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The analogue substance was tested for aquatic invertebrates and toxicity to aquatic algae (NONS dossier, 1999).

In the acute toxicity test to aquatic algae EC50, 72h >100 mg/l and the acute toxicity to aquatic invertebrates showed a EC50, 48h > 100 mg/l.

Based on the read across considerations same results apply to Reactive Orange 12:2.