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Environmental fate & pathways

Hydrolysis

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Reference
Endpoint:
hydrolysis
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2007-03-19 to 2007-06-20
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
Version / remarks:
adopted December 29, 1992
Deviations:
no
GLP compliance:
yes (incl. certificate)
Radiolabelling:
no
Analytical monitoring:
yes
Details on sampling:
- Sampling intervals for the parent/transformation products: 0 hrs, 2.4 hrs and 5 days
- Sampling intervals/times for pH measurements: 0 hrs, 2.4 hrs and 5 days
Buffers:
- pH: 4
- Composition of buffer: 16.6% 0.05 M sodium acetate and 83.4% 0.05 M acetic acid

- pH: 7
- Composition of buffer: solution of 0.05 M potassium dihydrogenphosphate adjusted to pH 7 using 1 N sodium hydroxide

- pH: 9
- Composition of buffer: solution of 0.05 M boric acid and 0.05 M potassium chloride adjusted to pH 9 using 1 N sodium hydroxide
Details on test conditions:
TEST SYSTEM
- Type, material and volume of test flasks, other equipment used: sealed glass vessels
- Sterilisation method: filter-sterilisation (0.2 µm)
- Lighting: no data
- Measures to exclude oxygen: nitrogen gas was purged through the solutions for 5 minutes
- If no traps were used, is the test system closed/open: closed


Duration:
5 d
pH:
4
Temp.:
50 °C
Initial conc. measured:
1 095 mg/L
Duration:
5 d
pH:
7
Temp.:
50 °C
Initial conc. measured:
1 181 mg/L
Duration:
5 d
pH:
9
Temp.:
50 °C
Initial conc. measured:
1 054 mg/L
Number of replicates:
1
Positive controls:
no
Negative controls:
no
Preliminary study:
In a preliminary test the decrease in concentration of the test substance was measured at pH 4.0, 7.0 and 9.0. Since the decrease in concentration was below 10% after 5 days, no further testing is necessary.
Transformation products:
no
% Recovery:
103
pH:
4
Temp.:
25 °C
Duration:
2.4 h
% Recovery:
96.5
pH:
4
Temp.:
25 °C
Duration:
5 d
% Recovery:
101
pH:
7.1
Temp.:
25 °C
Duration:
2.4 h
% Recovery:
96.1
pH:
7.1
Temp.:
25 °C
Duration:
5 d
% Recovery:
104
pH:
8.9
Temp.:
25 °C
Duration:
2.4 h
% Recovery:
98.1
pH:
8.9
Temp.:
25 °C
Duration:
5 d
Key result
pH:
4
Temp.:
25 °C
DT50:
> 1 yr
Type:
not specified
Key result
pH:
7
Temp.:
25 °C
DT50:
> 1 yr
Type:
not specified
Key result
pH:
9
Temp.:
25 °C
DT50:
> 1 yr
Type:
not specified
Validity criteria fulfilled:
yes
Conclusions:
The half-life of Amphopropionate C8 at 25°C, pH 4, 7 and 9 is >1 year.
Executive summary:

The hydrolysis of Amphopropionate C8 at 1000 mg a.i./L was studied at 50ºC in sterile aqueous buffered solutions at pH 4 (acetate buffer), pH 7 (phosphate buffer) and pH 9 (borate buffer) for 5 days.  The experiment was conducted in accordance with EU method C.7 (1992). Samples were analysed by HPLC at 0 h, 2.4 h and 5 d.  In a preliminary test the decrease in concentration of the test substance was measured at pH 4.0, 7.0 and 9.0. Since the decrease in concentration was below 10% after 5 days, no further testing was necessary.

The half-life of Amphopropionate C8 at 25°C, pH 4, 7 and 9 is >1 year.

Description of key information

half-life 25°C, pH 4, 7 and 9 >1 year (EU method C.7; RL1; GLP)

Key value for chemical safety assessment

Additional information

The hydrolysis of Amphopropionate C8 at 1000 mg a.i./L was studied at 50ºC in sterile aqueous buffered solutions at pH 4 (acetate buffer), pH 7 (phosphate buffer) and pH 9 (borate buffer) for 5 days.  The experiment was conducted in accordance with EU method C.7 (1992). Samples were analysed by HPLC at 0 h, 2.4 h and 5 d.  In a preliminary test the decrease in concentration of the test substance was measured at pH 4.0, 7.0 and 9.0. Since the decrease in concentration was below 10% after 5 days, no further testing was necessary.

The half-life of Amphopropionate C8 at 25°C, pH 4, 7 and 9 is >1 year.