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Biodegradation in water

Estimation Programs Interface Suite (2018) was run to predict the biodegradation potential of the test compound 1,3-Benzenediamine, coupled with diazotized m-phenylenediamine, acetates (CAS no.  84281 -74 -3) in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical 1,3-Benzenediamine, coupled with diazotized m-phenylenediamine, acetates is expected to be not readily biodegradable.

Additional information

Biodegradation in water

Predicted data for the target compound 1,3-Benzenediamine, coupled with diazotized m-phenylenediamine, acetates (CAS No. 84281-74-3) and various supporting weight of evidence studies for its structurally and functionally similar read across substance were reviewed for the biodegradation end point which are summarized as below:

 

In a prediction using the Estimation Programs Interface Suite, the biodegradation potential of the test compound 1,3-Benzenediamine, coupled with diazotized m-phenylenediamine, acetates (CAS no.  84281 -74 -3) in the presence of mixed populations of environmental microorganisms was estimated. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical 1,3-Benzenediamine, coupled with diazotized m-phenylenediamine, acetates is expected to be not readily biodegradable.

 

In a supporting weight of evidence study from peer reviewed journal (Toshihide Saito et. al., 1984) for the test item,biodegradation experiment was conducted for evaluating the percentage biodegradability of test substance. Activated sludge was used as a test inoculum obtained from municipal sewage. Test substance of conc. 500 mg/l was prepared and diluted as needed.The COD measurement was performed by the potassium dichromate reflux method based on Japanese Industrial Standards. In the BOD measurement, a sample solution was taken into a container. Then JIS-BOD testing solutions, i.e. 3 ml of A solution and 1 ml of B, C, D solutions were added to the sample solution, respectively. Then 5 ml of the supernatant of the municipal sewage activated sludge was added to the sample solution and diluted to 300 ml with water. Immediately, the BOD-time curve was recorded at 20~ using an Ohkura OM-200I type coulometer. The TOC measurement was carried out by a Yanagimoto TOC-ILW.BOD5, COD, ThOD and TOC value of the test chemical was determined to be 0.021, 0.65, 1.23 and 0.20 g/g, respectively. The BOD5/TOC ratio of chemical was determined to be 0.11 (i.e. ranges in between 0.08-0.89), indicating that the chemical is highly resistant to aerobic biodegradation. Thus, based on this value BOD5/TOC value, it can be concluded that the chemical is not readily biodegradable in nature.

 

Another biodegradation study was carried out for 48 days for evaluating the percentage biodegradation of the test chemical using modified OECD Guideline 302B. Activated sludge was used as a test inoculum. The sources of the activated sludge were treatment plants conveniently located to the laboratories carrying out the test. These treatment plants received communal and/or industrial wastewater. Concentration of inoculum i.e, activated sludge used was 0.5 g/l and initial test substance conc. used in the study was 100 mg/l. Analytical methods involve the measurement of extinction at absorption maximum 412 nm and DOC (dissolved organic carbon).The percentage degradation of test substance was determined to be 5% by DOC removal. Based on the limit values for classification of Static Test – the test chemical was classified in CATEGORY ‘D’. Thus, the test substance is considered to be not readily biodegradable in nature.

 

For the test chemical,biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of test substance. The study was performed according to OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I) (authoritative databases, 2018). Activated sludge (non-adapted) was used as a test inoculums for the study. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance was determined to be 0% by BOD parameter in 14 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.

 

In a supporting weight of evidence study from authoritative databases (J-CHECK and EnviChem, 2018) for the test item,biodegradation experiment was conducted for 28 days for evaluating the percentage biodegradability of test substance. The study was performed according toOECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I). Activated sludge (non-adapted) was used as a test inoculums for the study. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance was determined to be 0% by BOD and HPLC parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in water.

 

On the basis of above results for target chemical 1,3-Benzenediamine, coupled with diazotized m-phenylenediamine, acetates(from modelling database, 2018), it can be concluded that the test substance 1,3-Benzenediamine, coupled with diazotized m-phenylenediamine, acetates can be expected to be not readily biodegradable in nature.