Registration Dossier
Registration Dossier
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EC number: 219-199-2 | CAS number: 2386-52-9
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / micronucleus study
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 017
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 487 (In vitro Mammalian Cell Micronucleus Test)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- in vitro mammalian cell micronucleus test
Test material
- Reference substance name:
- Silver nitrate
- EC Number:
- 231-853-9
- EC Name:
- Silver nitrate
- Cas Number:
- 7761-88-8
- Molecular formula:
- Ag.HNO3
- Test material form:
- solid
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- human lymphoblastoid cells (TK6)
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- not applicable
- Test concentrations with justification for top dose:
- 1.00-1.75 µg/ml
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- not specified
Results and discussion
Test results
- Key result
- Species / strain:
- human lymphoblastoid cells (TK6)
- Metabolic activation:
- not specified
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- Ag+ can cause genotoxicity via oxidative stress
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