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EC number: 286-839-5
CAS number: 85391-83-9
preliminary range finding test was conducted at the test facility under
static conditions over a period of 48 hours with four nominal test item
concentrations of 0.1, 1, 10 and 100 mg/L prepared with dilution water.
The test item solutions were visually clear and concentration dependent
red to orange coloured.
preliminary range finding test was conducted under diffuse light
conditions (light intensity of max. 1500 lx, 16/8 hours light/dark
cycle). In the range finding test, two replicates per concentration
level and control, each with five daphnids, were tested.
Rates in the non-GLP Preliminary Range Finding Test
(n = 10,
divided into 2 replicates with 5 daphnids each)
Range Finding Test
Start of the exposure, 0 hours
End of the Exposure, 48 hours
Nominal test item
percent of the nominal concentration of the test item
Lowest Calibration Level (0.05 mg/L of the test item)
In the acute immobilization test with Daphnia
magna (STRAUS), the effects of the test item Reactive Red 123 were
determined at the test facility according to OECD 202 (2004) from
2018-06-05 to 2018-06-07.
The study was conducted under static
conditions over a period of 48 hours with five concentration levels
prepared by dilution from the stock solution of the test item (nominal
concentration 100 mg/L). The tested concentration levels were 6.25 -
12.5 - 25.0 - 50.0 - 100 mg/L.
itemis a red powder with
awater solubility of 50 g/L
(25 °C). The test item concentrations showed aconcentration-dependent
red to orange colouringand were visually clear throughout the exposure
period. The control was colourless and visually clear as well.
daphnids (divided into 4 replicates with 5 daphnids each) were exposed
to each concentration leveland the control.
The concentrationsof the test item were
analytically verified via HPLC-DAD in fresh media at the
start of the exposure (0 hours) and in old media at the end of the
exposure period (48 hours) in all tested concentration levels and in the
The measured concentrations of the
test item in fresh media at the start of the exposure (0 hours)were in
the range of 96 to 99% of the nominal values. At the end of the exposure
period (48 hours), the measured test item concentrations
in the old media were in
the range of 95 to 98% of the nominal values. Since the
measured concentrations of the test item Reactive Red 123 remained
stable within ± 20% of the nominal concentrations throughout the
exposure period, the nominal concentrations were used for evaluation.
All effect concentrations (EC10
/ 50 / 100) are based on the nominal concentrations
of the test item Reactive Red 123.
criteria of the test guideline were fulfilled.
on the nominal concentration of the test item)
Reactive Red 123
Nominal test item concentration
[mg test item/L]
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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