1,6-dichloro-1,6-dideoxy-β-D-fructofuranosyl 4-chloro-4-deoxy-α-D-galactose

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983-06-03 to 1984-05-03

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

version: adopted 12 May 1981

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 153006
- Purity test date: 97.7%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
-- sample 1: in the dark at ambient temperature
-- sample 2: wrapped in aluminium at 4°C

Analytical monitoring:

yes

Details on sampling:

- Concentrations: control, 560, 1000, 1800 and 2400 mg/L
- Sampling method: samples were collected from each vessel at the beginning and the end of the test
- Sample storage conditions before analysis: frozen

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The appropriate weight of the test substance was placed into a volumetric flask and borehole water added (1 L). The content of the flask was equally divided and added to two vessels already containing 5 L borehole water. The preparation flasks were rinsed with water and the rinsing divided equally between the test vessels. Finally the volume of solution in each test vessel was made up to 10 L.
- Controls: 10 L dilution water was added directly to the control vessels
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): minute black particles were observed in concentrations 1800 mg/L and 2400 mg/L

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source:
-- for preliminary and first definitive test: WRC Stevenage laboratory;
-- for second definitive test: Padworth Fisheries, Berkshire
- Weight at study initiation (mean of 10 fish): preliminary test: 0.85 g; first definitive test: 0.63 g; second definitive test: 0.86 g
- Maintenance of the brood fish: daily feeding with trout pellets (EWOS-BAKER Ltd., Size 2 or BP Nutrition Ltd, Mainstream Trout Fry 02) equivalent to 1% of the total weight of the fish in the holding tank

ACCLIMATION
- Acclimation period: 24 to 30 h prior starting of test
- Acclimation conditions (same as test or not): same as test
- Type and amount of food during acclimation: no feeding during acclimation
- Mortality during 14-day period prior to transferring a batch of fish to acclimation tank: preliminary test: 2.6%; first definitive test: 2.3%; second definitive test: 0.1%

Test type:

static

Water media type:

other: borehole water

Limit test:

no

Total exposure duration:

96 h

Hardness:

preliminary: 223 - 234 mg/L as CaCO3
first definitive: 200 - 240 mg/L as CaCO3
second definitive: 204 - 230 mg/L as CaCO3

Test temperature:

preliminary: 15.4 - 16.4°C
first definitive: 13.5 - 14.3°C
second definitive: 14.2 - 15.2°C

pH:

preliminary: 8.08 - 8.27
first definitive: 8.16 - 8.35
second definitive: 8.14 - 8.32

Dissolved oxygen:

preliminary: 91 - 98% ASV (air saturation value)
first definitive: 82 - 95% ASV
second definitive: 84 - 91% ASV

Nominal and measured concentrations:

preliminary test:
- nominal: control, 560, 1000 mg/L
first definitive test:
- nominal: control, 560, 1000, 1800 mg/L
second definitive test:
- nominal: control, 560, 1000, 1800, 2400 mg/L
- measured:

Details on test conditions:

TEST SYSTEM
- Test vessel: 14 L all-glass aquaria
- Material, size, headspace, fill volume: 10 L
- Aeration: through Pasteur pipette connected to an oil-free supply of compressed air
- No. of organisms per vessel: preliminary test: 5; definitive tests: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: borehole water
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Photoperiod: 14h light, 10h dark, during second definitive test: each 30 minutes dawn and dusk

TEST CONCENTRATIONS
- A second definitive test was conducted to include a higher concentration.

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: preliminary test

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1 800 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: first definitive test

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 2 400 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: second definitive test

Details on results:

- Behavioural abnormalities: some fish were overturned or swimming spirally in concentrations down to 560 mg/L until the end of the test (96 h)
- Other biological observations: some fish in all concentrations of the second definitive test showed darker pigmentation and loss of balance when swimming. The number of affected fish increased with concentration.
- Mortality of control: no mortality in any control group
- Other adverse effects control: no adverse effects were observed in the control groups.

Sublethal observations / clinical signs:

preliminary test:

nominal concentration [mg/L]	number exposed	mortality after 96h [%]
control	10	0
560	10	0
1000	10	10

first definitive test:

test vessel number	nominal concentration [mg/L]	number exposed	mortality observations [number of died fish]
			2h	16h	24h	48h	72h	96h
1	control	10	0	0	0	0	0	0
2	control	10	0	0	0	0	0	0
3	560	10	0	0	0	0	0	0
4	560	10	0	0	0	0	0	1
5	1000	10	0	0	0	0	1	3
6	1000	10	0	0	0	1	2	3
7	1800	10	0	0	0	1	4	7
8	1800	10	0	0	0	1	2	3

second definitive test:

test vessel number	nominal concentration [mg/L]	number exposed	mortality observations [number of died fish]
			15h	24h	48h	72h	96h
1	control	10	0	0	0	0	0
2	control	10	0	0	0	0	0
3	560	10	0	0	0	0	0
4	560	10	0	0	0	0	0
5	1000	10	0	0	0	0	0
6	1000	10	0	0	0	0	0
7	1800	10	0	0	0	0	0
8	1800	10	0	0	0	0	0
9	2400	10	0	0	0	0	2
10	2400	10	0	0	0	0	2

Validity criteria fulfilled:

yes

Conclusions:

The toxicity of the test item on fish (Rainbow trout) was determined in a GLP study according to OECD 203. The 96-h EC50 was determined to be 1800 mg/L (nominal).

Description of key information

The toxicity of the test item on fish (Rainbow trout) was determined in a GLP study according to OECD 203. The 96-h EC50 was determined to be 1800 mg/L (nominal).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 800 mg/L

Additional information

The toxicity of test item on the fish Oncorhynchus mykiss (Rainbow trout) was determined in a GLP study according to OECD 203 covering 2 definitive tests. Individuals of Rainbow trout exposed to 0 (control), 560, 1000, 1800 and 2400 mg/L of the test substance in chalk borehole water, equivalent to geometric mean measured concentrations of <LOQ, 543,998, 1778 and 2414 mg/L during the incubation of 96 h. Mortality of Rainbow trout was observed after 48 h at 1000 mg/L in the first definitive test and after 96 h at 2400 mg/L in the second definitive test. The 96 h EC50 value of >1800 mg/L (nominal) was concluded.