Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 947-151-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: Testing Methods for New Chemical Substances (Nov 21, 2003, No.1121002, Pharmaceutical and Food Safety Bureau, MHLW; Nov 13, 2003 No.2, Manufacturing Industries Bureau, METI; No.031121002, Environmental Policy Bureau, Ministry of the Environment)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- (2006)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guidance Document No. 23 (Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures), 2000
- Deviations:
- not applicable
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: nominal 100 mg/L and control
- Sampling intervals: test start and end
- Sampling method:
test start: a sample was taken from the container for preparation;
test end: samples were taken from the test solution in the replicate vessels, mixed and centrifuged to remove algae
- Sample storage conditions before analysis: none - Vehicle:
- no
- Details on test solutions:
- The test material and dilution water were mixed to produce nominal 100 mg/L. The dispersions were stirred by magnetic stirrer for about 48 hours to prepare the test solution. Undissolved test material was not removed.
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- - Strain: ATCC 22662
- Source (laboratory, culture collection): laboratory culture, originating from a strain obtained 1995 from the American Type Culture Collection (ATCC)
- Age of inoculum (at test initiation): Three days. Algal cells used for inoculation were exponentially growing.
- Method of cultivation: The pre-culture was incubated under the same conditions as in the test.
ACCLIMATION
- Acclimation period: 3 days (pre-culturing)
- Culturing media and conditions (same as test or not): same - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Hardness:
- 25 mg/L as CaCO3
- Test temperature:
- 23.3-23.5 °C
- pH:
- test start (0 h): 7.8
test end (72 h): 8.2 - Nominal and measured concentrations:
- nominal: 100 mg/L
measured concentrations at test start and end: <0.022 mg/L (LOQ) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 mL Erlenmeyer flasks (sterilized), fill volume 100 mL, plugged with gas-permeable silicon rubber plugs
- Aeration/gaseous exchange: rotary shaker, continuously oscillating at approximately 100 rpm
- Initial cells density: 1x10⁴ cells/mL (corresponding to 1.8 relative chlorophyll fluorescence units)
- Control end cells density: ≥ 230 relative chlorophyll fluorescence units
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes (OECD TG 201)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: purified water was used to prepare the test medium
- Culture medium different from test medium: no
- Intervals of water quality measurement:
pH and appearance of the test solutions: start and end of the exposure
temperature and light intensity: once a day
OTHER TEST CONDITIONS
- Sterile test conditions: yes (sterilized test vessels, medium used under sterile conditions)
- Adjustment of pH: no
- Photoperiod: continuous illumination
- Light intensity and quality: 96-105 µE/m²/s, fluorescent light, wavelength range 400-700 nm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : chlorophyll fluorescence was measured at 24, 48 and 72 hours
- Determination of cell concentrations: spectrophotofluorometer
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: nominal 100 mg/L (prepared as for the main test).
- Results used to determine the conditions for the definitive study: no growth inhibition was observed - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no microscopic abnormalities
The test solution was a white suspension, slightly green due to algea at test end. - Results with reference substance (positive control):
- - 72-hour ErC50: 0.72 mg/L (normal range in this laboratory: 0.56 to 1.4 mg/L)
- Validity criteria fulfilled:
- yes
- Remarks:
- (Increase of biomass >16 after 72 hours; mean coefficient of variation for section-by-section specific growth rate <35%; coefficient of variation for average specific growth rate <7%.)
Reference
Description of key information
72-h ErC50 >100 mg/L, 72-h NOEC ≥ 100 mg/L (nominal) for Pseudokirchneriella subcapitata (OECD 201)
Key value for chemical safety assessment
Additional information
The test results indicate that the test material is not inhibitory to growth of algae up to the limits of its water solubility and a NOEC and thus a PNEC cannot be determined.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.