Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
8 - 12 October 2001
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
Study according to test guideline OECD203 (not performed according to GLP). The test report is with detailed documentation. Further details on the test item are lacking in the report.
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
1992
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Version / remarks:
1992
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Static
- Controls: A blank of test medium without test substance or other additives served as a control.
- Concentration of vehicle in test medium: Nominal 100 mg/l was prepared by dilution of a stock solution or, in case of poorly soluble compounds, by adding the test substance direcily to the test medium.
Test organisms (species):
Poecilia reticulata
Details on test organisms:
TEST ORGANISM
- Common name: Guppy
- Source: BMG Ecolaboratories
- Age at study initiation (mean and range, SD): not determined
- Length at study initiation (length definition, mean, range and SD): 1-3 cm
- Weight at study initiation (mean and range, SD): not determined
- Method of breeding: Parental and young fish population was held in two 250 I glass aquaria
- Maintenance of the brood fish:
Medium: Continuously aerated and filtered tap water from Schlieren. The bottom of the aquaria was covered with gravel (<1,5 cm). Every month,
one third of the total volume of the aquaria was replaced by fresh tap water.
Feed: Sera micropan, Sera vipan (Sera apanristic GmbH, D-52525 Heinsberg, Germany) daily. The fish were last fed 24 h belore the start of the
definitive test.
Illumination: 14 h a day
Temperature: 23 +/- 2 °C
Health: During 7 days before the definitive test, mortality in the batch of which fish were used for the test was less than 5 %.

FEEDING DURING TEST: not specified
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
15.3 - 27.8 °F
Test temperature:
22 - 22.5 °C
pH:
8.2 - 8.5
Dissolved oxygen:
6.8 -7.2 mg/l
Conductivity:
510 - 585 uS/cm
Nominal and measured concentrations:
0 and 100 mg/l Nominal
Details on test conditions:
TEST SYSTEM
- Test vessel: 1 l aquaria, all-glass
- Aeration: aereated
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water from Schlieren, aerated

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light/day

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- only limit test 100 mg/l (nominal)
Key result
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
At the nominal concentration of 100 mg/l none of a total of seven fish died after 96h of exposure. No mortality was also observed in the blank control.
Other toxic effects than mortality, e.g. loss of coordination, hypo- or hyperactivity and swimming on the back, were not observed.
Reported statistics and error estimates:
Based on the binominal theory, the absence of mortality provides at least 99 % confidence that the LC50 is >100 mg/l.
Validity criteria fulfilled:
yes
Conclusions:
The no-observed-effect concentration (NOEC) was >=100 mg/l.
100 % mortality (LC100) could not be detected.
Based on the binominal theory, the absence of mortality provides at least 99 % confidence that the LC50 is greater than the applied nominal test concentration of 100 mg/l.
Executive summary:

The acute lethal toxicity of REVATOL NS to Poecitia reticulata (Guppy) was investigated under static exposure conditions over a period of 96 h. The only applied nominal test concentration was 100 mg/l.

No chemical analysis of the test substance concentration was conducted.

At the nominal concentrationof 100 mg/l none of a total of seven fish died after 96h of exposure. No mortality was also observed in the blank control.

Other toxic effects than mortality, e.g. loss of coordination, hypo- or hyperactivity and swimming on the back, were not observed. The no-observed-effect concentration (NOEC) was >=100 mg/l.

100 % mortality (LC100) could not be detected.

Based on the binominal theory, the absence of mortality provides at least 99 % confidence that the LC50 is greater than the applied nominal test concentration of 100 mg/l.

Description of key information

The no-observed-effect concentration (NOEC) was >=100 mg/l.

Based on the binominal theory, the absence of mortality provides at least 99 % confidence that the LC50 is greater than the applied nominal test concentration of 100 mg/l.

Key value for chemical safety assessment

Additional information