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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
8.9.2000 to 24.10.2000
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
not specified
Qualifier:
according to guideline
Guideline:
other: Test method of new chemical substance
Version / remarks:
Kanpogyo No. 5, Yakuhatsu No. 615 and 49 Kikyoku No. 392 dated 13th July 1974
Deviations:
not specified
GLP compliance:
yes
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Mitsuboshi Chemicals, Japan, KCL2287
- Expiration date of the lot/batch:
- Purity test date:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Refrigerated storage
- Stability under test conditions: IR spectrum before and after the experiment are collated and thus it was confirmed the stability of test substanceunder the storage condition.
- Solubility and stability of the test substance in the solvent/vehicle:
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: none
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:

FORM AS APPLIED IN THE TEST (if different from that of starting material)



Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Returned sludge from sewage treatment and Surface soil at water's edge contacting surface water and atmosphere.
- Laboratory culture:
- Method of cultivation: Approximately 30 minutes after stop the aeration into the cultivating vessel, approximately 1/3 of total supernatant liquid was removed. De-chlorinated water was added to the vessel to make total volume 10 L and re-aerated for more than 30 minutes and, artificial sewage of 50 g/L was added to the vessel to adjust concentration of artificial sewage to 0.1 wt% in the added de-chlorinated water. The same procedure was repeated once a day and activated sludge was provided after cultivating.
- Storage conditions:
- Storage length:
- Preparation of inoculum for exposure:
- Pretreatment:
- Concentration of sludge: 30 mg/l
- Initial cell/biomass concentration: no data
- Water filtered: yes
- Type and size of filter used, if any:
Duration of test (contact time):
>= 14 d
Initial conc.:
>= 100 mg/L
Based on:
not specified
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance:
aniline
Key result
Parameter:
% degradation (TOC removal)
Value:
>= 91
Sampling time:
14 d
Parameter:
other: BOD
Value:
>= 102
Sampling time:
14 d
Parameter:
other: HPLC
Value:
>= 100
Sampling time:
14 d
Results with reference substance:
It was confirmed that the test condition was valid, because the degree of degradation of aniline on Day7 and 14 determined by BOD was 75% and 76%, respectively

Analytical result of test liquid on Day 14

System

&

Vessel No.

Water + Test substance

Sludge + Test substance

Theoretical value

 1

 2

 3

 4

BOD*

mg

0

26

26.7

24.3

25.2

*

DOC residual amount and rate

mgC

8.9

0.7

0.9

0.7

9.5

%

94

7

10

8

-

Residual amount of test substance and residual rate

(HPLC)

mg

29.6

0

0

0

30.0

%

(1)

99

0

0

0

-

*Value of (Sludge + Test substance) system was shown after deduction of value of blank system.

Validity criteria fulfilled:
not specified
Interpretation of results:
readily biodegradable
Conclusions:
The study shows that the degradation by BOD is 102%, result by TOC is 91% and result by HPLC is 100%.

Description of key information

The study shows that the degradation by BOD is 102%, result by TOC is 91% and result by HPLC is 100%.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable
Type of water:
freshwater

Additional information