Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 276-339-5 | CAS number: 72102-40-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From October 24, 2017 to October 27, 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- not applicable
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Deviations:
- not applicable
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: EPA-600-4-91-002 (Short-term methods for estimating the chronic toxicity of effects and receiving water to freshwater organisms)
- Version / remarks:
- 1994
- Deviations:
- not applicable
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- At the start of the test additional sacrificial vessels with dispensed test solution were taken for whole sample analysis and at the end of the test, replicate vessels of the dilution water control and each test concentration were taken for whole sample analysis.
- Vehicle:
- yes
- Remarks:
- Elendt's M4 culture medium
- Details on test solutions:
- Preparation of test solutions
The study was run with a dilution water control and nominal test substance exposure concentrations of 0.0625, 0.125, 0.25, 0.5 and 1.0 mg/L. A primary stock concentrate of test substance, with a nominal concentration of 10 mg/L, was prepared by adding a nominal 0.01 g of test substance to 1000 mL of culture medium. The resultant stock was observed to be clear and colourless with bubbles present on the surface and was used to prepare the test solutions. This was achieved by the direct addition of the appropriate amount of concentrate to dilution water in a volumetric flask. The control consisted of culture medium only. All test solutions were clear and colourless. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Test organism
The test organism was the fresh water crustacean, Daphnia magna (<24 h old), obtained from continuous laboratory cultures held at Scymaris. The stock cultures of D. magna were maintained in a reconstituted water medium, the same as the test dilution water, at a temperature of 20 ± 2°C. The cultures were maintained in 2 L glass vessels with a working volume of 1.6 L. A photoperiod of 16 h’ light:8 h dark, with 20-minute transition periods was provided. The D. magna cultures were fed on a mixed algae diet of Chlorella vulgaris, strain CCAP 211/12 and Pseudokirchneriella subcapitata, strain CCAP 278/4. The D. magna cultures were fed daily ad libitum depending on age and density of the culture. Culture conditions were such that the D. magna reproduction was by diploid parthenogenesis. D. magna <24 h old, obtained from two culture vessels, were used for testing. The parent animals were 14 ± 1 d old and had been maintained with a twice weekly renewal of reconstituted water medium since birth. The test organisms and the cultures from which they were obtained showed no evidence of disease before the test period. - Test type:
- static
- Water media type:
- other: Elendt's M4 D. magna medium
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- Total hardness as CaCO3 (mg/L): 214.7
- Test temperature:
- 20 ± 1°C
- pH:
- 7.89 to 8.12
- Dissolved oxygen:
- 8.99 to 9.22 mg/L
- Conductivity:
- Conductivity (µS/cm): 559
- Nominal and measured concentrations:
- 1 mg/L highest concentration (Based on non-GLP range-finding study)
0, 0.0625, 0.125, 0.25, 0.5 and 1.0 mg/L (nominal)
0, 0.047, 0.11, 0.21, 0.42 and 0.79 mg/L (measured) - Details on test conditions:
- Apparatus
Glass beakers of 250 mL nominal capacity were used as test vessels, with four replicates per test concentration. Each vessel contained 200 mL of test solution providing a depth of approximately 60 mm. The beakers were covered with loose fitting glass lids. The positions of the treatments were randomly allocated within the test area. - Reference substance (positive control):
- no
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.79 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Remarks on result:
- other: >0.758 mg/L a.i.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 0.56 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% Confidence interval: 0.48 - 0.62
- Remarks:
- 0.538 a.i.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 0.42 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Remarks on result:
- other: 0.403 mg/L a.i.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 0.79 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Remarks on result:
- other: 0.758 mg/L a.i.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the 48 h EC50 of test substance was determined to be 0.56 mg/L (0.538 mg a.i./L).
- Executive summary:
A study was conducted to determine the acute toxicity of the test substance, 'iso and anteiso C10-40 AAP EDM-ES' (active: 96%) to Daphnia magna, according to the OECD Guideline 202 method, in compliance with GLP. A 1 mg/L test substance concentration was selected as the highest concentration based on the toxicity demonstrated in a non-GLP range-finding study. Twenty test organisms (four replicates per test concentration, 5 daphnids per test vessel, i.e., 20 daphnids per test concentration) were exposed to each nominal test substance concentrations of 0, 0.0625, 0.125, 0.25, 0.5 and 1.0 mg/L for 48 h under static conditions. The concentrations of test substance in the test vessels were measured using the high-performance liquid chromatography method. The mean measured concentrations of test substance were determined to be 0, 0.047, 0.11, 0.21, 0.42 and 0.79 mg/L. Immobilisation was recorded at 24 and 48 h and compared with control values. Based on immobility compared to the control (p <0.05), the 48 h No Observed Effect Concentration (NOEC) was determined to be 0.42 mg/L (measured) and the Lowest Observed Effect Concentration (LOEC) was 0.79 mg/L (0.758 mg/L a.i.). No other symptoms of toxicity were observed. Based on the study results, the 48 h EC50 was calculated to be 0.56 mg/L (0.538 mg a.i./L). As 5% immobility and no other signs of stress were observed in the control and dissolved oxygen concentration remained at ≥3 mg/L, the test was considered to have satisfied all the validity criteria. Under the study conditions, the 48 h EC50 of the test substance, 'iso and anteison C10-40 AAP EDM-ES' was determined to be 0.56 mg/L (0.538 mg a.i./L) (Scymaris, 2017).
Reference
Analytical data
The nominal and measured concentrations of test substance determined in the test solutions were 0, 0.0625, 0.125, 0.25, 0.5, 1.0 mg/L and 0, 0.047, 0.11, 0.21, 0.42, 0.79 mg/L respectively. The limit of quantification of test substance in this study was 0.02 mg/L. All analytical values were quoted to two significant figures and percentages to the nearest integer. The measured concentrations at the start of the study were 77-88% of nominal and at the end were 72-84% of nominal. On the basis of the analytical data the mean measured concentrations were used for the calculation and reporting of results.
Biological data
The results obtained (based on mean measured concentrations of test substance) were:
Time |
EC50 |
95% confidence limits |
Calculation method |
24 h |
>0.79 |
- |
ICPIN |
48 h |
0.56 |
0.48 – 0.62 |
ICPIN |
Based on immobility compared to the control (p <0.05), the 48 h No Observed Effect Concentration (NOEC) was determined to be 0.42 mg/L and the Lowest Observed Effect Concentration (LOEC) was 0.79 mg/L. There was one immobile D. magna observed in the dilution water control. One D. magna was on the surface of the test solution in 0.0625 mg/L Rep D and 0.125 mg/L Rep A at 48 h. No other symptoms of toxicity were observed.
Validity criteria
The OECD 202 Test Guideline details the following performance criteria for the test validity:
- In the control, no more than 10% of the daphnids should have been immobilised or show other signs of stress;
- The dissolved oxygen concentration at the end of the test should be ≥3 mg/L in control and test vessels.
As 5% immobility and no other signs of stress were observed in the control and dissolved oxygen concentration remained at ≥3 mg/L, this test has satisfied all validity criteria.
Description of key information
Based on the study results, the 48 h EC50 value of the test substance, 'iso and anteiso C10-40 AAP EDM-ES' for toxicity in Daphnia magna was determined to be 0.538 mg a.i./L (measured).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.538 mg/L
Additional information
A study was conducted to determine the acute toxicity of the test substance, 'iso and antesio C10-40 AAP EDM-ES' (active: 96%) to Daphnia magna, according to the OECD Guideline 202 method, in compliance with GLP. A 1 mg/L test substance concentration was selected as the highest concentration based on the toxicity demonstrated in a non-GLP range-finding study. Twenty test organisms (four replicates per test concentration, 5 daphnids per test vessel, i.e., 20 daphnids per test concentration) were exposed to each nominal test substance concentrations of 0, 0.0625, 0.125, 0.25, 0.5 and 1.0 mg/L for 48 h under static conditions. The concentrations of test substance in the test vessels were measured using the high-performance liquid chromatography method. The mean measured concentrations of test substance were determined to be 0, 0.047, 0.11, 0.21, 0.42 and 0.79 mg/L. Immobilisation was recorded at 24 and 48 h and compared with control values. Based on immobility compared to the control (p <0.05), the 48 h No Observed Effect Concentration (NOEC) was determined to be 0.42 mg/L (measured) and the Lowest Observed Effect Concentration (LOEC) was 0.79 mg/L (0.758 mg/L a.i.). No other symptoms of toxicity were observed. Based on the study results, the 48 h EC50 was calculated to be 0.56 mg/L (0.538 mg a.i./L). As 5% immobility and no other signs of stress were observed in the control and dissolved oxygen concentration remained at ≥3 mg/L, the test was considered to have satisfied all the validity criteria. Under study conditions, the 48 h EC50 of the test substance, 'iso and antesio C10-40 AAP EDM-ES' was determined to be 0.56 mg/L (0.538 mg a.i./L) (Scymaris, 2017).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

EU Privacy Disclaimer
This website uses cookies to ensure you get the best experience on our websites.