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EC number: 236-308-9 | CAS number: 13291-61-7
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test procedure according to international standard
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- GLP compliance:
- no
- Specific details on test material used for the study:
- Supplier: Sigma
Purity/content: analytical grade - Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 610 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test procedure according to international standard
- Justification for type of information:
- The substance tetrasodium ethylenediaminetetraacetate (CAS 64-02-8) is structurally similar to the substance trans-cyclohexane-1,2-dinitrilotetraacetic acid (CAS 13291-61-7), therefore used for the read-across.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- GLP compliance:
- no
- Specific details on test material used for the study:
- Supplier: Sigma
Purity/content: analytical grade - Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 610 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Referenceopen allclose all
EC50 (24h) calculated (confidence interval 95%):
| Test substance | EC50, 24 h (mg/l) | Confidence interval, 95% |
| EDTA | 610 | 570 -640 |
| Mn(II)+EDTA | 940 | 800 -1000 |
| Fe(III)+EDTA | 17 | 13 -21 |
| Cu(II)+EDTA | 38 | 31 -45 |
| Zn(II)+EDTA | 910 | 840 -980 |
| Hg(II)+EDTA | 0.00032 | 0.00022 -0.00052 |
| Cd(II)+EDTA | 310 | 290 -330 |
All the EC50 values, except those obtained for the free EDTA, are expressed as mg/L of total amount of the studied metal.To compare the toxicities of the metal chelates with those of the free EDTA, all the EC50 values were converted to mg/L EDTA.
EC
50 values with 95% confidence limits were calculated by a probit analysis
computer program, version 2.3 (National Swedish Environmental Protection
Agency).
The EC50 values of Fe3+, Cu2+, Mn2+, Zn2+, Cd2+ and Hg2+ complexed by
EDTA were compared with those of the respective uncomplexed metals and
free EDTA.
The experiments gave the following toxicity order: Hg2+, Cu2+, Cd2+,
Zn2+, Fe3+, Mn2+ (higher to lower toxicity).
After complexation, the toxicities were 1.1-1700 (EDTA) times lower.
With Hg2+ complexation did not decrease the toxicity. Compared with the
toxicity of free EDTA, metal complexation increased the toxicity of EDTA.
iiibhbhhEC50(24h) calculated (confidence interval 95 %):
----------------------------------------------------
EDTA 610 mg/l (570-640)
Mn(II)+EDTA 940 mg/l (800-1000)
Fe(III)+EDTA 17 mg/l (13-21)
Cu(II)+EDTA 38 mg/l (31-45)
Zn(II)+EDTA 910 mg/l (840-980)
Hg(II)+EDTA 0.00032 mg/l (0.00022-0.00052)
Cd(II)+EDTA 310 mg/l (290-330)
----------------------------------------------------
All the EC50 values, except those obtained for the free EDTA, are expressed as mg/L of total amount of the studied metal.To compare the toxicities of the metal chelates with those of the free EDTA, all the EC50 values were con
EC50 (24h) calculated (confidence interval 95%):
| Test substance | EC50, 24 h (mg/l) | Confidence interval, 95% |
| EDTA | 610 | 570 -640 |
| Mn(II)+EDTA | 940 | 800 -1000 |
| Fe(III)+EDTA | 17 | 13 -21 |
| Cu(II)+EDTA | 38 | 31 -45 |
| Zn(II)+EDTA | 910 | 840 -980 |
| Hg(II)+EDTA | 0.00032 | 0.00022 -0.00052 |
| Cd(II)+EDTA | 310 | 290 -330 |
All the EC50 values, except those obtained for the free EDTA, are expressed as mg/L of total amount of the studied metal.To compare the toxicities of the metal chelates with those of the free EDTA, all the EC50 values were converted to mg/L EDTA.
EC
50 values with 95% confidence limits were calculated by a probit analysis
computer program, version 2.3 (National Swedish Environmental Protection
Agency).
The EC50 values of Fe3+, Cu2+, Mn2+, Zn2+, Cd2+ and Hg2+ complexed by
EDTA were compared with those of the respective uncomplexed metals and
free EDTA.
The experiments gave the following toxicity order: Hg2+, Cu2+, Cd2+,
Zn2+, Fe3+, Mn2+ (higher to lower toxicity).
After complexation, the toxicities were 1.1-1700 (EDTA) times lower.
With Hg2+ complexation did not decrease the toxicity. Compared with the
toxicity of free EDTA, metal complexation increased the toxicity of EDTA.
iiibhbhhEC50(24h) calculated (confidence interval 95 %):
----------------------------------------------------
EDTA 610 mg/l (570-640)
Mn(II)+EDTA 940 mg/l (800-1000)
Fe(III)+EDTA 17 mg/l (13-21)
Cu(II)+EDTA 38 mg/l (31-45)
Zn(II)+EDTA 910 mg/l (840-980)
Hg(II)+EDTA 0.00032 mg/l (0.00022-0.00052)
Cd(II)+EDTA 310 mg/l (290-330)
----------------------------------------------------
All the EC50 values, except those obtained for the free EDTA, are expressed as mg/L of total amount of the studied metal.To compare the toxicities of the metal chelates with those of the free EDTA, all the EC50 values were con
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 610 mg/L
Additional information
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