Cyclopenta[b]pyrrole-2-carboxylic acid, octahydro-, phenylmethyl ester, hydrochloride (1:1), (2S,3aS,6aS)-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Cyclopenta[b]pyrrole-2-carboxylic acid, octahydro-, phenylmethyl ester, hydrochloride (1:1), (2S,3aS,6aS)-

EC Number:

617-991-6

Cas Number:

87269-87-2

Molecular formula:

C15 H19 N O2 * H Cl

IUPAC Name:

Cyclopenta[b]pyrrole-2-carboxylic acid, octahydro-, phenylmethyl ester, hydrochloride (1:1), (2S,3aS,6aS)-

Sampling and analysis

Analytical monitoring:

yes

Remarks:

HPLC-UV

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna, clone 5; 1.75 to 18.75 hours old.
The daphnids introduced in the test were taken from ibacon's in-house laboratory culture.

Study design

Test type:

semi-static

Water media type:

other: reconstituted water (Elendt "M4")

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

No information

Test conditions

Hardness:

2.5 mmol/L (= 250 mg/L) as CaCO3

Test temperature:

19 to 21°C (water temperature)

pH:

7.3 to 8.0

Dissolved oxygen:

8.6 to 9.0 mg/L

Nominal and measured concentrations:

Nominal: 100, 33.05, 11.1, 3.7, 1.2, 0.41 and 0.14 mg test item/L.
Measured: 51.8, 11.4, 2.95, 0.790, 0.184, 0.0679, 0.0326 mg test item/L.

Details on test conditions:

Photoperiod: 16h light - 8h dark.
Light intensity: 140 to 240 lux.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 2.95 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

ca. 11.4 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 17.2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

After 48 hours of exposure, no immobilisation of the test animals was observed in the control and the test item concentration up to and including 2.95 mg test item/L. At the concentration of 11.4 mg test item/L, 1 daphnid was immobile. At the highest concentration of 51.8 mg test item/L, all 20 daphnids were immobile. All concentrations refer to time weighted average measured concentrations.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on the experimental results, the 48-h NOEC was determined to be 2.95 mg test item/L. The 48-h LOEC was determined to be 11.4 mg test item/L and the 48-h EC50 value was calculated to be 17.2 mg test item/L.