Registration Dossier

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

72 h ErC10 = 0.62 mg a.i./L (95% c.i. 0.32 - 1.21 a.i. mg/L); 72 h ErC50 65 mg a.i./L (95% c.i. 42-99 mg/L (OECD TG 201; Pseudokirchneriella subcapitata; RL1; GLP)

Not readily biodegradable

 

no classification for aquatic acute; Aquatic Chronic 2

Additional information

Short-term toxicity to fish

The 96 h LC50 of Amphopropionate C8 (50.6% a.i.) to carp (Cyprinus carpio) was > 100 mg a.i./L.  The EC50 and NOEC values, based on mortality/sublethal effects, were both > 100 mg a.i./L.  No visible sublethal effects were observed under the conditions of the present test. 

 

Short-term toxicity to aquatic invertebrates

The 48-hr-acute toxicity of Amphopropionate C8 (50.6% a.i.) to Daphnia magna was studied under static conditions accordign to OECD Guideline 202.  Daphnids were exposed to control and test chemical at nominal concentrations of 0 (control), 0.10, 1.0, 10, 100 mg a.i./L for 48 h.  Mortality and immobilisation were observed daily. 

Analysis of the samples taken during the combined limit/range-finding test showed that measured concentrations were stable and in agreement with nominal throughout the 48-hour test period (94-97%). Under the conditions of the present study Amphopropionate C8 did not induce acute immobilisation of Daphnia magna at or below 100 mg a.i./L, after 48 hours of exposure.

 

Toxicity to aquatic algae and cyanobacteria

In a 72 hour toxicity study, the cultures of Pseudokirchneriella subcapitata, strain NIVA CHL 1 were exposed to Amphopropionate C8 (50.6% a.i.) at nominal concentrations of 0 (control), 4.6, 10, 22, 46, 100 and 220 mg/L under static conditions in accordance with OECD guideline 201 (adopted March 23, 2006) and EU Method C.3 (1992). Analysis of the samples taken during the final test showed that measured concentrations were stable and in agreement with nominal throughout the 72-hour test period (90-97%). The lowest test group showed a slightly lower recovery between 82 and 86% relative to nominal.

The NOEC, EC10 and EC50 of Amphopropionate C8 to algae based on growth rate were < 4.6 mg a.i./L, 0.62 mg a.i./L (95% c.i. 0.32 - 1.21 a.i. mg/L) and 65 mg a.i./L (95% c.i. 42-99 mg/L). The NOEC and EC50 of Amphopropionate C8 to algae based on cell yield were < 4.6 mg a.i./L and 5.2 mg a.i./L (95% c.i. 4.7-5.7 mg/L).

  

Toxicity to microorganisms

In a 3 hour toxicity study conducted according to OECD Guideline 209 (1984) and EU Method C.11 (1988), the cultures of activated sludge of a predominantly domestic sewage treatment plant were exposed to Amphopropionate C8 (50.6% a.i.) at nominal concentrations of 0 (control) and100 mg a.i./L mg/L under static conditions.

There was no inhibitory effect of the test item at the limit concentration of 100 mg a.i./L.

 

Conclusion

The most sensitive organism was algae. Thus, the 72 h ErC50 of 65 mg/L is used to derive the PNECs.

Based on acute toxicity values >1 mg/L, no classification for aquatic acute toxicity is required according to GHS Regulation EC No 1272/2008.

Chronic toxicity values between 0.1 and 1 mg/L were obtained with algae. Acute toxicity values for fish and crustaceae were >/= 100 mg/L. And the substance is not readily biodegradable. Thus, according to GHS Regulation EC No 1272/2008, Amphopropionate C8 is classified as Aquatic Chronic 2.