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EC number: 203-405-2 | CAS number: 106-51-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02.04.2018-27.04.2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- no
- Remarks:
- accredited by DAkkS
Test material
- Reference substance name:
- p-benzoquinone
- EC Number:
- 203-405-2
- EC Name:
- p-benzoquinone
- Cas Number:
- 106-51-4
- Molecular formula:
- C6H4O2
- IUPAC Name:
- 1,4-benzoquinone
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- not specified
- Details on test solutions:
- The test vessels contained a final volume of 10 mL (p-benzoquinone stock solution and dilution water). For each dilution stage and a control solution (contains only water) four 30 mL-test tubes with 5 daphniae were prepared. The test tubes were covered with Kapsenberg caps and left for 48 h. Incubation was performed at room temperature (20-22°C) in a 12 h/12 h light-dark rhythm without feed portion. The daphnia mobility was checked and documented after 24 h and 48 h.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna Straus
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20-22°C
- pH:
- 7,6 (+/-0,2)
- Dissolved oxygen:
- 8,5 (+/-0,2) in the beginning of the test
7,6 (+/-0,1) in the end of the test - Salinity:
- dilution water:
25 ml 0,08 M CaCl2-solution
+ 25 ml 0,02 M MgSO4-solution
+ 25 ml 0,03 M NaHCO3-solution
+ 25 ml 0,003 M KCl in 1 L deionised water - Conductivity:
- 606 μS/cm (dilution water)
- Nominal and measured concentrations:
- 0,05 mg/l, 0,10 mg/l, 0,15 mg/l, 0,20 mg/l, 0,25 mg/l
- Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- A daphnia is assessed as immobile provided that it did not move independently or after slight swinging 15 s as a minimum. Other effects of the test substance on daphniae like coloration or special stress behaviour were not observed.
Any other information on results incl. tables
The relevant concentration range for the calculation of the EC50-value was determined in preliminary tests.
Tab. 1: Mobility of Daphnia magna Straus at different p-benzoquinone concentrations
concentration [mg/l] |
Mobility of Daphnia magna after |
|||
24 h |
48 h |
|||
Amount |
% |
Amount |
% |
|
0,05 |
20 |
100 |
20 |
100 |
0,10 |
18 |
90 |
17 |
85 |
0,15 |
10 |
50 |
8 |
40 |
0,20 |
6 |
30 |
4 |
10 |
0,25 |
0 |
0 |
0 |
0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Reference samples did not fall below 90% daphnia mobility. Oxygen content at the test end was always above 3 mg/L.
- Conclusions:
- In a study according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test), a 48 h EC50 = 0,13 mg/L for p-benzoquinone was determined.
- Executive summary:
The acute toxicity of p-benzoquinone to Daphnia magna was investigated in a study according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) under static conditions. The endpoint examined was immobilization. The daphnids (5 animals/concentration) were exposed to nominal concentrations of 0,05 mg/l, 0,10 mg/l, 0,15 mg/l, 0,20 mg/l and 0,25 mg/l p-benzoquinone.
The 48 h EC50 was determined to be 0,13 mg/L based on nominal concentrations.
The testing/validity criteria laid down in the OECD guideline 202 are fulfilled. This study is regarded as reliable without restriction.
Thus, a 48 h EC50=0.13 mg/L (recalculated based on initial concentrations) has to be taken into account for the assessment.
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