC 100 ; 24 Stunden; statisches System), Verfahrensvorschlag: Umweltbundesamt Berlin, Stand Mai 1984
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
[8,20-dihydro-8,20-diphenyl-5,24:12,17-diimino-7,10:22,19-dinitrilodibenz[f,p][1,2,4,9,11,12,14,19]octaazacycloicosinato(2-)-N25,N26,N27,N28]iron
EC Number:
256-526-8
EC Name:
[8,20-dihydro-8,20-diphenyl-5,24:12,17-diimino-7,10:22,19-dinitrilodibenz[f,p][1,2,4,9,11,12,14,19]octaazacycloicosinato(2-)-N25,N26,N27,N28]iron
Cas Number:
50293-39-5
Molecular formula:
C32H18FeN12
IUPAC Name:
[8,20-dihydro-8,20-diphenyl-5,24:12,17-diimino-7,10:22,19-dinitrilodibenz[f,p][1,2,4,9,11,12,14,19]octaazacycloicosinato(2-)-N25,N26,N27,N28]iron
Test material form:
solid

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Remarks:
stock solution in medium
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Application in test medium as stock solution
- Controls: synthetic dilution water acc. Elendt
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): none stated

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Strain/clone: Daphnia magna Straus, parthenogenetic females, by Klonzucht des Bundesgesundheitsamtes Berlin
- Source: in-house breeding
- Age during test: 6-24 h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Post exposure observation period:
none stated

Test conditions

Test temperature:
19.3 - 19.5 °C
pH:
8.0
Dissolved oxygen:
8.9 - 9.2 mg/l
Nominal and measured concentrations:
Nominal: 0, 3.2, 10, 31.6, 100, 316, 1000 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: cylindric, diameter 4.0 cm, hight: 6.5 cm
- Material, size, headspace, fill volume: 20 ml test medium
- Aeration: not aerated
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
synthetic dilution water acc. Elendt

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen, pH, temperature, number of immobilized daphnids after 24h
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
ca. 316 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: not determined by calculation (Probit analysis)
Remarks:
10 of 20 daphnids were immobilized at this concentration
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
31.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
not determinable
Remarks:
highest concentration only resulted in 65% immobility
Details on results:
- Behavioural abnormalities: none stated
- Other biological observations: none stated
- Mortality of control: none stated
- Other adverse effects control: none stated
- Abnormal responses: none stated
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: yes
- Effect concentrations exceeding solubility of substance in test medium: no details
Results with reference substance (positive control):
- Relevant effect levels: EC0 = 1.0 mg/l, EC100 = 5.0 mg/l, EC50 = 2.6 mg/l
- Limit test: no
- Dose-response test: yes
- Other: The susceptibility of the laboratories' in-house population of Daphnia magna to the reference substance potassium dichromate showed noticeable drift phenomena in the past years. The EC50 value is not within the concentration range of 0.9 - 1.9 mg/l as stipulated by the guideline.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The study was performed according to an UBA-Proposal for testing for the Determination of immobility of water flea, and can hence be considered in general reasonable. No analytical dose verification however was performed. Due to the shortened exposure duration (24h), the test performance is however not designed as required for classification according to Regulation 1272/2008. In this study, the following EC-values (Immobility, effective concentrations) were determined: EC100 > 1000 mg/L; EC50 = 316 mg/L; EC0 = 31.6 mg/L, which are way above the possible water solubility of the registered substance, however after only 24h. Nevertheless it can be reasonably assumed that additional 24h exposure duration would not have led to EC50 values below 100 mg/l, as at a nominal concentration of 1000 mg/l, only 65% of the daphnids were immobilized. The noted effects were above the water solubility (<11.2 µg/l) and trigger classification as Aquatic Chronic Cat. 4.
Executive summary:

The study was performed acc. "Bestimmung der Schwimmunfähigkeit beim Wasserfloh -Daphnia magna-" (EC 0 , EC 50 , EC 100 ; 24 Stunden; statisches System), Verfahrensvorschlag: Umweltbundesamt Berlin, Stand Mai 1984. No analytical dose verification was performed. Daphnia magna Straus were exposed to nominal concentrations of 0, 3.2, 10, 31.6, 100, 316, 1000 mg/l for 24h, static test design. The following EC-values (Immobility) were determined: EC100 > 1000 mg/L; EC50 = 316 mg/L; EC0 = 31.6 mg/L. The noted effects were above the water solubility (<11.2 µg/l) and trigger classification as Aquatic Chronic Cat. 4.