Pyridine-2-carbonitrile

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1979-06-12 to 1979-07-03

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

not specified

Principles of method if other than guideline:

no specific method information available

GLP compliance:

no

Remarks:

predates GLP

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Pyridine-2-carbonitrile

EC Number:

202-880-3

EC Name:

Pyridine-2-carbonitrile

Cas Number:

100-70-9

Molecular formula:

C6H4N2

IUPAC Name:

pyridine-2-carbonitrile

Test material form:

solid

Specific details on test material used for the study:

lot # 5257; order # 02166

Test animals

Species:

rat

Strain:

Wistar

Remarks:

SPF, HAN 75

Sex:

male/female

Details on test animals or test system and environmental conditions:

30 male, 30 female. Acclimatiziation period = 7 days, room temp 22-24°C, relative humidity = 42-65%, body weight at start of trial = 90 - 118 g; 1 animal/cage, food = pelletted Altromin R, period without food prior to administration = 17-18 hours

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Remarks:

pH 7.62 suspension in water

Details on oral exposure:

5 g in 100 mL

Doses:

0 (control), 0.5, 0.75, 1.0, 1.25, 1.5 g/kg

No. of animals per sex per dose:

10 animals/dose

Control animals:

yes

Details on study design:

observation period = 22 days

Statistics:

probit analysis

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

1 000 mg/kg bw

Based on:

test mat.

95% CL:

>= 900 - <= 1 100

Mortality:

Mortality at 1000 mg/kg (4/10 by day 22); at 1250 (9/10 by day 22); at 1500 (10/10 by day 4)

Clinical signs:

other: At 1000 mg/kg doses and higher: moderate to severe apathy, lateral position, unconsciousness, high-legged walk, increase lacrimal secretion, increased salivary secretion, abdominal position.

Gross pathology:

Animals dying before end of observation period: suspected slight local irritation of the gastrointestinal tract (multiple petechial bleeding of the glandular part of the gastic mucosa, watery content of the small intesting) as well as supsected slight alterations of the kidneys (pale coloration).
Animals sacrificed at end of observation period: no pathological findings observed

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

LD50 of 2 -Cyanopyridine in rats = 1000 mg/kg (oral gavage)

Executive summary:

LD50 of 2 -Cyanopyridine in rats = 1000 mg/kg (oral gavage). Meets Acute Oral Category 4 criteria.