1,3,5-triisopropylbenzene

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Mar 3, 2015 to Jul 17, 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Version / remarks:

1984

Qualifier:

according to guideline

Guideline:

other: HJ/T 153-2004. The guidelines for the testing of chemicals [S]. Beijing: State Environmental Protection Administration, 2004

Qualifier:

according to guideline

Guideline:

other: CRC-MEP. The Guidelines for the Testing of Chemicals-Effects on Biotic Systems. [M]. The second edition. Beijing: China Environment Press. 2013: 58-62

Qualifier:

according to guideline

Guideline:

other: GBff 21809-2008, Chemicals-Test method of earthworm acute toxicity test [S]. Beijing: Standardization Administration of China, 2008

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)

Version / remarks:

2008

GLP compliance:

yes

Remarks:

According to the guidelines of chemical testing good laboratory practices in China

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Batch No. 6Fl 1027000
- Expiration date of the lot/batch: 2 years
- Purity: 96%

Analytical monitoring:

no

Vehicle:

yes

Remarks:

Acetone (in the definitive test)

Details on preparation and application of test substrate:

- Method of mixing into soil:
FOR THE RANGE FINDING TEST: the test substance was directly added into the soil. A total amount of 10 g mixture of the test substance and quartz sand was prepared firstly and then mixed with 550 g dry weight of soil for each test container. The medium was thoroughly mixed and deionised water was added finally to give an overall moisture content of about 35% dw.
FOR THE DEFINITIVE TEST: A stock solution was prepared be dissolving 5.2088 g test substance into 50.0 mL acetone. Test substrates at different concentrations were prepared by adding the stock solution to 10 g quartz sand and mixed. After evaporating the acetone at the room temperature, 10 g quartz sand then mixed with 550 g dry weight of soil for each test container. The medium was thoroughly mixed and deionised water was added finally to give an overall moisture content of about 35% dw.
- Evaporation of vehicle before use: For the definitive test, the acetone was evaporated at room temperature

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Source: An earthworm nursery of Nanjing city in China (Batch No.: E20150327E).
- Age at test initiation (mean and range, SD): Not specified
- Weight at test initiation (mean and range, SD): Range of average weight was 332-421 mg

ACCLIMATION
- Acclimation period: before the test, worms were acclimated in test soil for 17 days. Worms were then pre-cultured in test soils for another 24hrs. Worms were washed quickly with distilled water and surplus water absorbed on filter paper before use
- Acclimation conditions (same as test or not): Not reported (Noting that worms were acclimated in test soils for 24hrs)
- Health during acclimation (any mortality observed): Not specified

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Remarks:

14 days duration for range finding test and 14 days duration for definitive test

Post exposure observation period:

Mortality assessed on 7d and 14 d; number and type of behavioural and other pathological abnormalities (e.g. lethargic or rigid worms) were determined during each mortality assessment at 7d and 14 d.

Test temperature:

20.5°C to 21.8°C

pH:

6.10 - 6.39

Moisture:

27% to 35%

Details on test conditions:

TEST SYSTEM
- Amount of soil or substrate: dry weight: 560 g each
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: For the definitive test, 4 replications for each concentration level
- No. of replicates per control: 4
- No. of replicates per solvent control: 4

SOURCE AND PROPERTIES OF SUBSTRATE
- Artificial soil:
10% sphagnum peat
20% kaolin clay
70% quartz sand

OTHER TEST CONDITIONS
- Photoperiod: continuous light
- Light intensity: 400 lux to 800 lux

EFFECT PARAMETERS MEASURED: mortality and behavioral effects; observations taken at 7 and 14 days

VEHICLE CONTROL PERFORMED: Yes (solvent control)

TEST CONCENTRATIONS
- Definitive test concentrations: 100, 150, 225, 338, 506 mg/kg artificial soil (dry weight of soil)
- Spacing factor for test concentrations: 1.5

- Range finding study
- Test concentrations: 100, 500 and 1000 mg/kg artificial soil (dry weight)
- Results used to determine the conditions for the definitive study: Yes

Nominal and measured concentrations:

100, 150, 225, 338, 506 mg/kg artificial soil (dry weight of soil)

Reference substance (positive control):

yes

Remarks:

2-Chloracetamide, CAS: 79-07-2; purity: 2: 98.5%; Lot number: 20130905; Sinopharm Chemical Reagent Co. Ltd.

Key result

Duration:

7 d

Dose descriptor:

LC50

Effect conc.:

184 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% confidence 174-194 mg/kg dw soil

Duration:

7 d

Dose descriptor:

NOEC

Effect conc.:

100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

150 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% confidence 141 to 160 mg/kg dw soil

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Results with reference substance (positive control):

The 7-days LC50 and 14-days LC50 for 2-chloracetamide were 29.3 mg/kg and 21.2 mg/kg, respectively.

The 7-day and 14-day LC50 values for 2-chloracetamide are determined 3 times a year to assure that the laboratory test conditions are adequate and had not changed significantly.

Reported statistics and error estimates:

The LC50 and 95% confidence intervals were used to calculate by Trimmed Spearman-Karber Method (Version 1.5, USEPA).

Summary of validation criteria:

(1)         Control group

A blank control group, comprising the same number of test worms as each treatment, was also treated with the same methods while without using the test substance.

(2)         Reference control

The 7-day and 14-day LC50 values of a reference substance (2-chloracetamide) were determined 3 times a year to assure that the laboratory test conditions were adequate and had not changed significantly. The 7-days LC50 and 14-days LC50 for reference substance were 29.3 mg/kg and 21.2 mg/kg, respectively.

(3)         Validity of test result

Mortality in the untreated control group at the end of the study must not exceed 10%. Otherwise the test should be considered invalid.

Validity criteria fulfilled:

yes

Conclusions:

The test was conducted to determine the acute toxicity of the test item to earthworm. Based on the result of range-finding test and definitive test, the 7-d and 14-d LC50 of the test item in artificial soil were determined to be 184 mg/kg dw soil and 150 mg/kg dw soil, respectively. The highest concentration causing no mortality 7 day and 14 day LC0 were 100 mg/kg dry weight soil.

Executive summary:

The acute toxicity of 1,3,4-triisopropylbenzen was tested under static conditions in a 14-day toxicity study on the earthworm (Eisenia fetida) in a study similar to OECD TG 207. Based on nominal concentrations, the 7-day LC50 was determined to be 184 mg/L with a NOEC of 100 mg/L based on mortality. The 14-day nominal LC50 was determined to be 150 mg/L with a NOEC of 100 mg/L based on mortality.

Description of key information

The acute toxicity of 1,3,4-triisopropylbenzen was tested under static conditions in a 14-day toxicity study on the earthworm (Eisenia fetida) in a study similar to OECD TG 207. Based on nominal concentrations, the 7-day LC50 was determined to be 184 mg/L with a NOEC of 100 mg/L based on mortality. The 14-day nominal LC50 was determined to be 150 mg/L with a NOEC of 100 mg/L based on mortality.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

150 mg/kg soil dw