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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1979-06-12 to 1979-07-03
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979
Report date:
1979

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
not specified
Principles of method if other than guideline:
no specific method information available
GLP compliance:
no
Remarks:
predates GLP
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Pyridine-2-carbonitrile
EC Number:
202-880-3
EC Name:
Pyridine-2-carbonitrile
Cas Number:
100-70-9
Molecular formula:
C6H4N2
IUPAC Name:
pyridine-2-carbonitrile
Test material form:
solid
Specific details on test material used for the study:
lot # 5257; order # 02166

Test animals

Species:
rat
Strain:
Wistar
Remarks:
SPF, HAN 75
Sex:
male/female
Details on test animals or test system and environmental conditions:
30 male, 30 female. Acclimatiziation period = 7 days, room temp 22-24°C, relative humidity = 42-65%, body weight at start of trial = 90 - 118 g; 1 animal/cage, food = pelletted Altromin R, period without food prior to administration = 17-18 hours

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
water
Remarks:
pH 7.62 suspension in water
Details on oral exposure:
5 g in 100 mL
Doses:
0 (control), 0.5, 0.75, 1.0, 1.25, 1.5 g/kg
No. of animals per sex per dose:
10 animals/dose
Control animals:
yes
Details on study design:
observation period = 22 days
Statistics:
probit analysis

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
1 000 mg/kg bw
Based on:
test mat.
95% CL:
>= 900 - <= 1 100
Mortality:
Mortality at 1000 mg/kg (4/10 by day 22); at 1250 (9/10 by day 22); at 1500 (10/10 by day 4)
Clinical signs:
At 1000 mg/kg doses and higher: moderate to severe apathy, lateral position, unconsciousness, high-legged walk, increase lacrimal secretion, increased salivary secretion, abdominal position.
Body weight:
Day 22 bodyweights: 244 g (male); 189 g (female) for control group; 250 g (male); 177 mg (female) for 1000 mg/kg group
Gross pathology:
Animals dying before end of observation period: suspected slight local irritation of the gastrointestinal tract (multiple petechial bleeding of the glandular part of the gastic mucosa, watery content of the small intesting) as well as supsected slight alterations of the kidneys (pale coloration).
Animals sacrificed at end of observation period: no pathological findings observed

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Conclusions:
LD50 of 2 -Cyanopyridine in rats = 1000 mg/kg (oral gavage)
Executive summary:

LD50 of 2 -Cyanopyridine in rats = 1000 mg/kg (oral gavage). Meets Acute Oral Category 4 criteria.