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Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

12.12.06 - 16.12.06

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: PARCOM 1995 Part B

Principles of method if other than guideline:

Test Validity Criteria
This test was judged to be valid according to the following criteria as stated in test guideline PARCOM
1995 Part B:
• The mOliality in the control did not exceed 10<% (or 1 fish if less than 10 are used) at the end of
the test.
• The dissolved oxygen concenh'ation was at least 60% of the air sahlration value throughout the
test.
• Constant conditions were maintained as far as possible throughout the test and, semi-static
procedures were used.
• MOliality after 96 h in the presence of a standard reference toxicant (2.3 mg r l 3,5,dichlorophenol)
was in the range 20 - 80%
2221

GLP compliance:

yes

Specific details on test material used for the study:

Test item name:
Batch/Ref. ID No.:
Manufacturer/Supplier:
Date test item received:
Study Ref. No.:
Dodicor V 5654
06-SK131-4
Clariant GmbH
12.02.07
2181/ fish

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Cyprinodon variegatus

Details on test organisms:

Immature juvenile sheepshead mitillow (Cyprinodon variegatlls) of a size 1.5 - 2.5 cm in length are
used. The fish are held in the test laboratory for at least 12 days before they are used for testing. For
the last 4 days before a test the fish must be held in water quality similar to test conditions:
Light regime: 12 - 16 hours photoperiod
Temperature: 18.0 - 22.0 °c
Oxygen: ::c: 60% saturation
The fish are not fed during the 24 hours before test start.

Test type:

static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

20 +/- 2.0 °C

pH:

Normal for seawater (not adjusted)

Dissolved oxygen:

> 60 & air saturation

Salinity:

46 -54 mS/cm / 30 -36 Promille

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

An acute limit test was performed on juvenile sheepshead minnow, Cyprinodon variegatus, using
Dodicor V 5654 at a nominal concentration of 100 mg/L.
96h-LC50 > 100 mg/L
96h-NOEC = 100 mg/L

Executive summary:

An acute limit test was performed on juvenile sheepshead minnow, Cyprinodon variegatus, using

Dodicor V 5654 at a nominal concentration of 100 mg/L. The following results were obtained:

96-h LC50 > 100 mg/L

24-h LC50 > 100 mg/L

48-h LC50 > 100 mg/L

72-h LC50 > 100 mg/L

96-h LC100 > 100 mg/L

96-hNOEC: 100 mg/L

Comments: Stock solutions of the test item were prepared according to procedure 0, as described in the

OSPAR Guidelines for Toxicity Testing of Substances and Preparations Used and

Discharged Offshore. See pages 10-11 of this repOli. Test solutions were prepared from the

Water Accommodated Fraction (WAF).

Note: test results obtained when the test item is prepared according to procedure A refer to

the nominal concentrations of the test item as stated. Test results obtained when the test item

is prepared according to procedures B, C or D refer only to Water-Accommodated Fractions

ofthe test item prepared at the nominal concentrations of the test item stated.

The test results presented in this report were obtained from tests performed on a sample of

the test item sent to the test facility of Clariant Oil Services, formulated according to the

specifications supplied by the manufacturer or supplier at the time of testing. The test

results cannot be assumed to be valid for samples resulting £i'om subsequent modifications

to the original specifications as supplied.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

20.03.07 - 24.03.07

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: PARCOM 2005 Part B

Principles of method if other than guideline:

Test Validity Criteria
This test was judged to be valid according to the following criteria as stated in test guideline PARCOM
2005 Part B:
• The mOliality in the control did not exceed 10<% (or 1 fish if less than 10 are used) at the end of
the test.
• The dissolved oxygen concenh'ation was at least 60% of the air sahlration value throughout the
test.
• Constant conditions were maintained as far as possible throughout the test and, semi-static
procedures were used.
• MOliality after 96 h in the presence of a standard reference toxicant (2.3 mg r l 3,5,dichlorophenol)
was in the range 20 - 80%
2221

GLP compliance:

yes

Specific details on test material used for the study:

Test item name:
Batch/Ref. ID No.:
Manufacturer/Supplier:
Date test item received:
Study Ref. No.:
Dodicor V 5654
07-YS-019
Clariant GmbH
12.02.07
2221/ fish

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Cyprinodon variegatus

Details on test organisms:

Immature juvenile sheepshead mitillow (Cyprinodon variegatlls) of a size 1.5 - 2.5 cm in length are
used. The fish are held in the test laboratory for at least 12 days before they are used for testing. For
the last 4 days before a test the fish must be held in water quality similar to test conditions:
Light regime: 12 - 16 hours photoperiod
Temperature: 18.0 - 22.0 °c
Oxygen: ::c: 60% saturation
The fish are not fed during the 24 hours before test start.

Test type:

static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

20 +/- 2.0 °C

pH:

Normal for seawater (not adjusted)

Dissolved oxygen:

> 60 & air saturation

Salinity:

46 -54 mS/cm / 30 -36 Promille

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

An acute limit test was performed on juvenile sheepshead minnow, Cyprinodon variegatus, using
Dodicor V 5654 at a nominal concentration of 100 mg/L.
96h-LC50 > 100 mg/L
96h-NOEC = 100 mg/L

Executive summary:

An acute limit test was performed on juvenile sheepshead minnow, Cyprinodon variegatus, using

Dodicor V 5654 at a nominal concentration of 100 mg/L. The following results were obtained:

96-h LC50 > 100 mg/L

24-h LC50 > 100 mg/L

48-h LC50 > 100 mg/L

72-h LC50 > 100 mg/L

96-h LC100 > 100 mg/L

96-hNOEC: 100 mg/L

Comments: Stock solutions of the test item were prepared according to procedure 0, as described in the

OSPAR Guidelines for Toxicity Testing of Substances and Preparations Used and

Discharged Offshore. See pages 10-11 of this repOli. Test solutions were prepared from the

Water Accommodated Fraction (WAF).

Note: test results obtained when the test item is prepared according to procedure A refer to

the nominal concentrations of the test item as stated. Test results obtained when the test item

is prepared according to procedures B, C or D refer only to Water-Accommodated Fractions

ofthe test item prepared at the nominal concentrations of the test item stated.

The test results presented in this report were obtained from tests performed on a sample of

the test item sent to the test facility of Clariant Oil Services, formulated according to the

specifications supplied by the manufacturer or supplier at the time of testing. The test

results cannot be assumed to be valid for samples resulting £i'om subsequent modifications

to the original specifications as supplied.

Description of key information

2 Limit Tests with Cyprinodon variegatus are available. All tests shown that the 96 -NOEC is at 100 mg/L and the 96 -h LC50 is > 100 mg/L.

All test are done according OSPARCOM test Guidelines in seawater.