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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
not specified in report
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
secondary source
Title:
Unnamed
Year:
2000

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
434-600-2
EC Name:
-
Cas Number:
2628-16-2
Molecular formula:
CH3CO2C6H4CH=CH2
IUPAC Name:
4-ethenylphenyl acetate
Test material form:
liquid

Test solutions

Vehicle:
yes
Remarks:
acetone

Test organisms

Test organisms (species):
Daphnia magna

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
2.85 mg/L
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1.66 mg/L
Key result
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
5 mg/L
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.313 mg/L
Details on results:
% concentration loss over test: 100

Any other information on results incl. tables

Analysis of duplicate samples at the beginning and end of the test indicated that intended exposure concentrations of the test substance were achieved but not maintained (at all concentrations, maesured values after 48 hours were below the limit of detection).

At the lowest exposure concentration, 15% of the Daphia were mobile but swimming at the surface of the test medium. No sush floaters were observed at 0.313 mg/ml, but some animals in all exposure groups at higher concentrations were mobile and submerged.

The 48-hour median effect concentration (EC50) for immobilisation, based on nominal concentrations, was calculated to be 1.66 mg/ml (95% confidence limits of 1.35 and 2.11 mg/ml) based on the numbers of floating and/or immobile Daphnia, the 48-hour EC50 was found to be 1.39 mg/ml (95% confidence limits of 1.13 and 1.74 mg/ml)

The test material was found to be susceptible to hydrolysis, so intended exposure limits of the parent material could not be maintained during the test. However, the conditions employed are considered to reflect potential real-life exposure of Daphnia in surface waters following accidental release of the test substance.

Water hardness ranged from 218 to 232 mg CaCO3/1

Applicant's summary and conclusion