Reaction mass of (2,4,6-trioxo-1,3,5-triazine-1,3,5(2H,4H,6H)-triyl)tri-2,1-ethanediyl triacrylate and 2-Propenoic acid, 1,1'-[[dihydro-5-(2-hydroxyethyl)-2,4,6-trioxo-1,3,5-triazine-1,3(2H,4H)-diyl]di-2,1-ethanediyl] ester

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Justification for type of information:

The hypothesis for a read-across approach to data from TMPTA is the similarity of the chemical structures.Both the target substance (T) and TMPTA are triacryaltes.

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

Qualifier:

according to guideline

Guideline:

other: OECD 301C

GLP compliance:

yes (incl. QA statement)

Remarks:

Only specified for the study following the OECD 301C Guideline

Key result

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

625 mg/L

Nominal / measured:

nominal

Conc. based on:

other: TMPTA

Basis for effect:

inhibition of total respiration

Remarks:

ISO 8192

Conclusions:

Trimethylolpropantriacrylate was tested for respiratory sludge inhibition according to guideline ISO/DIS 8192 Part B. The inoculum was derived from the local municipal STP Lambsheim. The EC20 was determined to be after 30 min EC20 = 625 mg/l.

The target is primarily a mixture of trimethylolpropantriacrylate and dimethylolpropantriacrylate, and these two acrylates are assessed to show the same level of environmental toxicity to aquatic organism - the triacrylate compound being only very slightly more toxic than the diacrylate (QSAR calculations confirm this). Therefore, it is asssessed valid to set to toxicity to microorganism of a mixture of trimethylolpropantriacrylate and dimethylolpropantriacrylate to the toxicity of trimethylolpropantriacrylate.

Based on the test result an inhibition of the respiration and biodegradation activities of mircroorganisms within an STP is not expected.

Executive summary:

Trimethylolpropantriacrylate was tested for respiratory sludge inhibition according to guideline ISO/DIS 8192 Part B. The inoculum was derived from the local municipal STP Lambsheim. The EC20 was determined to be after 30 min EC20 = 625 mg/l.

The target is primarily a mixture of trimethylolpropantriacrylate and dimethylolpropantriacrylate, and these two acrylates are assessed to show the same level of environmental toxicity to aquatic organism - the triacrylate compound being only very slightly more toxic than the diacrylate (QSAR calculations confirm this). Therefore, it is asssessed valid to set to toxicity to microorganism of a mixture of trimethylolpropantriacrylate and dimethylolpropantriacrylate to the toxicity of trimethylolpropantriacrylate.

Based on the test result an inhibition of the respiration and biodegradation activities of mircroorganisms within an STP is not expected.

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988-05-16

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

Deviations:

no

GLP compliance:

not specified

Analytical monitoring:

not specified

Test organisms (species):

activated sludge, domestic

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Key result

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

625 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

EC20 after 30 min EC20 = 625 mg/l

highest test conc. causing < 20 % respiratory inhibition: 467.6 mg/l

inoculum: municipal STP: Lambsheim

Validity criteria fulfilled:

yes

Conclusions:

The test substance cause after 30 min an EC20= 625 mg/l

Executive summary:

Trimethylolpropantriacrylat was tested for respiratory sludge inhibition according to guideline ISO/DIS 8192 Part B. The inoculum was derived from the local municipal STP Lambsheim. The EC20 was determined to be after 30 min EC20 = 625 mg/l.

Based on the test result an inhibition of the respiration and biodegradation activities of mircroorganisms within an STP is not expected.

Description of key information

The substance ( Trimethylolpropantriacrylate) was tested for respiratory sludge inhibition according to guideline ISO/DIS 8192 Part B. The study was assessed to be reliable with restrictions and considereed as key study. The inoculum was derived from the local municipal STP Lambsheim. The EC20 was determined to be after 30 min EC20 = 625 mg/l. This concentration is also considered to be the EC50.

Key value for chemical safety assessment

EC50 for microorganisms:

625 mg/L

Additional information

The substance was tested for respiratory sludge inhibition according to guideline ISO/DIS 8192 Part B. The study was assessed to be reliable with restrictions and considereed as key study. The inoculum was derived from the local municipal STP Lambsheim. The EC20 was determined to be after 30 min EC20 = 625 mg/l. This concentration is also considered to be the EC50.