Dodecyltrimethylammonium bromide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

other: Publication in literature

Adequacy of study:

other information

Study period:

1999

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

Data considered acceptable for purpose of classification of an intermediate substance under SCC.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

other: Publication in literature

Adequacy of study:

other information

Study period:

1999

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

Data considered acceptable for purpose of classification of an intermediate substance under SCC.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

not indicated

Deviations:

yes

Principles of method if other than guideline:

- Daphnias were observed for 24 hours instead of 48 hours.
- No analytics of the test concentrations were reported.

GLP compliance:

no

Remarks:

Publication in literature

Analytical monitoring:

not specified

Vehicle:

no

Details on test solutions:

The test substance was dissolved in Standard Reference Water (SRW) or distilled water.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia
- Source: Department of Biology, University of Turku
- Age at study initiation: <24 hours
- Feeding during test: no

ACCLIMATION: not specified

CULTURE CONDITIONS
- Medium: M4
- Temperature: 18-20°C
- Photperiod (hr light/hr dark): 12/12
- Feeding: yes, Scenedesmus algae, every second or third day depending on the density of the culture

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Remarks on exposure duration:

An exposure period of 24 hours was determined based on the knowledge that toxicity of surfactants is usually expressed within the first 24 hours.

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL polystyrene vessels, fill volume: 50 mL, closed with a polyethylene cap
- Aeration: no
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Standard Reference Water (SRW) according to OECD guideline.
- Culture medium different from test medium: yes
- Intervals of water quality measurement: not specified

OTHER TEST CONDITIONS: not specified

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilization was determined visually after 24 hours

Reference substance (positive control):

not specified

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

0.37 mg/L

Nominal / measured:

not specified

Basis for effect:

mobility

Details on results:

- Results were expressed in micromolar: EC50 = 1.2 µM
- No abnormalities during the observations of the Daphnia were reported.
- No details were provided concerning the basis of the effect levels. Since also no analytics were reported, it is assumed that the effect level was based on the nominal concentrations.

Validity criteria fulfilled:

not specified

Conclusions:

The EC50 for Dodecyltrimethylammonium bromide towards Daphnia magna was 0.37 mg/L.

Executive summary:

The acute toxicity of Dodecyltrimethylammonium bromide towards Daphnia magna was assessed in a study available in public literature, performed similar to OECD guideline 202. Deviating from the guideline was the shorter exposure period (24 hours) based on the knowledge that toxicity of surfactants is usually expressed within the first 24 hours. Also, test solutions were not analysed for actual exposure concentrations. A range of five different concentrations was tested and a control was included. Twenty daphnids (<24 hours) were exposed to each test concentration. The study was repeated three times.

Immobilization was determined visually after 24 hours. Results showed that the EC50 for immobilization of the daphnids was 0.37 mg/L.

Data source

Reference

Reference Type:

publication

Title:

The Acute Toxicity of Surfactants on Fish Cells, Daphnia magna and Fish - A Comparative Study

Author:

Sandbacka, M., Christianson, I., Isomaa, B.

Year:

2000

Bibliographic source:

Toxicology in Vitro 14 (2000) pp. 61-68

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

Not specified

Deviations:

yes

Principles of method if other than guideline:

- Fish were exposed to the test substance for 24 hours instead of 96 hours (see 'Remarks on exposure duration').
- Test solutions were not anlaysed for actual exposure concentrations.

GLP compliance:

no

Remarks:

Publication in literature

Test material

Test material information

Constituent 1

Reference substance name:

Dodecyltrimethylammonium bromide

EC Number:

214-290-3

EC Name:

Dodecyltrimethylammonium bromide

Cas Number:

1119-94-4

Molecular formula:

C15H34N.Br

IUPAC Name:

dodecyltrimethylazanium bromide

Test material form:

not specified

Details on test material:

Type of substance: synthetic detergent
Supplier: Sigma Chemical Co. (St. Lous, MO, USA)
Molecular formula: C15H34NBr

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Details on test solutions:

No details provided.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source: obtained from a local fish farm
- Age at study initiation (mean and range, SD): not indicated
- Length at study initiation (length definition, mean, range and SD): 8.5 ± 0.4 cm
- Weight at study initiation (mean and range, SD): 6.6 ± 0.6 g

ACCLIMATION
- Acclimation period: 3 weeks
- Acclimation conditions:
* temperature: 12°C
* aeration: yes
* photoperiod (hrs light/hrs dark): 12/12
- Type and amount of food during acclimation: commercial rainbow trout pellets
- Feeding frequency during acclimation: daily

FEEDING DURING TEST: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Remarks on exposure duration:

An exposure period of 24 hours was determined based on the knowledge that toxicity of surfactants is usually expressed within the first 24 hours and because the Daphnia test in the same study was performed with the same exposure period.

Test conditions

Hardness:

49 mg CaCO3/L

Test temperature:

11.3 ± 0.9°C

pH:

7.8

Details on test conditions:

TEST SYSTEM
- Test vessel: 10-litre glass aquaria
- Aeration: yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: well water
- Culture medium different from test medium: no
- Intervals of water quality measurement: not indicated

OTHER TEST CONDITIONS: not indicated

EFFECT PARAMETERS MEASURED: mortality after 24 hours

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Key result

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

40.6 mg/L

Nominal / measured:

not specified

Basis for effect:

mortality (fish)

Details on results:

- Results were expressed in micromolar: LC50 = 131.6 ± 3.3 μM
- No abnormalities during the observations of the fish were reported.
- No details were provided concerning the basis of the effect levels. Since also no analytics were reported, it is assumed that the effect level was based on the nominal concentrations.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 for Dodecyltrimethylammonium bromide towards rainbow trout was 40.6 mg/L.

Executive summary:

The acute toxicity of Dodecyltrimethylammonium bromide towards rainbow trout was assessed in a study available in public literature, performed similar to OECD guideline 203. Deviating from the guideline was the shorter exposure period (24 hours) based on the knowledge that toxicity of surfactants is usually expressed within the first 24 hours. Also, test solutions were not analysed for actual exposure concentrations. A range of five different concentrations was tested and a control was included. Ten fish were exposed to each test concentration. The study was repeated three times.

Mortality was determined after 24 hours. Results showed that the LC50 for Dodecyltrimethylammonium bromide towards rainbow trout was 40.6 mg/L.